Title:
A LINCOSAMIDE UNIVERSAL MONOCLONAL ANTIBODY HYBRIDOMA CELL STRAIN AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/129082
Kind Code:
A1
Abstract:
Disclosed is a lincosamide universal monoclonal antibody hybridoma cell strain and application thereof, belonging to the technical field of food safety immunological detection. The invention adopts chlorine-substituted clindamycin derivative as a hapten, and the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immune antigen. The immune antigen is uniformly mixed with Freund's adjuvant and used to immunize a BALB/c mouse by subcutaneous injection. The clindamycin is coupled with ovalbumin (OVA) by a carbonyldiimidazole (CDI) method as a coating antigen for mouse serum and cell supernatant detection. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and subjected to indirect ELISA, indirect competitive ELISA screening and three subclones to obtain a population-selective hybridoma cell strain. The cell strain provided by the invention has excellent inhibitory effect on clindamycin, lincomycin and pirlimycin, and can meet the market demand for lincosamide multi-residue immunoassay products.
Inventors:
KUANG HUA (CN)
GUO LINGLING (CN)
XU CHUANLAI (CN)
XU LIGUANG (CN)
MA WEI (CN)
LIU LIQIANG (CN)
SONG SHANSHAN (CN)
WU XIAOLING (CN)
GUO LINGLING (CN)
XU CHUANLAI (CN)
XU LIGUANG (CN)
MA WEI (CN)
LIU LIQIANG (CN)
SONG SHANSHAN (CN)
WU XIAOLING (CN)
Application Number:
PCT/CN2018/123952
Publication Date:
July 04, 2019
Filing Date:
December 26, 2018
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N5/20; C07K16/44; G01N33/577
Domestic Patent References:
| WO2016044588A1 | 2016-03-24 |
Foreign References:
| CN108165532A | 2018-06-15 | |||
| CN104004718A | 2014-08-27 | |||
| CN104897896A | 2015-09-09 | |||
| CN101256188A | 2008-09-03 | |||
| CN101726590A | 2010-06-09 |
Other References:
HE, JIE ET AL.: "Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues", MEAT SCIENCE, vol. 125, 1 December 2016 (2016-12-01), XP029859473, ISSN: 0309-1740, DOI: 10.1016/j.meatsci.2016.11.024
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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