Title:
MODIFIED Cas9 PROTEINS AND APPLICATION FOR SAME
Document Type and Number:
WIPO Patent Application WO/2019/026976
Kind Code:
A1
Abstract:
Modified CjCas9 proteins that can better bind to guide RNA and are useful as tools for gene editing. For example, a protein that can bind to guide RNA and comprises a sequence that includes the amino acid sequence represented by SEQ ID NO: 2 but has arginine instead of glutamic acid at position 946, arginine instead of alanine at position 911, and arginine instead of isoleucine at position 920.
Inventors:
NUREKI OSAMU (JP)
NISHIMASU HIROSHI (JP)
YAMADA MARI (JP)
YAMAGATA TETSUYA (US)
QIN YUANBO (US)
NISHIMASU HIROSHI (JP)
YAMADA MARI (JP)
YAMAGATA TETSUYA (US)
QIN YUANBO (US)
Application Number:
PCT/JP2018/028926
Publication Date:
February 07, 2019
Filing Date:
August 01, 2018
Export Citation:
Assignee:
UNIV TOKYO (JP)
EDIGENE CORP (JP)
EDIGENE CORP (JP)
International Classes:
C12N15/09; C12N9/22; C12N15/55
Domestic Patent References:
| WO2016021973A1 | 2016-02-11 |
Foreign References:
| JP2018143208A | 2018-09-20 |
Other References:
DATABASE UniprotKB/TrEMBL [o] 27 May 2015 (2015-05-27), ANONYMOUS: "CRISPR-associated endonuclease Cas9/Csn1", XP055575337, retrieved from Uniprot Database accession no. AOAOD7V4T2
DATABASE GenBank [O] 4 March 2015 (2015-03-04), "Campylobacter jejuni subsp. jejuni strain C12-5524 contig019, whole genome shotgunsequence", XP055573079, Database accession no. JYDZ01000019
DATABASE UniprotKB/TrEMBL [o] 5 July 2017 (2017-07-05), ANONYMOUS: "CRISPR-associated endonuclease Cas9", XP055575341, retrieved from Uniprot Database accession no. D2MWB9
DATABASE Nucleotide [O] 12 January 2010 (2010-01-12), "Campylobacter jejuni subsp. jejuni 414 contig00002, whole genome shotgun sequence", XP055573088, retrieved from NCBI Database accession no. ADGM01000002
YAMADA, M. ET AL.: "Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems", MOL. CELL, vol. 65, no. 6, March 2017 (2017-03-01), pages 1109 - 1121, XP029959270
SPENCER, JM. ET AL.: "Characterizing the Mutational Limits of S. pyogenes Cas9", MOL. THER., vol. 25, no. 5, May 2017 (2017-05-01)
DATABASE GenBank [O] 4 March 2015 (2015-03-04), "Campylobacter jejuni subsp. jejuni strain C12-5524 contig019, whole genome shotgunsequence", XP055573079, Database accession no. JYDZ01000019
DATABASE UniprotKB/TrEMBL [o] 5 July 2017 (2017-07-05), ANONYMOUS: "CRISPR-associated endonuclease Cas9", XP055575341, retrieved from Uniprot Database accession no. D2MWB9
DATABASE Nucleotide [O] 12 January 2010 (2010-01-12), "Campylobacter jejuni subsp. jejuni 414 contig00002, whole genome shotgun sequence", XP055573088, retrieved from NCBI Database accession no. ADGM01000002
YAMADA, M. ET AL.: "Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems", MOL. CELL, vol. 65, no. 6, March 2017 (2017-03-01), pages 1109 - 1121, XP029959270
SPENCER, JM. ET AL.: "Characterizing the Mutational Limits of S. pyogenes Cas9", MOL. THER., vol. 25, no. 5, May 2017 (2017-05-01)
Attorney, Agent or Firm:
TAKASHIMA, Hajime et al. (JP)
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