MACROCYCLIC HIV-1 PROTEASE INHIBITORS AND USES THEREOF

BACKGROUND OF THE INVENTION

[0001] HIV protease inhibitor-based active antiretroviral therapy (ART) has been proven to be most effective treatment regimens for HIV/AIDS. However, drug-side effects and emergence of drug-resistance are making ART far less effective or ineffective outright,

SUMMARY

[0002] The inventors have designed and developed a series of macrocyclic HIV-1 protease Inhibitors based upon X-ray structure of an inhibitor- bound HIV-1 protease. The macrocyclic inhibitors of the various embodiments described herein are capable of forming extensive hydrogen bonding interactions with the HIV-1 protease enzyme active-site backbone, an important concept behind the development of compounds against resisted HIV. While not wishing to be bound by any specific theory, it is believed that Inhibitors that contain a flexible macrocycie involving allow effective repacking at the mutation site. It is believed that this factor may influence the enzyme-inhibitory potency and antiviral potency of the compound of the various embodiments described herein. In addition, a number of the compounds of the various embodiments described herein have been shown to maintain potency against multi-drug- resistant HIV variants. This class of inhibitors may exhibit improved pharmacological properties compared to darunavlr and other FDA approved- Inhibitors.

DETAILED DESCRIPTION OF THE INVENTION

[0003] Reference will now be made in detail to certain examples of the disclosed subject matter. While the disclosed subject matter will be described in conjunction with the enumerated claims, it will be understood that the exemplified subject matter is not intended to limit the claims to the disclosed subject matter.

[QQ04] Various examples described herein are directed to a compound of the formula (!):

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

R ¹ and R ² are each independently hydrogen, alky! or ary!alky! ;

R ³ and R ⁴ are each Independently H, alky! or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalkyl or a heterocyc!yl group;

Y ¹ is optionally substituted alkylene (e.g., CH ₂ ), O, or N-R ^a , wherein R ^a Is hydrogen or aikyl ;

X ¹ and X ² are each independently oxygen, S, S(O), S0 ₂ , optionally substituted nitrogen, or optionally substituted alkylene;

each R ⁵ is independently hydrogen, -OR ^b , wherein R ^b is aikyl or aryl, -SQ2R ⁷ , -NR ⁷ 2, -CHR OR ⁷ or CR ⁷ 3, wherein each R ⁷ Is Independently hydrogen , aikyl, heteroaikyl or heterocyclyl or two adjacent R ⁵ groups, together with the carbon atoms to which they are attached, form a heteroaryl group;

each R ⁶ is independently hydrogen, halo (e.g., mono-fluoro and di-fluoro), -NR ⁷ 2, aikyl, aryl, alky!aryl, heteroaikyl or heterocyclyl ;

y is an integer from 1 to 3; (e.g., 1 ); p is an integer from 1 to 3 (e.g. , 1 or 2); and

n is an integer from 0-4 (e.g., an integer from 1 -4).

[0005] Various examples described herein are directed to a compound of the formula (l

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

the dashed line represents an E- or Z- double bond;

1 is:

R ¹ and R ² are each independently hydrogen, alkyl or arylalkyl ;

R ³ and R ⁴ are each Independently H, alkyl or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalky! or a heterocyclyl group;

Y ¹ is optionally substituted alky!ene (e.g., CH ₂ ), O, or N-R ^a , wherein R ^a Is hydrogen or alkyl ;

X ^! and X ² are each independently oxygen, S, S(O), SC¾ optionally substituted nitrogen, or optionally substituted a!ky!ene;

each R ⁶ is independently hydrogen, halo (e.g., mono-fluoro and di-f!uoro), -NR ⁷ 2, alkyl, ary!, alkylaryl, heteroa!ky! or heterocyclyl ; R ¹⁰ Is -OR ⁹ or ~NR ⁹ 2, wherein each R ⁹ is independently hydrogen, a!ky!, cycloaiky!, heteroa!kyl or heterocyciy! ;

Y ² and Y ³ are each independently NH, S, O, NR ⁹ or CR ^{1 1} 2, wherein each R ^{1 1} Is independently hydrogen, alkyl , aryi, or heterocyclyl ;

p is an integer from 1 to 3 (e.g. , 1 or 2); and

n is an integer from 0-4 (e.g., an Integer from 1 -4).

[0006] In some embodiments, the compounds of the formula (I) and (ia) are compounds

respectively.

[0007] Various other examples described herein are directed to compounds of the for

(lb)

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

n E- or Z- double bond;

R ¹ and R ² are each independently hydrogen, alky! or arylalkyl ;

R ³ and R ⁴ are each Independently H, alky! or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalkyl or a heterocyc!yl group;

X ^! and X ² are each independently oxygen, S, S(O), S0 ₂ , optionally substituted nitrogen, or optiona!!y substituted a!ky!ene;

R ⁵ Is hydrogen, -GR ^b , wherein R ^b is aikyi or aryi, -SQ ₂ R ⁷ , -NR ⁷ ₂ , -CHROR ⁷ or CR ⁷ 3, wherein each R ⁷ is independently hydrogen, aikyi, heteroalkyi or heterocyclyl or two adjacent R ⁵ groups, together with the carbon atoms to which they are attached, form a heteroaryl group;

each R ⁶ is independently hydrogen, halo (e.g., mono-fluoro and di-fluoro), -NR ⁷ 2, aikyi, aryi, alkyiaryl, heteroalkyi or heterocyciyl ;

p is an integer from 1 to 3 (e.g. , 1 or 2); and

n is an integer from 0-4 (e.g., an integer from 1 -4).

[0008] In some embodiments of the compounds of the formula (lb), the rmula:

[0009] Various examples described herein are directed to a compound of the formula (II):

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

wherein each R ⁹ is Independently hydrogen, a!kyl, heieroalky! or heterocyc!yl ; R ¹ and R ² are each independently hydrogen, alky! or ary!alkyl ;

R ³ and R ⁴ are each Independently H, alky! or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalkyl or a heterocyc!yl group;

X ¹ and X ² are each independently oxygen, S, S(O), S0 ₂ , optionally substituted nitrogen, or optionally substituted a!ky!ene;

each R ⁵ is independently hydrogen, -OR ^b , wherein R ^b is aikyl or ary! , -S0 ₂ R ⁷ , -

NR ⁷ 2, -CHR' R ⁷ or CR ⁷ 3, wherein each R ⁷ is independently hydrogen, aikyl, heteroa!kyl or heterocyc!yl or two adjacent R ⁵ groups, together with the carbon atoms to which they are attached, form a heteroaryi group;

each R ⁶ is independently hydrogen, halo, -NR ⁷ ₂ , aikyl, aryl, aiky!ary! , heieroalky! or heterocyc!y! ;

y is an integer from 1 to 3; (e.g., 1 );

p is an integer from 1 to 3 (e.g. , 1 or 2); and

n is an integer from 0-4 (e.g., an integer from 1 -4).

[0010] In some embodiments of the compounds of the formula (II), the group Z ² is a group of the formula:

-OR ⁹ or -NR ⁹ 2, wherein each R ⁹ is independently hydrogen, a!kyi, heteroaiky! or heterocycly! .

[0011] Examples of compounds encompassed by the formulae (I), (la), (ib), and (II) include:

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PCT/US2018/040342





[QQ12] Those of ordinary skili in the art will recognize that compounds described herein can contain chira! centers. Ail diastereomers of the compounds described herein are contemplated herein, as well as racemates.

[0013] Various examples of the present Invention also contemplate pharmaceutical compositions comprising one or more compounds of the various examples of the present invention (e.g., compounds of the formulae (I), (la), (lb), and (II)) and one or more pharmaceutically acceptable carriers, diluents, excipients or combinations thereof. A "pharmaceutical composition" refers to a chemical or biological composition suitable for administration to a subject (e.g., mammal). Such compositions may be specifically formuiated for administration via one or more of a number of routes, including but not limited to buccal, cutaneous, epicutaneous, epidural, infusion, inhalation , Intraarterial, intracardial, intracerebroventricular, intradermal, intramuscular, intranasal , intraocular, intraperitoneal, Intraspinal , Intrathecal, intravenous, oral, parenteral, pulmonary, recta!!y via an enema or suppository, subcutaneous, subdermal, sublingual, transdermal , and iransmucosa!. In addition, administration can by means of capsule, drops, foams, gel, gum, injection, liquid, patch, pill, porous pouch, powder, tablet, or other suitable means of administration.

