Title:
LOW COST MANUFACTURE OF OLIGOSACCHARIDES
Document Type and Number:
WIPO Patent Application WO2000029603
Kind Code:
A3
Abstract:
This invention provides recombinant cells, reaction mixtures, and methods that are useful for the enzymatic synthesis of product saccharides. The recombinant cells contain a heterologous gene that encodes a glycosyltransferase which catalyzes at least one step of the enzymatic synthesis, as well a system for generating a nucleotide sugar that can serve as a substrate for the glycosyltransferase. The recombinant cells, reaction mixtures, and methods are useful for efficiently synthesizing a large variety of saccharides, including polysaccharides, oligosaccharides, glycoproteins and glycolipids, using relatively low-cost starting materials.
Inventors:
DEFREES SHAWN (US)
JOHNSON KARL (US)
JOHNSON KARL (US)
Application Number:
PCT/US1999/027599
Publication Date:
November 16, 2000
Filing Date:
November 18, 1999
Export Citation:
Assignee:
NEOSE TECHNOLOGIES INC (US)
DEFREES SHAWN (US)
JOHNSON KARL (US)
DEFREES SHAWN (US)
JOHNSON KARL (US)
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N9/10; C12P19/18; C12P19/28; (IPC1-7): C12N9/10; C12N1/20; C12N15/00; C07H21/04
Foreign References:
| US5705367A | 1998-01-06 | |||
| US5798233A | 1998-08-25 | |||
| US5541083A | 1996-07-30 | |||
| US5922577A | 1999-07-13 |
Other References:
KOIZUMI ET AL.: "Large scale production of UDP-galactose and globotriose by coupling metabolically engineered bacteria", NATURE BIOTECHNOLOGY,, vol. 16, no. 9, September 1998 (1998-09-01), pages 847 - 850, XP002929177
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