Title:
LIGHT EMISSION OBSERVATION METHOD
Document Type and Number:
WIPO Patent Application WO/2016/203617
Kind Code:
A1
Abstract:
This light emission observation method comprises the steps of: maintaining a biological sample in a viable state, the biological sample containing an observation cell expressing a gene for a first photoprotein; preparing a reference that displays a reference amount of the first photoprotein so that when the observation cell is photographed, the reference is included in one field of view with the observation cell; applying a predetermined stimulation to the observation cell and the reference to cause a luminescent reaction in the observation cell and the reference; and acquiring a plurality of wavelength band images by photographing light emitted from the observation cell and the reference in mutually different wavelength bands. The light emission observation method further comprises steps of: determining, from the plurality of the wavelength band images, the amount of light emitted from the observation cell and the amount of light emitted from the reference; calculating a correction coefficient using the determined amount of light emitted from the reference; and correcting the determined amount of light emitted from the observation cell using the correction coefficient.
Inventors:
OGO KATSUNORI (JP)
Application Number:
PCT/JP2015/067615
Publication Date:
December 22, 2016
Filing Date:
June 18, 2015
Export Citation:
Assignee:
OLYMPUS CORP (JP)
International Classes:
C12Q1/02; C12Q1/66; G01N21/64; G01N21/76; G01N21/78
Foreign References:
| JP2009156715A | 2009-07-16 | |||
| JP2007327928A | 2007-12-20 | |||
| JP2007232631A | 2007-09-13 | |||
| JPH1033159A | 1998-02-10 | |||
| JPH0538297A | 1993-02-19 | |||
| JPS61228349A | 1986-10-11 | |||
| JP2014161321A | 2014-09-08 |
Attorney, Agent or Firm:
KURATA, Masatoshi et al. (JP)
Masatoshi Kurata (JP)
Masatoshi Kurata (JP)
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