Title:
METHOD FOR SYNTHESIS OF SUPPRESSOR tRNA, DNA CONSTRUCT, AND PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE DNA CONSTRUCT
Document Type and Number:
WIPO Patent Application WO/2007/099854
Kind Code:
A1
Abstract:
Disclosed is a DNA construct comprising: a non-eukaryotic suppressor tRNA gene which contains no internal promoter sequence capable of functioning in an eukaryotic cell; and an eukaryotic or bacteriophage-derived promoter ligated to the 5'-terminus of the tRNA gene. Also disclosed is a method for synthesis of suppressor tRNA using the DNA construct. Further disclosed is a method for production of a protein having a non-natural amino acid integrated therein by using the DNA construct.
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Inventors:
YOKOYAMA SHIGEYUKI (JP)
SAKAMOTO KENSAKU (JP)
HINO NOBUMASA (JP)
MUKAI TAKAHITO (JP)
KOBAYASHI TAKATSUGU (JP)
SAKAMOTO KENSAKU (JP)
HINO NOBUMASA (JP)
MUKAI TAKAHITO (JP)
KOBAYASHI TAKATSUGU (JP)
Application Number:
PCT/JP2007/053304
Publication Date:
September 07, 2007
Filing Date:
February 22, 2007
Export Citation:
Assignee:
RIKEN (JP)
YOKOYAMA SHIGEYUKI (JP)
SAKAMOTO KENSAKU (JP)
HINO NOBUMASA (JP)
MUKAI TAKAHITO (JP)
KOBAYASHI TAKATSUGU (JP)
YOKOYAMA SHIGEYUKI (JP)
SAKAMOTO KENSAKU (JP)
HINO NOBUMASA (JP)
MUKAI TAKAHITO (JP)
KOBAYASHI TAKATSUGU (JP)
International Classes:
C12N15/79; C12N1/15; C12N1/19; C12N5/10; C12N15/09; C12P21/00
Domestic Patent References:
| WO2004039989A1 | 2004-05-13 |
Other References:
KOBAYASHI T. ET AL.: "Pyrrolysine tRNA Gosei Koso tRNAPY1 pear o Mochiita Dobutsu Saibo ni okeru Idenshi Ango no Kakucho", RNA MEETING, vol. 7TH, 2005, pages 101, XP003016880
BLIGHT S. ET AL.: "Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo", NATURE, vol. 431, 2004, pages 333 - 335, XP003007828
DOMITROVICH A.M. AND KUNKEL G.R.: "Multipl dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies", NUCLEIC ACIDS RES., vol. 31, no. 9, 2003, pages 2344 - 2352, XP003016881
MURPHY J.T. ET AL.: "Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition", J. BIOL. CHEM., vol. 262, no. 4, 1987, pages 1795 - 1803, XP003016882
ILEGEMS E. ET AL.: "Downregulation of eRF1 by RNA interference increases mis-acylated tRNA suppression efficiency in human cells", PROTEIN ENG. DES. SEL., vol. 17, no. 12, 2004, pages 821 - 827, XP003016883
See also references of EP 1992698A4
BLIGHT S. ET AL.: "Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo", NATURE, vol. 431, 2004, pages 333 - 335, XP003007828
DOMITROVICH A.M. AND KUNKEL G.R.: "Multipl dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies", NUCLEIC ACIDS RES., vol. 31, no. 9, 2003, pages 2344 - 2352, XP003016881
MURPHY J.T. ET AL.: "Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition", J. BIOL. CHEM., vol. 262, no. 4, 1987, pages 1795 - 1803, XP003016882
ILEGEMS E. ET AL.: "Downregulation of eRF1 by RNA interference increases mis-acylated tRNA suppression efficiency in human cells", PROTEIN ENG. DES. SEL., vol. 17, no. 12, 2004, pages 821 - 827, XP003016883
See also references of EP 1992698A4
Attorney, Agent or Firm:
KATO, Asamichi et al. (daVinci BOSEI 7th Floor 20-12 Shin-Yokohama 3-chome, Kohoku-ku, Yokohama-sh, Kanagawa 33, JP)
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