Title:
MAGNETIC NANOPARTICLE-SAMIRNA COMPLEX AND METHOD FOR PREPARING SAME
Document Type and Number:
WIPO Patent Application WO/2013/109057
Kind Code:
A1
Abstract:
The present invention relates to a SAMiRNA-magnetic nanoparticle complex capable of efficiently delivering double-stranded RNA oligonucleotides and magnetic nanoparticle to cells and to a composition comprising the SAMiRNA-magnetic nanoparticle complex, capable of both diagnosing and treating diseases such as cancer. More particularly, the present invention relates to a SAMiRNA-magnetic nanoparticle complex wherein a double-stranded RNA oligonucleotide-polymer structure in which double-stranded RNA oligonucleotides and a hydrophilic material and a second hydrophobic material are connected by a simple covalent bond or a linker mediated covalent bond and a magnetic nanoparticle in which a first hydrophobic material is bonded to the surface of a magnetic material are formed into a core. The first hydrophobic material of the present invention performs a hydrophobic interaction with the second hydrophobic material of the double-stranded RNA oligonucleotide structure so as to form SAMiRNA-magnetic nanoparticle complexes having a uniform size. In addition, the hydrophilic material and the second hydrophobic material bonded in the double-stranded RNA oligonucleotide structure may improve in vivo stability of the double-stranded RNA oligonucleotides. A ligand which is additionally bonded to the structure may deliver a SAMiRNA-magnetic nanoparticle complex to a target cell even when a small amount of the ligand is applied, and a magnetic material of the magnetic nanoparticle may be used as an imaging agent for diagnosis.
Inventors:
CHAE JEIWOOK (KR)
CHUNG HYUNJIN (KR)
LEE BOREUM (KR)
PARK HAN OH (KR)
CHUNG HYUNJIN (KR)
LEE BOREUM (KR)
PARK HAN OH (KR)
Application Number:
PCT/KR2013/000360
Publication Date:
July 25, 2013
Filing Date:
January 17, 2013
Export Citation:
Assignee:
BIONEER CORP (KR)
International Classes:
A61K9/16; A61K31/7088; A61K31/7105; A61K33/24; A61K33/243; A61K33/244; A61K47/48
Foreign References:
| KR100819378B1 | 2008-04-04 | |||
| KR100883471B1 | 2009-02-16 | |||
| KR20090042297A | 2009-04-29 | |||
| KR20070102672A | 2007-10-19 |
Other References:
LEE, J. H. ET AL.: "All-in-one target-cell-specific magnetic nanoparticles for simultaneous molecular imaging and siRNA delivery", ANGEW. CHEM., vol. 121, 2009, pages 4238 - 4243, XP055035302
VEISEH, O. ET AL.: "Chlorotoxin bound magnetic nanovector tailored for cancer cell targeting, imaging, and siRNA delivery", BIOMATERIALS, vol. 31, 2010, pages 8032 - 8042, XP055014008
KENNY, G. D. ET AL.: "Novel multifunctional nanoparticle mediates siRNA tumour delivery, visualisation and therapeutic tumour reduction in vivo", JOURNAL OF CONTROLLED RELEASE, vol. 149, 2011, pages 111 - 116, XP027595345
BAE, K. H. ET AL.: "Surface functionalized hollow manganese oxide nanoparticles for cancer targeted siRNA delivery and magnetic resonance imaging", BIOMATERIALS, vol. 32, 2011, pages 176 - 184, XP027493702
VEISEH, O. ET AL.: "Cell transcytosing poly-arginine coated magnetic nanovector for safe and effective siRNA delivery", BIOMATERIALS, vol. 32, 2011, pages 5717 - 5725, XP028375332
