Title:
LIGATION-BASED RNA AMPLIFICATION
Document Type and Number:
WIPO Patent Application WO2006071776
Kind Code:
A3
Abstract:
Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.
Inventors:
NELSON JOHN R (US)
DUTHIE R SCOTT (US)
DHULIPALA ROHINI (US)
GROSSMANN GREGORY A (US)
SEKHER ANURADHA (US)
DUTHIE R SCOTT (US)
DHULIPALA ROHINI (US)
GROSSMANN GREGORY A (US)
SEKHER ANURADHA (US)
Application Number:
PCT/US2005/046800
Publication Date:
September 14, 2006
Filing Date:
December 22, 2005
Export Citation:
Assignee:
GE HEALTHCARE BIO SCIENCES (US)
NELSON JOHN R (US)
DUTHIE R SCOTT (US)
DHULIPALA ROHINI (US)
GROSSMANN GREGORY A (US)
SEKHER ANURADHA (US)
NELSON JOHN R (US)
DUTHIE R SCOTT (US)
DHULIPALA ROHINI (US)
GROSSMANN GREGORY A (US)
SEKHER ANURADHA (US)
International Classes:
C12Q1/68; C12N15/10
Domestic Patent References:
| WO1999043850A1 | 1999-09-02 |
Other References:
ARNAUD-BARBE N ET AL: "Transcription of RNA templates by T7 RNA polymerase", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 15, 1 August 1998 (1998-08-01), pages 3550 - 3554, XP002305270, ISSN: 0305-1048
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