[0014] A "pharmaceutical excipient" or a "pharmaceutically acceptable excipient" comprises a carrier, sometimes a liquid, in which an active therapeutic agent is formulated. The excipient generally does not provide any pharmacological activity to the formulation, though it may provide chemical and/or biological stability, and release characteristics. Examples of suitable formulations can be found, for example, in Remington, The Science And Practice of Pharmacy, 20th Edition, (Gennaro, A. R,, Chief Editor), Philadelphia College of Pharmacy and Science, 2000, which is incorporated by reference in its entirety.

[0015] As used herein "pharmaceutically acceptable carrier" or

"excipienf Includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents that are physiologically compatible. In one example, the carrier is suitable for parenteral administration. Alternatively, the carrier can be suitable for intravenous, Intraperitoneal, intramuscular, sublingual, or oral administration. Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of steriie injectable solutions or dispersion. The use of such media and agents for pharmaceutically active substances Is well known in the art. Except Insofar as any conventional media or agent is Incompatible with the active compound, use thereof in the pharmaceutical compositions of the invention Is contemplated. Supplementary active compounds can also be incorporated into the compositions.

[0016] Pharmaceutical compositions may be steriie and stable under the conditions of manufacture and storage. The composition can be formulated as a solution, microemulsion, liposome, or other ordered structure suitable to high drug concentration. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol, and liquid polyethylene glycol), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.

[0017] In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannltol, sorbitol, or sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including In the composition an agent which delays absorption, for example, monostearate salts and gelatin. Moreover, the compounds described herein can be formulated in a time release formulation, for example in a composition that includes a slow release polymer. The active compounds can be prepared with carriers that will protect the compound against rapid release, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers may be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, poiyorihoesters, po!ylactic acid and polyiaciic, polyglycolic copolymers (PLG). Many methods for the preparation of such formulations are known to those skilled in the art.

[0018] Oral forms of administration are also contemplated herein. The pharmaceutical compositions of the present Invention may be orally administered as a capsule (hard or soft), tablet (film coated, enteric coated or uncoated), powder or granules (coated or uncoated) or liquid (solution or suspension). The formulations may be conveniently prepared by any of the methods well-known in the art. The pharmaceutical compositions of the present invention may Include one or more suitable production aids or excipients including fillers, binders, disintegrants, lubricants, diluents, flow agents, buffering agents, moistening agents, preservatives, colorants, sweeteners, flavors, and pharmaceutically compatible carriers.

[0019] For each of the recited examples, the compounds can be administered by a variety of dosage forms as known in the art. Any biologically- acceptable dosage form known to persons of ordinary skill in the art, and combinations thereof, are contemplated. Examples of such dosage forms Include, without limitation, chewable tablets, quick dissolve tablets, effervescent tablets, reconstitutable powders, elixirs, liquids, solutions, suspensions, emulsions, tablets, multi-layer tablets, bi-layer tablets, capsules, soft gelatin capsules, hard gelatin capsules, caplets, lozenges, chewable lozenges, beads, powders, gum, granules, particles, microparticles, dispersible granules, cachets, douches, suppositories, creams, topicais, inhalants, aerosol inhalants, patches, particle inhalants, implants, depot implants, ingestibles, injectables (including subcutaneous, intramuscular, Intravenous, and intradermal), infusions, and combinations thereof.

[0020] Other compounds which can be included by admixture are, for example, medically inert ingredients (e.g., solid and liquid diluent), such as lactose, dextrosesaccharose, cellulose, starch or calcium phosphate for tablets or capsules, olive oil or ethyl oleate for soft capsules and water or vegetable oil for suspensions or emulsions; lubricating agents such as silica, talc, stearic acid, magnesium or calcium stearate and/or polyethylene glycols; gelling agents such as colloidal clays; thickening agents such as gum tragacanth or sodium alginate, binding agents such as starches, arabic gums, gelatin, methylcelluiose, carboxymethylcellulose or polyvinylpyrrolidone; disintegrating agents such as starch, aiginic acid, alginates or sodium starch glycoiate; effervescing mixtures; dyestuff; sweeteners; wetting agents such as lecithin, poiysorbates or !aurylsu!phates; and other therapeutically acceptable accessory ingredients, such as humectants, preservatives, buffers and antioxidants, which are known additives for such formulations.

[0021] Liquid dispersions for oral administration can be syrups, emulsions, solutions, or suspensions. The syrups can contain as a carrier, for example, saccharose or saccharose with glycerol and/or mannito! and/or sorbitol. The suspensions and the emulsions can contain a carrier, for example a natural gum, agar, sodium alginate, pectin, methy!cei!u!ose, carboxymethyiceiluiose, or polyvinyl alcohol.

[0022] The amount of active compound in a therapeutic composition according to various examples of the present invention may vary according to factors such as the disease state, age, gender, weight, patient history, risk factors, predisposition to disease, administration route, pre-existing treatment regime (e.g., possible Interactions with other medications), and weight of the individual . Dosage regimens may be adjusted to provide the optimum therapeutic response. For example, a single bolus may be administered, several divided doses may be administered over time, or the dose may be proportionally reduced or Increased as indicated by the exigencies of therapeutic situation.

[0023] "Dosage unit form," as used herein, refers to physically discrete units suited as unitary dosages for the mammalian subjects to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the Invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals. In therapeutic use for treatment of conditions in mammals (e.g., humans) for which the compounds of the present Invention or an appropriate pharmaceutical composition thereof are effective, the compounds of the present invention may be administered in an effective amount. The dosages as suitable for this invention may be a composition, a pharmaceutical composition or any other compositions described herein.

[0024] For each of the recited examples, the dosage is typically administered once, twice, or thrice a day, although more frequent dosing Intervals are possible. The dosage may be administered every day, every 2 days, every 3 days, every 4 days, every 5 days, every 6 days, and/or every 7 days (once a week). In one example, the dosage may be administered daily for up to and including 30 days, preferably between 7-10 days. In another example, the dosage may be administered twice a day for 10 days. If the patient requires treatment for a chronic disease or condition, the dosage may be administered for as long as signs and/or symptoms persist. The patient may require "maintenance treatment" where the patient is receiving dosages every day for months, years, or the remainder of their lives. In addition , the composition of this invention may be to effect prophylaxis of recurring symptoms. For example, the dosage may be administered once or twice a day to prevent the onset of symptoms in patients at risk, especially for asymptomatic patients.

[0025] The compositions described herein may be administered in any of the following routes: buccal, epicutaneous, epidural, infusion , inhalation, intraarterial, intracardiai, intracerebroventricular, intradermal, intramuscular, intranasal, intraocular, intraperitoneal, intraspinal, intrathecal, intravenous, oral, parenteral , pulmonary, rectally via an enema or suppository, subcutaneous, subdermai, sublingual, transdermal, and transmucosai. The preferred routes of administration are buccal and oral. The administration can be local, where the composition is administered directly, close to, in the locality, near, at, about, or In the vicinity of, the site(s) of disease, e.g., Inflammation, or systemic, wherein the composition is given to the patient and passes through the body widely, thereby reaching the slte(s) of disease. Local administration can be administration to the cell, tissue, organ , and/or organ system, which encompasses and/or is affected by the disease, and/or where the disease signs and/or symptoms are active or are likely to occur. Administration can be topical with a local effect, composition is applied directly where its action is desired. Administration can be enteral wherein the desired effect is systemic (non-local), composition is given via the digestive tract. Administration can be parenteral, where the desired effect is systemic, composition Is given by other routes than the digestive tract.

