Title:
LONG-ACTING INTERLEUKIN-15 FUSION PROTEIN, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/141771
Kind Code:
A1
Abstract:
Provided is a long-acting IL-15 fusion protein, comprising a monomer fragment Fc, an IL-15 receptor α "Sushi" domain, and an IL-15 functional fragment. The IL-15 fusion protein has a higher yield than a Fc fusion protein, and can reduce the number of Treg cells in a tumor tissue, effectively activate an immune system to kill tumor cells, and improve the in vivo tumor suppression effect of IL-15.
Inventors:
JI EN (CN)
WANG CHUNYU (CN)
WU YANLING (CN)
WANG CHUNYU (CN)
WU YANLING (CN)
Application Number:
PCT/CN2021/076670
Publication Date:
July 07, 2022
Filing Date:
February 18, 2021
Export Citation:
Assignee:
SUZHOU FORLONG BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07K19/00; A61K38/20; A61K47/64; A61P35/00; C12N15/62
Domestic Patent References:
| WO2016095642A1 | 2016-06-23 |
Foreign References:
| CN106380521A | 2017-02-08 | |||
| CN107880136A | 2018-04-06 | |||
| CN111132733A | 2020-05-08 | |||
| CN112105645A | 2020-12-18 | |||
| CN109705211A | 2019-05-03 | |||
| CN202011161007A | 2020-10-27 |
Other References:
KAI-PING HAN; XIAOYUN ZHU; BAI LIU; EMILY JENG; LIN KONG; JASON L. YOVANDICH; VINAY V. VYAS; WARREN D. MARCUS; PIERRE-ANDRE CHAVAI: "IL-15:IL-15 receptor alpha superagonist complex: High-level co-expression in recombinant mammalian cells, purification and characterization", CYTOKINE, vol. 56, no. 3, 28 September 2011 (2011-09-28), US , pages 804 - 810, XP028115119, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2011.09.028
W. XU, M. JONES, B. LIU, X. ZHU, C. B. JOHNSON, A. C. EDWARDS, L. KONG, E. K. JENG, K. HAN, W. D. MARCUS, M. P. RUBINSTEIN, P. R. : "Efficacy and Mechanism-of-Action of a Novel Superagonist Interleukin-15: Interleukin-15 Receptor Su/Fc Fusion Complex in Syngeneic Murine Models of Multiple Myeloma", CANCER RESEARCH, vol. 73, no. 10, 15 May 2013 (2013-05-15), pages 3075 - 3086, XP055151674, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-12-2357
J G GIRI ET AL., EMBO J., vol. 13, no. 12, 15 June 1994 (1994-06-15), pages 2822 - 2830
EW MARTIN: "Remington's Pharmaceutical Sciences", 1975, MACK PUBLISHING CO.
YING, TIANLEI ET AL., JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, no. 23, 2012, pages 19399 - 19408
SANTOS-SAVIO A. ET AL., BIOTECN APLIC., vol. 17, 2000, pages 221 - 4
MOSSMAN T., J. IMMUNOL. METHODS., vol. 65, no. 1-2, 1983, pages 55 - 63
W. XU, M. JONES, B. LIU, X. ZHU, C. B. JOHNSON, A. C. EDWARDS, L. KONG, E. K. JENG, K. HAN, W. D. MARCUS, M. P. RUBINSTEIN, P. R. : "Efficacy and Mechanism-of-Action of a Novel Superagonist Interleukin-15: Interleukin-15 Receptor Su/Fc Fusion Complex in Syngeneic Murine Models of Multiple Myeloma", CANCER RESEARCH, vol. 73, no. 10, 15 May 2013 (2013-05-15), pages 3075 - 3086, XP055151674, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-12-2357
J G GIRI ET AL., EMBO J., vol. 13, no. 12, 15 June 1994 (1994-06-15), pages 2822 - 2830
EW MARTIN: "Remington's Pharmaceutical Sciences", 1975, MACK PUBLISHING CO.
YING, TIANLEI ET AL., JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, no. 23, 2012, pages 19399 - 19408
SANTOS-SAVIO A. ET AL., BIOTECN APLIC., vol. 17, 2000, pages 221 - 4
MOSSMAN T., J. IMMUNOL. METHODS., vol. 65, no. 1-2, 1983, pages 55 - 63
Attorney, Agent or Firm:
BEIJING TRUST OF ZHIGUO IP AGENCY CO., LTD. (CN)
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