Title:
METHOD FOR PRODUCING Fc-CONTAINING MOLECULE
Document Type and Number:
WIPO Patent Application WO/2023/167238
Kind Code:
A1
Abstract:
[Problem] To establish a method for producing an Fc-containing molecule having a highly pure and uniform sugar chain structure while using as small amount of sugar chain donor molecules as possible, etc., said method allowing free selection of the enzymes to be used therein from a wide range of options regardless of residual hydrolysis activity or enzyme species. [Solution] A novel method for producing an Fc-containing molecule having a desired N297-binding sugar chain, characterized by combining steps 1 and 2 that are described in detail in the present description, etc.
Inventors:
MUTO HIROSHI (JP)
IWAMOTO MITSUHIRO (JP)
ARAKAWA SHIORI (JP)
SAKAI YUTA (JP)
NARA KAORI (JP)
TANAKA YOSHIFUMI (JP)
NAKATANI YUKI (JP)
IWAMOTO MITSUHIRO (JP)
ARAKAWA SHIORI (JP)
SAKAI YUTA (JP)
NARA KAORI (JP)
TANAKA YOSHIFUMI (JP)
NAKATANI YUKI (JP)
Application Number:
PCT/JP2023/007583
Publication Date:
September 07, 2023
Filing Date:
March 01, 2023
Export Citation:
Assignee:
DAIICHI SANKYO CO LTD (JP)
International Classes:
C12P21/08; C07K1/18; C07K16/28; C12N9/24
Domestic Patent References:
| WO2018003983A1 | 2018-01-04 |
Foreign References:
| US20190040374A1 | 2019-02-07 | |||
| JP2017031132A | 2017-02-09 | |||
| JP2016082962A | 2016-05-19 |
Other References:
IWAMOTO MITSUHIRO, SEKIGUCHI YUKIKO, NAKAMURA KENSUKE, KAWAGUCHI YOSHIROU, HONDA TAKESHI, HASEGAWA JUN: "Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification", PLOS ONE, vol. 13, no. 2, 23 February 2018 (2018-02-23), pages e0193534, XP055907059, DOI: 10.1371/journal.pone.0193534
WANG SHIYI, IONESCU ROXANA, PEEKHAUS NORBERT, LEUNG JIN-YU, HA SHA, VLASAK JOSEF: "Separation of post-translational modifications in monoclonal antibodies by exploiting subtle conformational changes under mildly acidic conditions", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 1217, no. 42, 1 October 2010 (2010-10-01), AMSTERDAM, NL, pages 6496 - 6502, XP093088239, ISSN: 0021-9673, DOI: 10.1016/j.chroma.2010.08.044
MASAKI KUROGOCHI, MORI MASAKO, OSUMI KENJI, TOJINO MAMI, SUGAWARA SHU-ICHI, TAKASHIMA SHOU, HIROSE YURIKO, TSUKIMURA WATARU, MIZUN: "Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 10, no. 7, 22 July 2015 (2015-07-22), pages e0132848, XP055239650, DOI: 10.1371/journal.pone.0132848
CHIN-WEI LIN, MING-HUNG TSAI, SHIOU-TING LI, TSUNG-I TSAI, KUO-CHING CHU, YING-CHIH LIU, MENG-YU LAI, CHIA-YU WU, YUNG-CHIEH TSENG: "A common glycan structure on immunoglobulin G for enhancement of effector functions", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 34, 25 August 2015 (2015-08-25), pages 10611 - 10616, XP055425565, ISSN: 0027-8424, DOI: 10.1073/pnas.1513456112
OU CHONG, LI CHAO, ZHANG ROUSHU, YANG QIANG, ZONG GUANGHUI, DAI YUANWEI, FRANCIS REBECCA L., BOURNAZOS STYLIANOS, RAVETCH JEFFREY : "One-Pot Conversion of Free Sialoglycans to Functionalized Glycan Oxazolines and Efficient Synthesis of Homogeneous Antibody–Drug Conjugates through Site-Specific Chemoenzymatic Glycan Remodeling", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 32, no. 8, 18 August 2021 (2021-08-18), US , pages 1888 - 1897, XP093088241, ISSN: 1043-1802, DOI: 10.1021/acs.bioconjchem.1c00314
WANG SHIYI, IONESCU ROXANA, PEEKHAUS NORBERT, LEUNG JIN-YU, HA SHA, VLASAK JOSEF: "Separation of post-translational modifications in monoclonal antibodies by exploiting subtle conformational changes under mildly acidic conditions", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 1217, no. 42, 1 October 2010 (2010-10-01), AMSTERDAM, NL, pages 6496 - 6502, XP093088239, ISSN: 0021-9673, DOI: 10.1016/j.chroma.2010.08.044
MASAKI KUROGOCHI, MORI MASAKO, OSUMI KENJI, TOJINO MAMI, SUGAWARA SHU-ICHI, TAKASHIMA SHOU, HIROSE YURIKO, TSUKIMURA WATARU, MIZUN: "Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 10, no. 7, 22 July 2015 (2015-07-22), pages e0132848, XP055239650, DOI: 10.1371/journal.pone.0132848
CHIN-WEI LIN, MING-HUNG TSAI, SHIOU-TING LI, TSUNG-I TSAI, KUO-CHING CHU, YING-CHIH LIU, MENG-YU LAI, CHIA-YU WU, YUNG-CHIEH TSENG: "A common glycan structure on immunoglobulin G for enhancement of effector functions", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 34, 25 August 2015 (2015-08-25), pages 10611 - 10616, XP055425565, ISSN: 0027-8424, DOI: 10.1073/pnas.1513456112
OU CHONG, LI CHAO, ZHANG ROUSHU, YANG QIANG, ZONG GUANGHUI, DAI YUANWEI, FRANCIS REBECCA L., BOURNAZOS STYLIANOS, RAVETCH JEFFREY : "One-Pot Conversion of Free Sialoglycans to Functionalized Glycan Oxazolines and Efficient Synthesis of Homogeneous Antibody–Drug Conjugates through Site-Specific Chemoenzymatic Glycan Remodeling", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 32, no. 8, 18 August 2021 (2021-08-18), US , pages 1888 - 1897, XP093088241, ISSN: 1043-1802, DOI: 10.1021/acs.bioconjchem.1c00314
Attorney, Agent or Firm:
KAWAGUTI & PARTNERS (JP)
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