[QQ26] In some examples, the various examples of the present invention contemplate pharmaceutical compositions comprising a therapeutically effective amount of one or more compounds of the various examples of the present Invention. In some aspects, the various examples of the present invention contemplate a compound of the formulae (I), (la), (lb), and (II) for use as a medicament for treating a patient in need of relief from a disease or a condition, such as HIV/AIDS. Other embodiments are directed to a method for treating a patient (e.g., a human patient) In need of relief from HIV/AIDS, the method comprising the step of administering to the patient a therapeutically effective amount of a compound of the formula (I), (la), (lb) or (II) or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the formula (I), (la), (lb) or (I I).

[0027] The term "therapeutically effective amount" as used herein, refers to that amount of one or more compounds of the various examples of the present invention that elicits a biological or medicinal response in a tissue system, animal or human, that is being sought by a researcher, veterinarian, medical doctor or other clinician , which includes alleviation of the symptoms of the disease or disorder being treated. In some examples, the therapeutical iy effective amount is that which may treat or alleviate the disease or symptoms of the disease at a reasonable benefit/risk ratio applicable to any medical treatment. However, it is to be understood that the total daily usage of the compounds and compositions described herein may be decided by the attending physician within the scope of sound medical judgment. The specific therapeuticai!y-effective dose level for any particular patient will depend upon a variety of factors, including the condition being treated and the severity of the condition; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, gender and diet of the patient: the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidentaliy with the specific compound employed; and like factors well known to the researcher, veterinarian, medical doctor or other clinician. It is also appreciated that the therapeutically effective amount can be selected with reference to any toxicity, or other undesirable side effect, that might occur during administration of one or more of the compounds described herein.

[0028] In some examples, the compounds of the various examples of the present invention have a half maximal inhibitory concentration (IGso) of from about 5 nM to about 500 nM (e.g., about 50 nM to about 100 nM, about 10 nM to about 75 nM, about 10 nM to about 60 nM, about 100 nM to about 500 nM, about 50 μΜ to about 250 nM, about 00 nM to about 300 nM or about 10 nM to about 30 nM).

[0029] Values expressed in a range format should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to Include ail the individual numerical values or subranges encompassed within that range as if each numerical value and sub- range were explicitly recited. For example, a range of "about 0.1 % to about 5%" or "about 0.1 % to 5%" should be interpreted to include not just about 0.1 % to about 5%, but also the i ndividual values (e.g., 1 %, 2%, 3%, and 4%) and the sub-ranges (e.g., 0.1 % to 0.5%, 1 .1 % to 2.2%, 3.3% to 4.4%) withi n the i ndicated range. The statement "about X to Y" has the same meaning as "about X to about Y," unless indicated otherwise. Likewise, the statement "about X, Y, or about Z" has the same meaning as "about X, about Y, or about Z," unless i ndicated otherwise.

[0030] !n this document, the terms "a," "an ," or "the" are used to i nclude one or more than one u nless the context clearly dictates otherwise. The term "or is used to refer to a nonexclusive "or" unless otherwise indicated. In addition, it is to be understood that the phraseology or terminology employed herein , and not otherwise defined, is for the pu rpose of description only and not of limitation . Any use of section headings is intended to aid reading of the document and is not to be interpreted as limiting. Further, i nformation that is relevant to a section heading may occur within or outside of that particular section. Furthermore, ail publications, patents, and patent documents referred to in this document are incorporated by reference herein in thei r entirety, as though Individually incorporated by reference.

[0031 ] in the methods described herein, the steps can be carried out in any order without departing from the principles of the invention , except when a temporal or operational sequence is explicitly recited. Furthermore, specified steps can be carried out concurrently unless explicit clai m language recites that they be carried out separately. For example, a clai med step of doing X and a claimed step of doi ng Y can be conducted simu ltaneously within a single operation , and the resulti ng process will fall within the literal scope of the claimed process.

[0032] The term "about" as used herein can al low for a degree of variability in a val ue or range, for example, within 10%, within 5%, or within 1 % of a stated value or of a stated limit of a range.

[QQ33] The term "substituted" and "optionally substituted" as used herein refers to a group (e.g. , aikyl , aryi , and heteroaryl) or molecule In which one or more hydrogen atoms contai ned thereon are replaced by one or more substituents. The term "substituent" as used herein refers to a group that can be or is substituted onto a molecule or onto a group. Examples of substituents I nclude, but are not li mited to, a halogen (e.g., F, CI, Br, and i); an oxygen atom I n groups such as hydroxyl groups, alkoxy groups, aryioxy groups, ara ikyloxy groups, oxo(carbony!) groups, carboxy! groups including carboxy!ic acids, carboxylates, and carboxylate esters; a sulfur atom In groups such as thiol groups, alkyi and aryl sulfide groups, sulfoxide groups, su!fone groups, suifonyi groups, and sulfonamide groups; a nitrogen atom in groups such as amines, hydroxyiamines, nitriies, nitro groups, N-oxides, hydrazides, azides, and enamines; and other heteroatoms in various other groups. Non-limiting examples of substituents that can be bonded to a substituted carbon (or other) atom include F, CI , Br, I, OR, OC(0)N (R) ₂ , CM, NO, N0 ₂ , ON0 ₂ , azido, CF ₃ , OGF3, R, O (oxo), S (thiono), C(O), S(O), methylenedioxy, ethylenedioxy, N(R) ₂ , SR, SOR, SO2R, S0 ₂ N(R)2, S0 ₃ R, G(0)R, C(0)C(0)R, C(0)CH ₂ C(0)R, C(S)R, C(0)0R, 0C(0)R, C(0)N(R) ₂ , OC(0)N(R) ₂ , C(S)N(R) ₂ , (CH ₂ )o. ₂ N (R)C(0)R, (CH ₂ )o- ₂ N(R)N(R) ₂ , N(R)N(R)C(0)R, N(R)N(R)C(0)OR, N(R)N(R)CON(R) ₂ , N(R)S0 ₂ R, N(R)S0 ₂ N(R) ₂ , N(R)C(0)OR, N(R)C(0)R, N(R)C(S)R, N(R)C(0)N(R) ₂ , N(R)C(S)N(R) ₂ , N(COR)COR, N(OR)R, C(=NH)N(R) ₂ , G(0)N(OR)R, or C(=NOR)R, wherein R can be, for example, hydrogen, a!ky!, acy!, cycloa!kyl, aryi, ara!kyi, heterocyc!yi, heieroaryi , or heteroaryialkyi.

[0034] The term "alky!" as used herein refers to substituted or unsubstituted straight chain and branched alky! groups and cycloalkyi groups having from 1 to 40 carbon atoms (C1 -C 0), 1 to about 20 carbon atoms (Ci -C ₂ o), 1 to 12 carbons (C1-C12), 1 to 8 carbon atoms (Ci-Ce), or, in some examples, from 1 to 8 carbon atoms (GrC ₆ ). Examples of straight chain alky! groups include those with from 1 to 8 carbon atoms such as methyl, ethyl, n-propyl , n- butyi, n-pentyl, n-hexyl, n-heptyi, and n-octyl groups. Examples of branched alkyi groups include, but are not limited to, isopropyi, iso-butyi, sec-butyl, t-butyi, neopenty!, isopentyi, and 2,2-dimethylpropyl groups. Representative substituted alkyi groups can be substituted one or more times with any of the groups listed herein, for example, amino, hydroxy, cyano, carboxy, nitro, thio, alkoxy, and halogen groups.

[0035] As used herein, the term "heteroaikyi" includes a chain of atoms that includes both carbon and at least one heteroatom such as, but not limited to, N, O, and S, and is optionally branched, in certain variations, illustrative heteroatoms also include phosphorus, and selenium.

