Title:
MAGNETIC LYSIS METHOD AND DEVICE
Document Type and Number:
WIPO Patent Application WO/2011/034621
Kind Code:
A3
Abstract:
A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
Inventors:
BELGRADER PHILIP (US)
HINDSON BENJAMIN (US)
HINDSON BENJAMIN (US)
Application Number:
PCT/US2010/002569
Publication Date:
November 24, 2011
Filing Date:
September 20, 2010
Export Citation:
Assignee:
AKONNI BIOSYSTEMS (US)
QUANTALIFE INC (US)
BELGRADER PHILIP (US)
HINDSON BENJAMIN (US)
QUANTALIFE INC (US)
BELGRADER PHILIP (US)
HINDSON BENJAMIN (US)
International Classes:
B01F13/08; B01F9/10; B02C17/20; C12M1/33; C12N13/00
Foreign References:
| EP1234832A2 | 2002-08-28 | |||
| US20030203491A1 | 2003-10-30 | |||
| US20080063628A1 | 2008-03-13 | |||
| US5536475A | 1996-07-16 | |||
| US20080102479A1 | 2008-05-01 | |||
| US20040082779A1 | 2004-04-29 |
Other References:
CLARISSA LUI ET AL.: "Nucleic Acid-based Detection of Bacterial Pathogens Usi ng Integrated Microfluidic Platform Systems", SENSORS, 2009, vol. 9, 18 May 2009 (2009-05-18), pages 3713 - 3744, XP008153548
JAMES E. SMOLEN ET AL.: "L-Selectin Signaling of Neutrophil Adhesion and Degr anulation Involves p38 Mitogen-activated Protein Kinase", THE JOURNAL OF BIOLO GICAL CHEMISTRY, vol. 275, no. 21, 2000, pages 15876 - 15884, XP008153492
ROBERT J. TROTMAN ET AL.: "Calcium Alginate Bead Immobilization of Cells Cont aining Tyrosine Ammonia Lyase Activity for Use in the Production of p-Hydrox ycinnamic Acid", BIOTECHNOL. PROG., vol. 23, 2007, pages 638 - 644, XP002461391
PETRA S. DITTRICH ET AL.: "Micro Total Analysis Systems. Latest Advancements and Trends", ANAL. CHEM., vol. 78, 2006, pages 3887 - 3907, XP008153490
See also references of EP 2480324A2
JAMES E. SMOLEN ET AL.: "L-Selectin Signaling of Neutrophil Adhesion and Degr anulation Involves p38 Mitogen-activated Protein Kinase", THE JOURNAL OF BIOLO GICAL CHEMISTRY, vol. 275, no. 21, 2000, pages 15876 - 15884, XP008153492
ROBERT J. TROTMAN ET AL.: "Calcium Alginate Bead Immobilization of Cells Cont aining Tyrosine Ammonia Lyase Activity for Use in the Production of p-Hydrox ycinnamic Acid", BIOTECHNOL. PROG., vol. 23, 2007, pages 638 - 644, XP002461391
PETRA S. DITTRICH ET AL.: "Micro Total Analysis Systems. Latest Advancements and Trends", ANAL. CHEM., vol. 78, 2006, pages 3887 - 3907, XP008153490
See also references of EP 2480324A2
Attorney, Agent or Firm:
YE, Michael et al. (1350 I Street N.w.,Suite 110, Washington DC, US)
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