Title:
MACROPHAGE SPECIFIC CHIMERIC ANTIGEN RECEPTOR, CONTROLLABLE POLARIZED MONOCYTE/MACROPHAGE EXPRESSING RECEPTOR, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/262130
Kind Code:
A1
Abstract:
The present disclosure relates to the field of biotechnology, and provides a macrophage specific chimeric antigen receptor, a controllable polarized monocyte/macrophage expressing the receptor, a preparation method therefor and an application thereof. The present disclosure provides a chimeric antigen receptor, comprising an extracellular antigen-binding domain, a transmembrane domain, and an intracellular activation domain connected in sequence. The extracellular antigen-binding domain comprises a signal peptide and/or scFv, and can specifically recognize a cell membrane surface protein EGFRvIII specifically expressed by GBM. The transmembrane domain comprises CD8α, and is linked to the extracellular antigen-binding domain and the intracellular activation domain. The intracellular activation domain comprises TIR, CD3ZETA, or GM-CSFRα/βCD3, and promotes the polarization of macrophage into M1 type. The chimeric antigen receptor of the present disclosure is introduced into macrophage, so that the macrophage has a targeted killing effect on GBM, and the M1 polarization state of the macrophage is effectively promoted and maintained.
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Inventors:
ZHANG JIN (CN)
LEI ANHUA (CN)
TIAN LIN (CN)
LEI ANHUA (CN)
TIAN LIN (CN)
Application Number:
PCT/CN2021/117808
Publication Date:
December 22, 2022
Filing Date:
September 10, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C07K19/00; A61K39/00; A61P35/00; C12N5/10; C12N15/867
Foreign References:
| CN106636003A | 2017-05-10 | |||
| CN106967685A | 2017-07-21 | |||
| CN111139256A | 2020-05-12 |
Attorney, Agent or Firm:
CHOFN INTELLECTUAL PROPERTY (CN)
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