[QQ36] The term "cycloalkyi" as used herein refers to substituted or unsubstituted cyclic alky! groups such as, but not limited to, cyc!opropy!, cyc!obutyi, cyc!openty! , cyc!ohexy!, cycloheptyl, and cyc!oocty! groups, in some examples, the cycloalkyi group can have 3 to about 8-12 ring members, whereas in other examples the number of ring carbon atoms range from 3 to 4, 5, 6, or 7, In some examples, cycioaiky! groups can have 3 to 6 carbon atoms (C ₃ -Ge). Cycioaiky! groups further include poiycyclic cyc!oalky! groups such as, but not limited to, norbornyi, adamanty! , bornyl, camphenyl, isocamphenyl, and carenyl groups, and fused rings such as, but not limited to, decalinyl, and the like,

[0037] The term "acyl" as used herein refers to a group containing a carbonyi moiety wherein the group is bonded via the carbonyl carbon atom. The carbonyi carbon atom is also bonded to another carbon atom, which can be part of a substituted or unsubstituted aikyl, aryl , araikyi cycloaikyl, cycloalkyiaikyl, heterocyclyl , heterocyclyl, heteroaryl , heteroaryia!kyl group or the like, in the special case wherein the carbonyi carbon atom is bonded to a hydrogen, the group is a "formyi" group, an acyl group as the term is defined herein. An acyl group can include 0 to about 12-40, 6-1 0, 1 -5 or 2-5 additional carbon atoms bonded to the carbonyi group. An acryloyi group is an example of an acyl group. An acyl group can also include heteroatoms within the meaning here. A nicotinoyi group (pyridyl-3-carbonyl) is an example of an acyl group within the meaning herein. Other examples include acetyl, benzoyl, phenyiacetyl, pyridylacety!, cinnamoyl, and acryloyi groups and the like. When the group containing the carbon atom that is bonded to the carbonyi carbon atom contains a halogen, the group is termed a "haloacy!" group. An example Is a triil uoroacetyl group.

[0038] The term "aryl" as used herein refers to substituted or unsubstituted cyclic aromatic hydrocarbons that do not contain heteroatoms in the ring. Thus aryl groups include, but are not limited to, phenyl , azuienyi, hepta!eny! , bi phenyl, indacenyl, fluorenyl, phenanthrenyl, triphenylenyl, pyrenyl, naphthacenyl, chrysenyi, biphenyienyi, anthracenyl, and naphthyl groups. In some examples, aryl groups contain about 6 to about 14 carbons (CR-C ) or from 6 to 10 carbon atoms (Ge-Cio) in the ring portions of the groups. Aryl groups can be unsubstituted or substituted, as defined herein. Representative substituted aryl groups can be mono-substituted or substituted more than once, such as, but not limited to, 2-, 3-, 4-, 5-, or 6-substituted phenyl or 2-8 substituted naphthyl groups, which can be substituted with carbon or non- carbon groups such as those listed herein.

[0039] The term "ara!kyl" and "ary!a!kyl" as used herein refers to a!kyl groups as defined herein in which a hydrogen or carbon bond of an aikyl group Is replaced with a bond to an aryl group as defined herein. Representative aralkyl groups Include benzyl and phenyiethyi groups. [0040] The term "heterocyciyi" as used herein refers to substituted or unsubstltuted aromatic and non-aromatic ring compounds containing 3 or more ring members, of which , one or more is a heteroatom such as, but not limited to, N, O, and S. Thus, a heterocyciyi can be a cycioheteroalkyl, or a heteroaryl , or if poiycyciic, any combination thereof. In some examples, heterocyciyi groups include 3 to about 20 ring members, whereas other such groups have 3 to about 1 5 ring members, !n some examples, heterocyciyi groups include heterocyciyi groups that include 3 to 8 carbon atoms (0 ₃ -0 ₈ ), 3 to 6 carbon atoms (C3-C ₆ ), 3 to 5 carbon atoms {C3-C5) , 3 to 4 carbon atoms {C3-C4) or 6 to 8 carbon atoms (Ce-Cs). A heterocyciyi group designated as a C ₂ -heterocyciyi can be a 5-ring with two carbon atoms and three heteroatoms, a 8-ring with two carbon atoms and four heteroatoms and so forth. Likewise a C ₄ -heterocyclyl can be a 5-ring with one heteroatom, a 6-ring with two heteroatoms, and so forth. The number of carbon atoms plus the number of heteroatoms equals the total number of ring atoms. A heterocyciyi ring can also include one or more double bonds. A heteroaryl ring is an example of a heterocyciyi group. The phrase "heterocyciyi group" includes fused ring species including those that include fused aromatic and non-aromatic groups. Representative heterocyciyi groups include, but are not limited to piperldyny!, plperazinyl, morphoilny!, furany!, pyrrolldlnyl, pyridiny!, pyrazinyl, pyrimidinyl, trlazinyi, fhiophenyl, tetrahydrofuranyl, pyrro!yl, oxazoly!, Imidazo!yi, triazyoiy!, tetrazolyl, benzoxazoiiny! , and benzimidazo!lny! groups.

[0041] The term "aikoxy" as used herein refers to an oxygen atom connected to an alkyi group, including a cycioalkyi group, as are defined herein. Examples of linear aikoxy groups include but are not limited to methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, and the like. Examples of branched aikoxy include but are not limited to isopropoxy, sec-butoxy, tert-butoxy, Isopentyloxy, isohexyloxy, and the like. Examples of cyclic aikoxy include but are not limited to cyciopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyioxy, and the like. An aikoxy group can include one to about 12-20 or about 12-40 carbon atoms bonded to the oxygen atom, and can further include double or triple bonds, and can also include heteroatoms. For example, an allylox group Is an aikoxy group within the meaning herein. A methoxyethoxy group is also an aikoxy group within the meaning herein, as is a methylenedioxy group in a context where two adjacent atoms of a structure are substituted therewith.

[0042] The term "amine" as used herein refers to primary, secondary, and tertiary amines having, e.g., the formula N(group) ₃ wherein each group can Independently be H or non-H, such as alkyi, aryl, and the like. Amines include but are not limited to a!kylamlnes, arylamines, ary!a!kylamines; dia!ky!amines, diarylamines, diaralkylamines, heterocyclyl amines and the !ike; and ammonium ions.

[0043] The terms "halo," "halogen," or "halide" group, as used herein, by themselves or as part of another substltuent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.

[0044] As used herein, the term "salts" and "pharmaceutically acceptable salts" refer to derivatives of the disclosed compounds wherein the parent compound Is modified by making acid or base salts thereof. Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic groups such as amines; and alkali or organic salts of acidic groups such as carboxylic acids. Pharmaceutically acceptable salts include the conventional non-toxic salts or the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, and nitric; and the salts prepared from organic acids such as acetic, propionic, succinic, g!ycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, su!fanllic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesu!fonic, ethane disu!fonic, oxalic, and isethionic, and the like.

[0045] Pharmaceutically acceptable salts can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods, in some instances, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanoi, Isopropanol, or acetonitrlle are preferred. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, the disclosure of which is hereby incorporated by reference. [QQ46] The term "solvate" means a compound, or a salt thereof, that further Includes a stoichiometric or non-stoichiometric amount of solvent bound by non-covalent intermolecular forces. Where the solvent Is water, the solvate is a hydrate.

[QQ47] The term "prodrug" means a derivative of a compound that can hydroiyze, oxidize, or otherwise react under biological conditions (In vitro or in vivo) to provide an active compound, particularly a compound of the invention. Examples of prodrugs include, but are not limited to, derivatives and metabolites of a compound of the invention that include biohydrolyzabie moieties such as biohydrolyzabie amides, biohydrolyzabie esters, biohydroiyzabie carbamates, biohydrolyzabie carbonates, biohydrolyzabie ureides, and biohydrolyzabie phosphate analogues. Specific prodrugs of compounds with carboxyl functional groups are the lower alky! esters of the carboxylic acid. The carboxylate esters are conveniently formed by esterlfying any of the carboxylic acid moieties present on the molecule. Prodrugs can typically be prepared using well-known methods, such as those described by Burger's Medicinal Chemistry and Drug Discovery 6th ed. (Donald J. Abraham ed., 2001 , Wiley) and Design and Application of Prodrugs (H. Bundgaard ed., 1 985, Harwood Academic Publishers GmbH).

Examples

[0048] The present invention can be better understood by reference to the following examples which are offered by way of illustration . The present invention is not limited to the examples given herein.

