Title:
MEMS CHIP FOR CHROMATIN IMMUNOPRECIPITATION (ChIP) AND CHIP METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2010/059006
Kind Code:
A2
Abstract:
The present invention relates to an MEMS chip for chromatin immunoprecipitation (ChIP) which replaces conventional EP tubes used for ChIP experiments and which performs ChIP experiments, and to a ChIP method using same. The MEMS chip for ChIP comprises: an injection port through which a sample including a protein that is bound with DNA and antibody is introduced; a chamber communicated with the injection port, and having a plurality of beads positioned on the inner surface thereof, wherein a specific protein that binds with the antibody is fixed to the beads; and a discharge port communicated with the chamber, and formed on the other side of the injection port so as to discharge the introduced sample. Accordingly, the MEMS chip of ChIP can replace the conventional ChIP technology that uses EP tubes, and thus can carry out experiments with a small quantity of sample, with less amount of experimentation time, and have less fluctuation in experimental results, and also can carry out ChIP experiments even at room temperature.
Inventors:
OH HYUN JIK (KR)
YOON TAE JOONG (KR)
LEE SANG HOON (KR)
LEE BO YUN (KR)
HWANG EUL MOON (KR)
YOON TAE JOONG (KR)
LEE SANG HOON (KR)
LEE BO YUN (KR)
HWANG EUL MOON (KR)
Application Number:
PCT/KR2009/006903
Publication Date:
May 27, 2010
Filing Date:
November 23, 2009
Export Citation:
Assignee:
SEOULIN BIOSCIENCE CO LTD (KR)
OH HYUN JIK (KR)
YOON TAE JOONG (KR)
LEE SANG HOON (KR)
LEE BO YUN (KR)
HWANG EUL MOON (KR)
OH HYUN JIK (KR)
YOON TAE JOONG (KR)
LEE SANG HOON (KR)
LEE BO YUN (KR)
HWANG EUL MOON (KR)
International Classes:
G01N33/549; C12Q1/68; G01N33/68
Foreign References:
| KR100901995B1 | 2009-06-11 |
Other References:
'Miniaturized Systems for Chemistry and Life Sciences, 12th International Conference', October 2008 article OH, H. J. ET AL.: 'Microfluidic Device for DNA Enrichment and the Application of Chromatin Immunoprecipitation', pages 1075 - 1077
BUCK, M. J. ET AL.: 'ChIP-Chip: Consideration for the Design, Analysis, and Application of Genome-Wide Chromatin Immunoprecipitation Experiments' GENOMICS vol. 83, no. 3, March 2004, pages 349 - 360
PARK, P. J. ET AL.: 'Epigenetics Meets Nxxt-Generation Sequencing' EPIGENETICS vol. 3, no. 6, 22 November 2008, pages 318 - 321
PONZIELLI, R. ET AL.: 'Optimization of Experimental Design Parameters for High- Throughput Chromatin Immunoprecipitation Studies' NUCLEIC ACID RES. vol. 36, no. 21, 21 October 2008, page E144
BUCK, M. J. ET AL.: 'ChIP-Chip: Consideration for the Design, Analysis, and Application of Genome-Wide Chromatin Immunoprecipitation Experiments' GENOMICS vol. 83, no. 3, March 2004, pages 349 - 360
PARK, P. J. ET AL.: 'Epigenetics Meets Nxxt-Generation Sequencing' EPIGENETICS vol. 3, no. 6, 22 November 2008, pages 318 - 321
PONZIELLI, R. ET AL.: 'Optimization of Experimental Design Parameters for High- Throughput Chromatin Immunoprecipitation Studies' NUCLEIC ACID RES. vol. 36, no. 21, 21 October 2008, page E144
Attorney, Agent or Firm:
LEE, JAE IN (KR)
이재인 (KR)
이재인 (KR)
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