Title:
LIPASE PRODUCTION AND PURIFICATION
Document Type and Number:
WIPO Patent Application WO/2011/081513
Kind Code:
A3
Abstract:
The invention provides a process for extractive fermentation using integrated aqueous two-phase system for the simultaneous upstream of microbial fermentation and downstream processing of extracellular lipase derived from Burkholderia sp. The medium used for this extractive fermentation comprises components of fermentation media and a composition for aqueous two-phase system (ATPS). The two-phase fermentation system was used to extract extracellular lipase, which is produced continuously from the bacteria. The extractive fermentation includes the steps of (a) preparing an immiscible medium, wherein the medium includes fermentation media and ATPS forming reagents; (b) inoculating Burkholderia sp. into the medium as prepared in step (a); (c) agitating the medium prepared from step (b); (d) separating the medium from step (c) into two-phases for extracellular lipase purification; and (e) recovering the extracellular lipase from the medium.
Inventors:
LING TAU CHUAN (MY)
OOI CHIEN WEI (MY)
TEY BENG TI (MY)
ARIFF ARBAKARIYA (MY)
HII SIEW LING (MY)
OOI CHIEN WEI (MY)
TEY BENG TI (MY)
ARIFF ARBAKARIYA (MY)
HII SIEW LING (MY)
Application Number:
PCT/MY2010/000256
Publication Date:
September 09, 2011
Filing Date:
November 09, 2010
Export Citation:
Assignee:
UNIV PUTRA MALAYSIA (MY)
LING TAU CHUAN (MY)
OOI CHIEN WEI (MY)
TEY BENG TI (MY)
ARIFF ARBAKARIYA (MY)
HII SIEW LING (MY)
LING TAU CHUAN (MY)
OOI CHIEN WEI (MY)
TEY BENG TI (MY)
ARIFF ARBAKARIYA (MY)
HII SIEW LING (MY)
International Classes:
C12N9/20; C12N1/20; C12P21/00
Foreign References:
| US5882520A | 1999-03-16 | |||
| US20090023902A1 | 2009-01-22 |
Other References:
CHEN, J.-P. ET AL.: "Enhanced production of Serratia marcescens chitinase in PEG/dextran aqueous two-phase systems", ENZYME AND MICROBIAL TECHNOLOGY, vol. 17, no. 11, November 1995 (1995-11-01), pages 1021 - 1027
OOI, C. W. ET AL.: "Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems", PROCESS BIOCHEMISTRY, vol. 44, 27 May 2009 (2009-05-27), pages 1083 - 1087
OOI, C. W. ET AL.: "Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems", PROCESS BIOCHEMISTRY, vol. 44, 27 May 2009 (2009-05-27), pages 1083 - 1087
Attorney, Agent or Firm:
DAMODHARAN, Ramakrishna (Suite 8-7-2 Menara Mutiara Bangsar,Ja Ian Liku Off JaIan Rion, Bangsar Kuala Lumpur, MY)
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