[0049] Preparation of suifony! chloride 40 was carried out from commercially available 3-a!ly!oxymethyianisole 39 by treatment with ch!orosulfuric acid at 0 °C followed by reaction of the resulting sulfonic acid with cyanuric chloride in dry acetone in the presence of triethylamine to provide suifonyl chloride 40 in 37% yield (Scheme 1 ). For the synthesis of benzyl ether derivative with 4-amino substitution, the corresponding suifony! chloride derivative 42 was prepared from commercially available 2-chioro-5-nitrobenzyl alcohol 41. Reaction of alcohol with NaH in the presence of TBSCi in dry THF provided the TBS ether. This was converted to the corresponding th!ophenol derivative by reaction with sodium disulfide, freshly prepared from sodium sulfide and elemental sulfur in ethano!ic solution in the presence of NaOH to provide a mixture of the corresponding thiol along with its oxidized disulfide derivative. Oxidation of this mixture by a combination of /V-chiorosuccinimlde and dilute hydrochloric acid in MeCN afforded the corresponding suifonyl chloride 42 in moderate yield.

[QQ50] Commercially available 5-hexenyiamine 43 was reacted with chira! epoxide 44 providing epoxide opening product 45 (Scheme 2). Reaction of these amines with suifonyl chloride derivatives 40 and 42 afforded the corresponding dlene sulfonamide derivatives 46 and 47. For the synthesis of diene 47, sulfonamide intermediate derived from suifonyl chloride 42 was subjected to nRu ₄ N ⁺ P in THF. The resulting alcohol was subjected to O aiiyiation later on with a!iy!-ferf-buiyicarbonaie in the presence of catalytic Pd(PPh to provide 47, For the synthesis of the saturated inhibitors with p- OMe and nitrosuifonamides, diene derivatives 46 and 47 were exposed to ring ciosing olefin metathesis to provide the corresponding unsaturated macrocvcies as E/Z mixtures (approximately 1 :2 E/Z ratio). Deprotectlon of Boc-group with TFA was followed by reaction of the resulted amines 48 and 49 .

[0051] Reaction of amine 48 with activated bis-THF derivative 50 furnished unsaturated inhibitor 51 as a mixture of £/Z isomers (Scheme 3). The mixture of isomers were separated by HPLC using a C18 column to furnish macrocyclic inhibitors. Reaction of amine 49 with a p-N0 ₂ group was converted to E/Z mixture of unsaturated macrocyclic inhibitors 52. Catalytic hydrogenation of 52 over 10% Pd-C provided saturated Inhibitor 7. Similarly, reaction of amine 49 with activated carbonate 53 provided the corresponding carbamate which upon catalytic hydr

[0052] Scheme 1. Reagents and conditions: (a) HS0 ₃ CI, CH ₂ Gi ₂₎ Q °C, 20 min;

(b) cyanuric chloride, EfeN, dry acetone, 23 °C to 60 °C, 24 h, 19% over 2 steps;

(c) TBSCi, NaH (60% susp. In mineral oil), TBAI, dry THF, 0 °C, 1 h, 90%; (d) Na ₂ S ₂ in EtOH, NaOH, EtOH, reflux, 2 h; (e) NCS, 2M HCi In MeCN, -10 °C to 20 °C, 30 min, 35%.

49, R = NO,

[0053] Scheme 2. Reagents and conditions: (a) /PrOH, 56 °C, 14 h, 82%; (b) 40 or 42, aqueous NaHC<¾, CH ₂ CI _2j 23 °C, 1 8 h, 80-92%; (c) TBAF, dry THF, 0 °C, 15 min, 78%; (d) aiiy!-terf-butyicarbonate, Pd(PPh ₃ ) ₄ , dry THF, 60 °C, 3 h, 72%; (e) Grubbs I, dry CH ₂ Ci ₂ , 40 °C, 3-6 h, 92-33%; (f) TFA, CH ₂ Ci ₂ , 23 °C, 3-

[0054] Scheme 3. Reagents and conditions: (a) DIPEA, MeCN, 23 °C, 8 days, 44-49%; (b) H ₂ , 10% Pd/C, EtOAc, 23 °C, 9-12 h, 80-91 %. [0055] Ch!orosu!ionlc acid (0.045 mL, 0.678 mmol) was dissolved In anhydrous CH C! (3 ml.) and the solution was cooled to 0 °C and added to a cooled solution of commercially available 1 -((ailyloxy)methy!)-3-methoxybenzene 39 ( 0 mg, 0.617 mmol) in anhydrous CH2CI2 (2 mL). The reaction mixture was stirred at 0 ^C C for 20 min (TLC monitoring). Solvents were evaporated and the residue was purified by silica gel flash chromatography (10-1 5% MeOH in ΟΗ ₂ 0! ₂ ). The resulting mixture of sulfonic acid isomers 2-((aiiyioxy)methyl)-4- methoxybenzenesulfonic acid and 4-((ailyioxy)methyi)-2- methoxybenzenesuifonic acid (40 mg, 0.155 mmoi) was dissolved in dry acetone (8 mL); irlethyiamine (22 pL, 0.158 mmol) and cyanuric chloride (29 mg, 0.157 mmol) were sequentially added. The reaction mixture was heated to 60 °C and stirred for 24 h. After reaction completion the mixture was allowed to cool to 25 °C and concentrated. The residue was dissolved In EtOAc and filtered through a ceiite p!ug. The fiitrate was concentrated and purified by silica gel f!ash chromatography (15-20 % EtOAc In n-Hexane) to afford 40 (34 mg, 19 % over two steps).

2-[((feff-Buty dimethySsi!yi)oxy)iTiethy!]-4~!iitrober!zenesu!foriy chloride (42).

[0058] Commercially available (2-chloro-5-nitrophenyl)methanol 41 (99 mg, 0.528 mmol) was dissolved in dry THF (5 mL) and the resulting solution was cooled to 0 °C. Sodium hydride (60% suspension in mineral oil, 53 mg, 1 .33 mmol), TBSCI (239 mg, 1 .58 mmol) and TBAI (19 mg, 0.052) were sequentially added at 0 °C and the reaction mixture was stirred for 1 h at 0°C. Reaction mixture was quenched with water and extracted with EtOAc. The combined organic layers were dried on anhydrous Na ₂ SG ₄ , filtered, and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography (10 % EtOAc in n-Hexane) to afford tert-butyi((2-chioro-5- nitrobenzy!)oxy)dimethy!si!ane (143 mg, 90%).

[0057] A solution of Na ₂ S ₂ was freshly prepared by suspending Na ₂ S 9H ₂ 0 ( 04 mg, 0.431 mmol) and elemental sulfur (14 mg, 0.431 mmol) in EtOH (10 mL). The yellow mixture was heated to 30 °C until complete dissolution. tert-Butyl((2- ch!oro-5-nitrobenzy!)oxy)dimethylsilane (99 mg, 0.431 mmol) was dissolved in EtOH (10 mL) and the solution was heated to reflux and the Na ₂ S ₂ solution was slowly added. The reaction mixture was refluxed for 2 h, then allowed to cool to 25 ^C 'C and poured over ice. After Ice melting, the solution was acidified with 2N HCi to pH = 2 and then extracted twice with EtOAc. The combined organic layers were dried on anhydrous Na ₂ S04, filtered, and concentrated under reduced pressure. The crude product was purified by siiica gel column chromatography (10-15% EtOAc in n-Hexane) providing 2-(((tert- butyldlmethylsiiyi)oxy)methyi)-4-nlt!Obenzenethiol along with its oxidized disulfide derivative 1 ,2-bis(2-(((tert-butyidimethylsiiyl)oxy)methyl)-4- nitrophenyijdisuifane. This latter mixture (153 mg, 0.51 1 mmol) was suspended In MeCN (1 .5 mL) and added dropwlse to a cooled (- 10 °C) solution of NCS (273 mg, 2.04 mmol) dissolved In a 2N solution of HCI in MeCN (0.82 mL). The reaction mixture was then allowed to warm to 20 °C and stirred for 30 min. The reaction mixture was treated with diisopropyl ether and washed with brine. The organic layer was dried on anhydrous Na ₂ S04, filtered, and concentrated under reduced pressure to provide 42 (66 mg, 35%), carried on to the next step without further purification. ferf-Buty! ((2S,3/¾-4-( ex-5-er!-1~y1amino)~3-hydroxy~1-phe!iy butar!-2~ y carbamate (45).