ACC. CHEM. RES., vol. 44, no. 10, 2011, pages 1018 - 1028
TAEEOK KIM: "nano-biotechnology", 2009, BIOTECH POLICY RESEARCH CENTER
TOSHIHIRO MATSUO ET AL., J. BIOMEDICAL MATERIALS RESEARCH PART A, vol. 66A, no. 4, 2003, pages 747 - 754
XIAOLIZHENG ET AL., INTERNATIONAL J.PHARMACEUTICS, vol. 366, 2009, pages 211 - 217
POLYMER SCIENCE AND TECHNOLOGY, vol. 19, no. 2, 2008, pages 116 - 124
DANA J. GARY ET AL., JOURNAL OF CONTROLLED RELEASE, vol. 121, 2007, pages 64 - 73
"Silencing of microRNAs in vivo with 'antagomirs", NATURE, vol. 438, no. 7068, December 2005 (2005-12-01), pages 685 - 689
"MicroRNAs as Therapeutic Targets", NEW ENGLAND J. MEDICINE, vol. 354, no. 11, 2006, pages 1194 - 1195
MEISTER G. ET AL.: "Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing", RNA, vol. 10, no. 3, March 2004 (2004-03-01), pages 544 - 550, XP002996109, DOI: doi:10.1261/rna.5235104
"Survivin: a new target for anti-cancer therapy", CANCER TREAT REV., vol. 35, no. 7, November 2009 (2009-11-01), pages 553 - 62
KENNETH A. DAWSON ET AL., NATURE NANOTECHNOLOGY, vol. 4, 2009, pages 84 - 85
See also references of EP 2805713A4
VEISEH, O. ET AL.: "Chlorotoxin bound magnetic nanovector tailored for cancer cell targeting, imaging, and siRNA delivery", BIOMATERIALS, vol. 31, 2010, pages 8032 - 8042, XP055014008
KENNY, G. D. ET AL.: "Novel multifunctional nanoparticle mediates siRNA tumour delivery, visualisation and therapeutic tumour reduction in vivo", JOURNAL OF CONTROLLED RELEASE, vol. 149, 2011, pages 111 - 116, XP027595345
BAE, K. H. ET AL.: "Surface functionalized hollow manganese oxide nanoparticles for cancer targeted siRNA delivery and magnetic resonance imaging", BIOMATERIALS, vol. 32, 2011, pages 176 - 184, XP027493702
VEISEH, O. ET AL.: "Cell transcytosing poly-arginine coated magnetic nanovector for safe and effective siRNA delivery", BIOMATERIALS, vol. 32, 2011, pages 5717 - 5725, XP028375332
ACC. CHEM. RES., vol. 44, no. 10, 2011, pages 1018 - 1028
TAEEOK KIM: "nano-biotechnology", 2009, BIOTECH POLICY RESEARCH CENTER
TOSHIHIRO MATSUO ET AL., J. BIOMEDICAL MATERIALS RESEARCH PART A, vol. 66A, no. 4, 2003, pages 747 - 754
XIAOLIZHENG ET AL., INTERNATIONAL J.PHARMACEUTICS, vol. 366, 2009, pages 211 - 217
POLYMER SCIENCE AND TECHNOLOGY, vol. 19, no. 2, 2008, pages 116 - 124
DANA J. GARY ET AL., JOURNAL OF CONTROLLED RELEASE, vol. 121, 2007, pages 64 - 73
"Silencing of microRNAs in vivo with 'antagomirs", NATURE, vol. 438, no. 7068, December 2005 (2005-12-01), pages 685 - 689
"MicroRNAs as Therapeutic Targets", NEW ENGLAND J. MEDICINE, vol. 354, no. 11, 2006, pages 1194 - 1195
MEISTER G. ET AL.: "Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing", RNA, vol. 10, no. 3, March 2004 (2004-03-01), pages 544 - 550, XP002996109, DOI: doi:10.1261/rna.5235104
"Survivin: a new target for anti-cancer therapy", CANCER TREAT REV., vol. 35, no. 7, November 2009 (2009-11-01), pages 553 - 62
KENNETH A. DAWSON ET AL., NATURE NANOTECHNOLOGY, vol. 4, 2009, pages 84 - 85
See also references of EP 2805713A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
이처영 (KR)
이처영 (KR)
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