Title:
METHOD FOR AMPLIFYING iPS CELL SUITABLE FOR NEURAL DIFFERENTIATION, AND METHOD FOR INDUCING NEURAL STEM CELL
Document Type and Number:
WIPO Patent Application WO/2013/187416
Kind Code:
A1
Abstract:
The present invention relates to a method for increasing the neural differentiation ability of a pluripotent stem cell, which comprises culturing the pluripotent stem cell in the presence of a TGF-β family inhibitor, a GSK3β inhibitor and a BMP inhibitor. The present invention also relates to a method for inducing the differentiation of a pluripotent stem cell into a undifferentiated neural cell, which comprises culturing the pluripotent stem cell in the presence of a TGF-β family inhibitor and a GSK3β inhibitor under an oxygen partial pressure of 2 to 10%.
Inventors:
OKANO HIDEYUKI (JP)
AKAMATSU WADO (JP)
MATSUMOTO TAKUYA (JP)
SHOJI MASANOBU (JP)
NAKAMURA KOJI (JP)
AKAMATSU WADO (JP)
MATSUMOTO TAKUYA (JP)
SHOJI MASANOBU (JP)
NAKAMURA KOJI (JP)
Application Number:
PCT/JP2013/066102
Publication Date:
December 19, 2013
Filing Date:
June 11, 2013
Export Citation:
Assignee:
UNIV KEIO (JP)
TAKEDA PHARMACEUTICAL (JP)
TAKEDA PHARMACEUTICAL (JP)
International Classes:
C12N5/07; C12N5/0775; C12N5/0793; C12N5/0797
Domestic Patent References:
| WO2011019092A1 | 2011-02-17 | |||
| WO2011068879A2 | 2011-06-09 |
Foreign References:
| JP2012510805A | 2012-05-17 | |||
| JP2009539369A | 2009-11-19 | |||
| JP2002530068A | 2002-09-17 | |||
| JP2012507264A | 2012-03-29 |
Other References:
KRIKS, S. ET AL.: "Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease", NATURE, vol. 480, 2011, pages 547 - 551
SEKI, T. ET AL.: "Generation of Induced Pluripotent Stem Cells from Human Terminally Differentiated Circulating T Cells", CELL STEM CELL, vol. 7, 2010, pages 11 - 14
HANNA, J. ET AL.: "Direct Reprogramming of Terminally Differentiated Mature B Lymphocytes to Pluripotency", CELL, vol. 133, 2008, pages 250 - 264
RODRIGUES, C.A.V. ET AL.: "Hypoxia Enhances Proliferation of Mouse Embryonic Stem Cell-Derived Neural Stem Cells", BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 2, 2010, pages 260 - 270
MONDRAGON-TERAN, P. ET AL.: "Lowering Oxygen Tension Enhances the Differentiation of Mouse Embryonic Stem Cells into Neuronal Cells", BIOTECHNOL. PROG., vol. 25, 2009, pages 1480 - 1488
STACPOOLE, S.R.L. ET AL.: "Efficient derivation of NPCs, spinal motor neurons and midbrain dopaminergic neurons from hESCs at 3% oxygen", NATURE PROTOCOLS, vol. 6, no. 8, 2011, pages 1229 - 1240
TETSUHIRO KIKUCHI ET AL.: "Fuyu Baiyoho ni yoru Hito ES Oyobi iPS Saibo kara no Shinkei Bunka Yudo", REGENERATIVE MEDICINE, vol. 9, 2010, pages 163
INGRAHAM, C.A. ET AL.: "Matrix Metalloproteinase (MMP)-9 Induced by Wnt Signaling Increases the Proliferation and Migration of Embryonic Neural Stem Cells at Low 02 Levels", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 20, 2011, pages 17649 - 17657
KUNISADA, Y. ET AL.: "Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells", STEM CELL RESEARCH, vol. 8, March 2012 (2012-03-01), pages 274 - 284
SEKI, T. ET AL.: "Generation of Induced Pluripotent Stem Cells from Human Terminally Differentiated Circulating T Cells", CELL STEM CELL, vol. 7, 2010, pages 11 - 14
HANNA, J. ET AL.: "Direct Reprogramming of Terminally Differentiated Mature B Lymphocytes to Pluripotency", CELL, vol. 133, 2008, pages 250 - 264
RODRIGUES, C.A.V. ET AL.: "Hypoxia Enhances Proliferation of Mouse Embryonic Stem Cell-Derived Neural Stem Cells", BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 2, 2010, pages 260 - 270
MONDRAGON-TERAN, P. ET AL.: "Lowering Oxygen Tension Enhances the Differentiation of Mouse Embryonic Stem Cells into Neuronal Cells", BIOTECHNOL. PROG., vol. 25, 2009, pages 1480 - 1488
STACPOOLE, S.R.L. ET AL.: "Efficient derivation of NPCs, spinal motor neurons and midbrain dopaminergic neurons from hESCs at 3% oxygen", NATURE PROTOCOLS, vol. 6, no. 8, 2011, pages 1229 - 1240
TETSUHIRO KIKUCHI ET AL.: "Fuyu Baiyoho ni yoru Hito ES Oyobi iPS Saibo kara no Shinkei Bunka Yudo", REGENERATIVE MEDICINE, vol. 9, 2010, pages 163
INGRAHAM, C.A. ET AL.: "Matrix Metalloproteinase (MMP)-9 Induced by Wnt Signaling Increases the Proliferation and Migration of Embryonic Neural Stem Cells at Low 02 Levels", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 20, 2011, pages 17649 - 17657
KUNISADA, Y. ET AL.: "Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells", STEM CELL RESEARCH, vol. 8, March 2012 (2012-03-01), pages 274 - 284
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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