[0058] Commercially available hex-5-en-1 -amine 43 (40 mg, 0.403 mmol), was dissolved in PrOH (2 mL) and known iert-butyl ((S)-1 -((S)-oxiran-2-yl)-2- phenyieihyi)carbamate 44 (106 mg, 0.403 mmol) was added. The reaction mixture was heated to 56 °C and stirred for 14 h. After reaction completion solvent was evaporated and the crude product was purified by silica gel column chromatography (5% MeOH (1 % NH ₃ ) in CH ₂ C! ₂ ) providing 45 (1 19 mg, 82%). fen-Butyl ((2S,3l?)~4-((2-((a !y oxy)methyl5-A?-( ex-5~eri~1-yS)-4- methoxypheriy!)suSforiamido)~3- ydroxy~1-p er!y butan-2-y )carbamate (46).

[0059] A solution of su!fonyl chloride 40 (33 mg, 0.1 9 mmol) In CH ₂ CI ₂ (3 mL) was added to a round bottom flask containing amine 45 (47 mg, 0.131 mmol). The resulting biphasic mixture was stirred vigorously at 23 °C for 18 h, then the layers were allowed to separate. The organic layer was dried on anhydrous Na ₂ SQ ₄ , filtered, and concentrated under reduced pressure. The crude product was puriiied by silica gel column chromatography (50% EtOAc in n-Hexane) providing 46 (66 mg, 92%),

ferf-Butyl ((2S,3fl)-4-((2-((allyloxy)methyl)-W-(hex-5-en-1-yl)-4- ri!tropheriy su!foiiarr!sdo)-3- yc roxy-1 -pheoyfcutan~2-y carbamate (47).

[0060] A solution of sulfonyl chloride 42 (34 mg, 0,093 mmol) in CH ₂ CI ₂ (3 mL) was added to a round bottom flask containing amine 45 (28 mg, 0.077 mmol). The resulting biphasic mixture was stirred vigorously at 23 °C for 18 h, then the layers were allowed to separate. The organic layer was dried on anhydrous Na ₂ S04, filtered, and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography (40% EtOAc in n-Hexane) providing tert-butyi ((2S,3R)-4-((2-(((tert-butyldimethylsliyl)oxy)methyi)-N-(hex -5- en-1 -yl)-4-nitrophenyl)sulfonamido)-3-hydroxy-1 -phenylbutan-2-yl)carbamate (42 mg, 80%).

[0061] This latter (36 mg, 0.052 mmol) was dissolved in dry THF (2 mL) and the solution cooled to 0 °C. TBAF (1 M in THF, 57 μΐ, 0.057 mmol) was added and the reaction mixture was stirred at 0 °C for 15 min then quenched with saturated aqueous NH ₄ CI and extracted with EtOAc. The crude product was purified by silica gel column chromatography (30% EtOAc in n-Hexane) providing tert-butyl i(2S,3R)-4-((N-(hex-5-en-1 -yl)-2-(hydroxymethyl)-4-nitrophenyi)sulfonamido)-3- hydroxy-1 -phenylbiitan-2-yl)carbamate (23 mg, 78%). This latter (21 mg, 0.036 mmol), was added to a flame dried round bottom flask. Pd(PPh ₃ )4 (4.2 mg, 0.0036 mmo!, 10 mo!%) and a!!y!-tert-buty!carbonate (7 mg, 0.044 mmo!) followed by dry THF (5 ml.) were added and the reaction mixture was heated to 60 °C for 3 h. The mixture was then concentrated and the residue was purified by silica gel column chromatography (20% EtOAc in n-Hexane) providing 47 (16 mg, 72%).

2-((2R,3S)-3-Amino-2~ ydroxy~4-phenyIbutyt)-13~methoxy-2,3 ₎ 4 _i 5,6 _! 9~ hexahydro-11 H~benzo[c][1 ]oxa[5]t ia [6]azacycSotridecine 1 ,1 -dioxide (48).

[0062] Compound 46 (64 mg, 0.106 mmoi) was dissolved in dry CH ₂ CI ₂ (40 mL) and Grubbs I catalyst (9 mg, 0.0109 mmol, 10 mol %) was added to the solution. The reaction mixture was stirred at 40 ^C C for 4 h and then quenched by adding ethyl vinyl ether and concentrated. The crude product was purified by silica gel column chromatography (25% EiOAc in n-Hexane) providing tert-butyl ((2S,3R)-3-hydroxy-4-(13-methoxy-1 ,1 -dioxido-4,5,6,9-tetrahydro-1 1 H- benzo[c][1 ]oxa[5]thia[6] azacyciotridecin-2(3H)-yi)-1 -phenylbutan-2- yi)carbamate as E/Z mixture (57 mg, 93%). This latter (10 mg, 0.0173 mmol) was dissolved in CH ₂ CI ₂ (3 mL) and the solution was cooled to 0 °C. TEA (200 μ!_) was added and the reaction mixture was stirred at 25 °C for 3 h. Solvents were evaporated and the residue was taken up with CH ₂ CI ₂ (12 ml..), treated with 2 ml. of saturated aqueous NaHC(¾, dried on anhydrous Na ₂ SCXs, filtered, and concentrated under reduced pressure. The resulting 2-((2R,3S)-3-amino-2- hydroxy-4-phenylbutyl)-13-methoxy-2,3,4,5,6,9-hexahydro-1 1 H- benzo[c][1 ]oxa[5]thia [6jazacyciotrldeclne 1 ,1 -dioxide (48) was submitted to the next step without further purification.

(3l?,3aS,6a^-Hexahydrofuro[2,3-&lfurar!-3~y ((2S,3 ?)~3-hydroxy-4-(13- met oxy-1 -draxiidQ-4,5,6,9-tetrahydro~11 H- benzo[c][1loxa[5]thia[6]azacyctotridecir!-2(3H)-y!)-1 ~p ersy!foutar!~2- y )carbamate (51 ).

[0063] Amine 48 (8 mg, 0.0173 mmoi) was dissolved in MeCN (2 mL) and N,N- DIPEA (30 μί, 0.173 mmol) followed by known activated b/s-THF derivative 50 (5 mg 0.0 70 mmol) were added. The reaction mixture was stirred at 25 °C for 8 days, then volatiies were evaporated under reduced pressure and the residue was purified by silica gel column chromatography (40% EtOAc in .n-Hexane) providing 51 as E/Z mixture of isomers (4.7 mg, 44%). (3f?,3aS,8a ¾-Hexahydrofuro[2,3-d]furan~3-yt ((25,3 ?)-3~! ydroxy~4-( 3- methoxy-1 ,1-dsoxido~4 _i 5,6 ₎ 7,8,9-hexa ydrQ-11 H~

benzo[cl[1]oxa[5lthia[6lazacycSotrideciri~2(3H ~yS)-1 -pheny!butari~2~ y!)carbamate (5).

[0064] Compound 51 (5 mg, 0,0079 mmoi) was dissolved in EiOAc (3 mL) and Pd/C (5 mg) was added. The reaction mixture was stirred under hydrogen atmosphere for 12 h and then filtered on a cellte plug. The reaction crude was purified by silica gel column chromatography (EtOAc) providing inhibitor 5 (4 mg, 80%).

[0065] H N R (400 MHz, CDCI ₃ ) δ 7.85 (d, J = 8.8 Hz, 1 H), 7,47 - 7, 12 (m, 5H), 7.06 (d, J = 2.6 Hz, 1 H), 6.91 (dd, J = 8.8, 2.7 Hz, 1 H), 5.65 (d, J = 5.2 Hz, 1 H), 5.04 (dd, J = 14.2, 6.3 Hz, 1 H), 4.95 (d, J = 8.6 Hz, 1 H), 4.92 - 4.82 (m, 1 H), 4.81 - 4.65 (m, 1 H), 4.03 - 3.77 (m, 8H), 3.76 - 3.58 (m, 4H), 3.33 - 3.16 (m, 3H), 3.14 - 2.97 (m, 2H), 2.96 - 2.84 (m, 1 H), 2.81 (dd, J = 14.0, 9.2 Hz, 1 H), 1 .72 - 1 .46 (m, 6H), 1 .45 - 1 .33 (m, 2H), 1 .28 (s, 4H). LRMS (ESI) m/z [M+H] ⁺ 633.3, [M+Na] ⁺ 655.2.

