Title:
LIPASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/035357
Kind Code:
A1
Abstract:
Provided are a lipase mutant and an application thereof. Specifically, one or more mutations selected from A262H, A338V, V364I, A158P/V, and I159N are generated on the basis of an amino acid sequence as shown in SEQ ID NO: 1; compared with parental lipase, a change in the structure and function of a protein occurs in the lipase mutant, and the stereoselectivity is improved, such that the dosage of an enzyme is decreased to a certain extent, the post-treatment difficulty is reduced, and the lipase mutant is suitable for industrial production.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
LIU FANG (CN)
JIA RU (CN)
LIU WENJING (CN)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
LIU FANG (CN)
JIA RU (CN)
LIU WENJING (CN)
Application Number:
PCT/CN2021/123607
Publication Date:
March 16, 2023
Filing Date:
October 13, 2021
Export Citation:
Assignee:
ASYMCHEM LIFE SCIENCE TIANJIN CO LTD (CN)
International Classes:
C12N9/20; C12N1/21; C12N15/55; C12N15/70; C12P7/02; C12P7/40; C12R1/19
Foreign References:
| CN112375751A | 2021-02-19 | |||
| CN106459939A | 2017-02-22 | |||
| CN111218437A | 2020-06-02 | |||
| CN108642025A | 2018-10-12 |
Other References:
DATABASE Protein NCBI; ANONYMOUS : "esterase [Pseudomonas putida]", XP093044949
CHEN SHAO-XIN, SHI BING-ZHAO: "Screening of strain producing an novel esterase with high enantioselectivity and molecular cloning of the enzyme gene", ACTA MIEROBIOLOGICA SINICA, KEXUE CHUBANSHE, BEIJING, CN, vol. 47, no. 3, 4 June 2007 (2007-06-04), CN , pages 452 - 455, XP093045023, ISSN: 0001-6209, DOI: 10.13343/j.cnki.wsxb.2007.03.015
DATABASE Protein NCBI; ANONYMOUS : "beta-lactamase family protein [Pseudomonas putida]", XP093045026
"Application of Biocatalyst in Chiral Drug Synthesis [J", AMINO ACIDS AND BIOLOGICAL RESOURCES, vol. 35, no. 4, 2013, pages 39 - 42
"Research Progress on Immobilization and Chiral Resolution Thereof of Lipase [J", APPLIED CHEMISTRY, vol. 40, no. 10, 2011, pages 1823 - 1827
CHEN SHAO-XIN, SHI BING-ZHAO: "Screening of strain producing an novel esterase with high enantioselectivity and molecular cloning of the enzyme gene", ACTA MIEROBIOLOGICA SINICA, KEXUE CHUBANSHE, BEIJING, CN, vol. 47, no. 3, 4 June 2007 (2007-06-04), CN , pages 452 - 455, XP093045023, ISSN: 0001-6209, DOI: 10.13343/j.cnki.wsxb.2007.03.015
DATABASE Protein NCBI; ANONYMOUS : "beta-lactamase family protein [Pseudomonas putida]", XP093045026
"Application of Biocatalyst in Chiral Drug Synthesis [J", AMINO ACIDS AND BIOLOGICAL RESOURCES, vol. 35, no. 4, 2013, pages 39 - 42
"Research Progress on Immobilization and Chiral Resolution Thereof of Lipase [J", APPLIED CHEMISTRY, vol. 40, no. 10, 2011, pages 1823 - 1827
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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