Title:
METHOD FOR MODULATING ACTIVITY OF Cas PROTEIN
Document Type and Number:
WIPO Patent Application WO/2020/059708
Kind Code:
A1
Abstract:
It was discovered that the endonuclease activity of a Cas protein can be modulated in a cell-cycle-dependent manner by causing a fusion protein of AcrIIA4 and Cdt1 to be expressed intracellularly together with the Cas protein.
Inventors:
NOMURA WATARU (JP)
MATSUMOTO DAISUKE (JP)
TAMAMURA HIROKAZU (JP)
MATSUMOTO DAISUKE (JP)
TAMAMURA HIROKAZU (JP)
Application Number:
PCT/JP2019/036372
Publication Date:
March 26, 2020
Filing Date:
September 17, 2019
Export Citation:
Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
UNIV HIROSHIMA (JP)
UNIV HIROSHIMA (JP)
International Classes:
C12N15/09; C12N15/62
Domestic Patent References:
| WO2017160689A1 | 2017-09-21 | |||
| WO2018009534A1 | 2018-01-11 | |||
| WO2008114544A1 | 2008-09-25 | |||
| WO2018197520A1 | 2018-11-01 | |||
| WO2019034784A1 | 2019-02-21 |
Other References:
RAUCH, B. J. ET AL.: "Inhibiton of CRISPR-Cas9 with bacteriophage proteins", CELL, vol. 168, 2017, pages 150 - 158, XP029882136
SHIN, J. ET AL.: "Disabling Cas9 by an anti- CRISPR DNA mimic", SCI. ADV., vol. 3, 2017, pages e1701620, XP055444909, DOI: 10.1126/sciadv.1701620
HOWDEN, S. E.: "A Cas9 variant for efficient generation of indel-free knockin or gene -corrected human pluripotent stem cells", STEM CELL REPORTS, vol. 7, 2016, pages 508 - 517, XP055379619, DOI: 10.1016/j.stemcr.2016.07.001
SHIN, J. ET AL.: "Disabling Cas9 by an anti- CRISPR DNA mimic", SCI. ADV., vol. 3, 2017, pages e1701620, XP055444909, DOI: 10.1126/sciadv.1701620
HOWDEN, S. E.: "A Cas9 variant for efficient generation of indel-free knockin or gene -corrected human pluripotent stem cells", STEM CELL REPORTS, vol. 7, 2016, pages 508 - 517, XP055379619, DOI: 10.1016/j.stemcr.2016.07.001
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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