2-{(2R,3S)-3-Amino-2- ydroxy-4-pheriyfcutyS)-13-!iitro-2 _! 3,4,5 _! 6,9- hexahydro-11 i erao[c][1 ]oxa[5]t ia [6]aMcyc!otridec!!ie 1 , -diox de (49)

[0066] Compound 47 (16 mg, 0.026 mmo!) was dissolved in dry CH ₂ CI ₂ (16 mL) and Grubbs ! catalyst (5 mg, 0.0060 mmoi, 20 mo! %) was added to the solution. The reaction mixture was stirred at 40 °C for 3 h and then quenched by adding ethyl vinyl ether and concentrated. The crude product was purified by silica gel column chromatography (40% EtOAc in n-Hexane) providing tert-butyi ((2S,3R)-3-hydroxy-4-(13-nitro-1 ₎ 1 -dioxido-4,5,6 ₎ 9-tetrahydro-1 1 H- benzo[c][1 ]oxa[5]thia [6]azacyciotridecin-2(3H)-yi)-1 -pheny!butan-2- yl icarbamate as E/Z mixture (14 mg, 92%). This latter (10 mg, 0.0169 mmoi) was dissolved in CH ₂ CI ₂ (3 mL) and the solution was cooled to 0 °C, TFA (100 ML) was added and the reaction mixture was stirred at 25 ^C C for 6 h. Solvents were evaporated and the residue was taken up with CH ₂ Ci ₂ (12 mL), treated with 2 mL of saturated aqueous NaHC03, dried on anhydrous Na ₂ S04, filtered, and concentrated under reduced pressure. The resulting 2-((2R,3S)-3-amino-2- hydroxy-4-phenylbutyl)-13-nitro-2,3,4,5,6,9-hexahydro-1 1 H- benzo[c][1 ]oxa[5]thia [6jazacyciotrideclne 1 ,1 -dioxide (49) was used to the next step without further purification . (3f?,3aS,8a ¾-Hexahydrofuro[2,3-d]furan~3-yt ((25,3 ?)-3~! ydroxy~4-(13- rsitro-1 ,1-diioxiido-4,5,6,9-tetrahydro-11 H~

benzo[cl[1]oxa[5lthia[6lazacycSotrideciri~2(3H ~yS)-1 -pheray!butari~2~ yf)carbamate (52).

[0067] Amine 49 (8 mg, 0.0163 mmol) was dissolved in MeCN (4 mL) and N,N- DIPEA (20 μΐ.., 0.161 mmol) was added followed by know activated bis-T F derivative 50 (5 mg, 0.0169 mmol). The reaction mixture was stirred at 25 °C for 5 days, then voiatiles were evaporated under reduced pressure and the residue was purified by silica gel column chromatography (20-50% EtOAc In n-Hexane) providing 52 as E/Z mixture of isomers (5 mg, 49%).

(3/?,3aS,6a^-Hexahydrofuro[2,3-£?lfurari-3-yS ((2S,3/?)-4-(13-amino-1 ,1 - dio ido-^S^JAg-hexah dro-l 1 H~

berszo[c][1loxa[5lthia[6]azaGycSotridecsri-2(3H5-yS)-3-hy droxy-1- phersylbutan-2-yl)carbamate (7).

[0068] Compound 52 (8 mg, 0.0124 mmol) was dissolved in EtOAc (3 mL) and Pd/C (5 mg) was added. The reaction mixture was stirred under hydrogen atmosphere for 9 h and then filtered on a celite plug. The reaction crude was purified by silica gel column chromatography (EtOAc) providing Inhibitor 7 (7 mg, 91 %).

[0069] Ή NMR (400 MHz, CDCI ₃ ) δ 7.69 (d, J = 8.6 Hz, 1 H), 7.32 - 7.11 (m, 6H), 6.75 (d, J - 2.3 Hz, 1 H), 6.62 (dd, J = 8.6, 2.4 Hz, 1 H), 5.65 (d, J = 5.2 Hz, 1 H), 5.03 (dd, J = 14.2, 6.3 Hz, 1 H), 4.95 (d, J = 8.7 Hz, 1 H), 4.90 - 4.77 (m, 1 H), 4.75 - 4.63 (m, 1 H), 4.15 (s, 2H), 4.06 - 3.78 (m, 4H), 3.70 (dt, J = 10.7, 5.7 Hz, 4H), 3.49 (d, J - 4.4 Hz, 1 H), 3.30 - 3.16 (m, 2H), 3.14 - 2.98 (m, 2H), 2.97 - 2.87 (m, 1 H), 2.80 (dd, J - 13.9, 9.2 Hz, 1 H), 1 .75 - 1 .15 (m, 12H). LRMS (ES!) m/z [M+H] ⁺ 618,3, [M+Na] ⁺ 640.3.

Scheme 4. Reagents and conditions: (a) DiPEA, MeCN, 23 °C, 2 days; (b) H ₂ , 1 0% Pd/C, EtOAc, 23 °G, 12 h,

(3S,7aS,BS}- ex^ ydw- H~3,5~met Bnoium[2,3-bjpYran-8~yl ((2S,3ffi}- ~ (I S-amirio-l jl-dioxido-^^.ej^^-hexahyd o-l 1 W- beiizo[c][1]oxa[5]thia[6]azacyctotr!decin~2(3W5-y -3-hydrQxy-1~

phersyfbutar!-2~y1)earbamate (14).

[0070] Amine 49 (5.8 mg, 0.01 19 mmo!) was dissolved in MeCN (3 mL) and Ν,Ν-ΟίΡΕΑ (16 μΙ_, 0.1 19 mmol) was added followed by know activated crown- THF derivative 53 (4.2 mg, 0.0131 mmol). The reaction mixture was stirred at 25 °C for 7 days, then volatiies were evaporated under reduced pressure and the residue was purified by silica gel column chromatography (20-50% EtOAc in n -Hexane) providing (3S,7aS,8S}-Hexahydro-4H-3,5-methanofuro[2,3-b]pyran - 8-y! ((2S _! 3R)-3-hydroxy-4-(13-nitro- 1 ,1 -dioxido-4,5,6,9-tetrahydro-1 1 H- benzo[c][13oxa[5]thia[6] azacyclotridecin-2(3H)-yl)-1 -phenylbutan-2- y!}carbamate as E/Z mixture of Isomers (3.5 mg, 44%). This latter (3.5 mg, 0.0052 mmol) was dissolved in EtOAc (3 mL) and Pd/C (7 mg) was added. The reaction mixture was stirred under hydrogen atmosphere for 10 h and then filtered on a celite plug. The reaction crude was purified by silica gei column chromatography (30-70% EtOAc in n-Hexane) providing 14 (1 .6 mg, 48%). Ή NMR (800 MHz, CDC ) δ 7.70 (d, J = 8.0 Hz, 1 H), 7.33 - 7.13 (m, 6H), 6.76 (s, 1 H), 6.63 (d, J = 8.0 Hz, 1 H), 5.42 (d, J = 6.4 Hz, 1 H), 5.1 1 (s, 1 H), 5.00 (d, J = 8.0 Hz, 1 H), 4.89-4.81 (m, 2H), 4,73 - 4,61 (m, 1 H), 4.16 (s, 1 H), 3.93 - 3.84 (m, 3H), 3.77 (d, J = 9.6 Hz, 1 H), 3.69 - 3.64 (m, 2 H), 3.63 (t, J = 8.8 Hz, H), 3.57 (t, J = 7.6 Hz, 1 H), 3.28 - 3,15 (m, 3H), 3.13 - 3.10 (m, 1 H), 3.09 - 3.02 (m, 1 H), 2.88 - 2.85 (m, 1 H), 2.73 - 2.69 (m, 1 H), 2.68 - 2.64 (m, 1 H), 2.37 - 2.34 (m, 1 H), 1 ,82 (d, J = 11 .2 Hz, 1 H), 1 .69 - 1 .1 5 (m, 1 1 H). LRMS (ESI) m/z +H] ⁺ 644.2, [M+Na] ⁺ 666.3.

