Title:
LOW-TEMPERATURE PRESERVATION SOLUTION FOR CELLS, SODIUM ALGINATE GEL, CELL GEL, PREPARATION METHOD, USE METHOD AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/062282
Kind Code:
A1
Abstract:
The present invention relates to the technical field of preservation, regeneration and repair of cells, and disclosed thereby is a low-temperature preservation solution for cells, mainly prepared from the following components in percent by mass: 0.8%-1.5% of mannitol, 2%-3% of human serum albumin, 3%-7% of low-molecular dextran, 0.1-0.3% of glutathione, 0.5%-2% of epigallocatechin gallate, 3%-5% of adenosine, 0.5%-1% of glucose, 0.8% of NaCl, 0.2% of KCl, 0.1% of CaCl2 and the balance of water. Also provided by the present invention is a sodium alginate gel, comprising sodium alginate, calcium chloride, hyaluronic acid and the low-temperature preservation solution for cells, and also provided thereby are a cell gel and a preparation method therefor as well as the application of the low-temperature preservation solution for cells, the sodium alginate gel and the cell gel. The present invention has the advantages of the low-temperature preservation solution for cells being able to maintain cell activity at 2-8°C for a long duration, the sodium alginate gel being able to be used as a temporary short-term preservation material for cells from various sources and brackets for cell tissue engineering, and the cell gel may be used as a medical wet wound dressing and may be used for repairing soft tissues, refractory-healing wound skins and mucosal wound injuries.
Inventors:
YANG YUAN (CN)
YUAN XIANG (CN)
HAN FEI (CN)
ZHANG ZHONGWEI (CN)
PAN HONGWEI (CN)
YUAN XIANG (CN)
HAN FEI (CN)
ZHANG ZHONGWEI (CN)
PAN HONGWEI (CN)
Application Number:
PCT/CN2018/109187
Publication Date:
April 02, 2020
Filing Date:
September 30, 2018
Export Citation:
Assignee:
CHENGDU YUANSHAN BIOTECHNOLOGY CO LTD (CN)
International Classes:
A01N1/02; A61K9/06; A61K35/28; A61L15/22
Foreign References:
| CN107926933A | 2018-04-20 | |||
| CN106538512A | 2017-03-29 | |||
| CN106110367A | 2016-11-16 | |||
| US20050233442A1 | 2005-10-20 |
Other References:
CHEN, LUPING ET AL.: "Comparison of the Effects of Different Cryoprotectants on Cryopreservation and Resuscitation of Human Induced Pluripotent Stem Cells", CHINESE JOURNAL OF CLINICAL NEUROSCIENCES, vol. 20, no. 5, 31 December 2012 (2012-12-31), pages 494 - 499
Attorney, Agent or Firm:
CHENGDU DINGFENG PATENT OFFICE (GENERAL PARTNER) (CN)
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