Title:
LIVER PRECURSOR-LIKE CELL MODEL DERIVED FROM PRIMARY HEPATOCYTE FOR HEPATITIS B VIRUS INFECTION, PREPARATION METHOD AND USE
Document Type and Number:
WIPO Patent Application WO/2020/056935
Kind Code:
A1
Abstract:
Provided are a liver precursor-like cell model derived from a primary hepatocyte for hepatitis B virus infection, a preparation method and the use, belonging to the field of hepatitis B virus-infected cell models in life sciences and medicine. The liver precursor-like cell model consists of functional liver cells which have been subjected to three-dimensional differentiation, wherein the functional liver cells which have been subjected to three-dimensional differentiation are obtained by the three-dimensional cultivation and liver maturation cultivation of liver precursor-like cells obtained after in-vitro transformation and cultivation of human primary hepatocytes. Through experimental verification, after the liver precursor-like cell model is infected with HBV, HBV infection-related genes such as RXRA, HNF4A and NTCP can be highly expressed, and the cell model can be used in hepatitis B virus infection research or can be co-cultured with HBV for preparing a hepatitis B virus infection cell model.
Inventors:
YAN HEXIN (CN)
ZENG MIN (CN)
FU GONGBO (CN)
ZHOU XU (CN)
HUANG WEIJIAN (CN)
WU HONGPING (CN)
WANG HONGYANG (CN)
ZENG MIN (CN)
FU GONGBO (CN)
ZHOU XU (CN)
HUANG WEIJIAN (CN)
WU HONGPING (CN)
WANG HONGYANG (CN)
Application Number:
PCT/CN2018/120207
Publication Date:
March 26, 2020
Filing Date:
December 11, 2018
Export Citation:
Assignee:
SECOND MILITARY MEDICAL UNIV (CN)
International Classes:
A61K31/7105; C12N5/071; A61P31/20; C12Q1/02; C12Q1/70
Domestic Patent References:
| WO2012005760A1 | 2012-01-12 |
Foreign References:
| CN105561342A | 2016-05-11 | |||
| CN106754636A | 2017-05-31 | |||
| CN108431209A | 2018-08-21 | |||
| CN103237888A | 2013-08-07 | |||
| CN103097517A | 2013-05-08 | |||
| CN105154386A | 2015-12-16 | |||
| US20070148767A1 | 2007-06-28 | |||
| CN105121632A | 2015-12-02 |
Other References:
FU, G. B. ET AL.: "Expansion and differentiation of human hepatocyte-derived liver progenitor- like cells and their use for the study of hepatotropic pathogens", CELL RESEARCH, vol. 29, no. 1, 25 October 2018 (2018-10-25), pages 8 - 22, XP036667414, ISSN: 1001-0602, DOI: 10.1038/s41422-018-0103-x
WU, H. ET AL.: "Reversible transition between hepatocytes and liver progenitors for in vitro hepatocyte expansion", CELL RESEARCH, vol. 27, 4 April 2017 (2017-04-04), pages 709 - 712, XP055694181, ISSN: 1001-0602, DOI: 10.1038/cr.2017.47
WU, H. ET AL.: "Reversible transition between hepatocytes and liver progenitors for in vitro hepatocyte expansion", CELL RESEARCH, vol. 27, 4 April 2017 (2017-04-04), pages 709 - 712, XP055694181, ISSN: 1001-0602, DOI: 10.1038/cr.2017.47
Attorney, Agent or Firm:
SHANGHAI SUNHOLD LAW FIRM (CN)
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