[0071] The disclosure provides for the following embodiments, the numbering of which is not to be construed as designating levels of importance:

[0072] Embodime ) :

(!)

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

the dashed line represents an E- or Z- double bond;

Z is:

R ¹ and R ² are each independently hydrogen, alky! or ary!alky! ;

R ³ and R ⁴ are each Independently H, alky! or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cycloaikyl or a heterocyciyi group;

Y ¹ is optionally substituted alkylene, O, or N-R ^a , wherein R ^a Is hydrogen or aikyi ; X ¹ and X ² are each independently oxygen, S, S(O), S0 ₂ , optionally substituted nitrogen, or optionally substituted alkylene;

each R ⁵ Is independently hydrogen, -OR", wherein R ^b is alky! or aryl , -SO2R ⁷ , - NR ⁷ 2, -CHR ⁷ OR ⁷ or CR ⁷ ₃ , wherein each R ⁷ is independently hydrogen, aikyl, heteroaiky! or heterocyclyl or two adjacent R ⁵ groups, together with the carbon atoms to which they are attached, form a heferoaryi group;

each R ⁶ is independently hydrogen, halo, -NR ⁷ 2, aikyl, aryl, aikylaryl , heteroaiky! or heterocyclyl;

y is an integer from 1 to 3;

p is an integer from 1 to 3; and

n is an integer from 0-4,

[0073] Embodiment 2 relates to the compound of Embodiment 1 , wherein each R ⁵ is independently hydrogen, -OR ^b , wherein R ^b is aikyl or aryl or -NR ⁷ ₂ , wherein each R ⁷ is independently hydrogen, alkyi, heteroaiky! or heterocyclyl .

[0074] Embodiment 3 relates to the compound of Embodiment 1 ,

R ¹ and R ² are each independently hydrogen, aikyl or arylalky! ; R ³ and R ⁴ are each Independently H, alky! or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalkyl or a heterocyc!yl group;

Y ¹ is optionally substituted aikylene, O, or N-R ^a , wherein R ^a is hydrogen or aikyi ; X ¹ and X ² are each independently oxygen, S, S(O), SOz, optionally substituted nitrogen, or optiona!!y substituted aikylene;

each R ⁶ is Independently hydrogen, halo, -NR ⁷ 2, aikyi, aryl, aikyiaryl , heteroalkyl or heterocyciyi ;

R ¹⁰ is R ⁹ or -NR ⁹ 2, wherein each R ⁹ is independently hydrogen, aikyi, cycloaikyl, heteroalkyl or heterocyciyi ;

Y ² and Y ³ are each independently NH, S, O, NR ^S or CR ⁿ ₂ , wherein each R ^{1 1} is independently hydrogen, alky! , aryl, or heterocyciyi ;

p is an integer from 1 to 3; and

n is an integer from 0-4.

[0075] Embodiment 4 relates to the compound of Embodiments 1 -3,

[0078] Embodiment 5 relates to the compound of Embodiments 1 -4, wherein Y ¹ is optionally substituted aikylene,

[0077] Embodiment 6 relates to the compound of Embodiments 1 -5, wherein Y ¹ is -(CH2)m-Q-, wherein m Is an Integer from 1 -3 and Q is O or N-R ^a , wherein R ^a is hydrogen or aikyi.

[0078] Embodiment 7 relates to the compound of Embodiments 1 -6, wherein Y ¹ is -CH ₂ -.

[0079] Embodiment 8 relates to the compound of Embodiment 1 , wherein the compound of the formula (I) is a compound of the formula (lb):

(lb)

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

the dashed line represents an E- or Z- double bond;

R ¹ and R ² are each independently hydrogen, alkyl or arylalkyl ;

R ³ and R ⁴ are each Independently H, alkyl or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalkyl or a heterocyc!yl group;

X ^! and X ² are each independently oxygen, S, S(O), S0 ₂ , optionally substituted nitrogen, or optionally substituted a!ky!ene;

R ⁵ Is hydrogen, -GR ^b , wherein R ^b is alkyl or aryi, -SQ ₂ R ⁷ , -NR ⁷ ₂ , -CHROR ⁷ or CR ⁷ 3, wherein each R ⁷ is independently hydrogen, alkyl, heteroalkyl or heterocyclyl or two adjacent R ⁵ groups, together with the carbon atoms to which they are attached, form a heteroaryl group;

each R ⁶ is Independently hydrogen, halo, -NR ⁷ ₂ , aikyi, aryl, aikyiaryl , heteroalkyl or heterocyclyl;

p is an integer from 1 to 3; and

n is an integer from 0-4.

[0080] Embodiment 9 relates to a compound of the formula (II):

or a pharmaceutically acceptable salt, isomer, mixture of isomers, crystalline form, non crystalline form, hydrate, or solvate thereof wherein:

wherein each R ⁹ is Independently hydrogen, a!kyl, heieroalky! or heterocyc!yl ; R ¹ and R ² are each independently hydrogen, alky! or ary!alkyl ;

R ³ and R ⁴ are each Independently H, alky! or R ³ and R ⁴ , together with the carbon atom to which they are attached, form a cyc!oalkyl or a heterocyc!yl group;

X ¹ and X ² are each independently oxygen, S, S(O), S0 ₂ , optionally substituted nitrogen, or optionally substituted a!ky!ene;

each R ⁵ is independently hydrogen, -OR ^b , wherein R ^b is aikyl or ary! , -S0 ₂ R ⁷ , - NR ⁷ 2, -CHR' R ⁷ or CR ⁷ 3, wherein each R ⁷ is independently hydrogen, aikyl, heteroa!kyl or heterocyc!yl or two adjacent R ⁵ groups, together with the carbon atoms to which they are attached, form a heteroaryl group;each R ⁶ is independently hydrogen, halo, -NR ⁷ ₂ , a!ky!, ary!, aikyiaryi, heieroalky! or heterocyc!y! ;

y is an integer from 1 to 3;

p is an integer from 1 to 3; and

n is an integer from 0-4.

[0081] Embodiment 10 relates to the compound of Embodiment 9, wherein each R ⁵ is independently hydrogen , -OR ^b , wherein R ^b is alky! or ary! , or -NR ⁷ 2, wherein each R ⁷ is independently hydrogen, a!ky!, heteroa!kyi or heterocyclyi ,

[0082] Embodiment 11 relates to the compound of Embodiment 9, wherein each R ⁵ is Independently hydrogen , -QR ^b , wherein R ^b Is aikyl, or -NR ⁷ ₂ , wherein each R ⁷ is independently hydrogen, aikyl, heteroalkyl or heterocyclyi.

[0083] Embodiment 12 relates to the compound of Emnbodiments 1 -1 1 , wherein R ¹ and R ² are each hydrogen,

[0084] Embodiment 13 relates to the compound of Embodiments 1 -12, wherein X ¹ and X ² are each independently oxygen, S, S0 ₂ or optionally substituted alkylene.

[0085] Embodiment 14 relates to the compound of Embodiments 1 -13, wherein R ³ and R ⁴ are each hydrogen.

[0086] Embodiment 15 relates to the compound of Embodiments 1 -14, wherein y is 1 .

[0087] Embodiment 16 relates to the compound of Embodiments 1 -15, wherein p is 1 or 2.

[0088] Embodiment 17 relates to the compound of Embodiments 1 -16, wherein n is an integer from 1 -4.

[0089] Embodiment 18 relates to a compound of the formula:

6335

PCT/US2018/040342





[QQ90] Embodiment 1 9 relates to a pharmaceutical composition comprising one or more compounds of any one of Embodiments 1 -18 and one or more pharmaceutically acceptable carriers, diluents, exclplents or combinations thereof.

[0091] Embodiment 20 relates to a method for treating a patient in need of relief from HIV/AIDS, the method comprising the step of administering to the patient a therapeutically effective amount of a compound of any one of Embodiments 1 -1 8 or a pharmaceutical composition of Embodiment 19.