Title:
MAMMALIAN GENE MODIFICATION METHOD USING ELECTROPORATION
Document Type and Number:
WIPO Patent Application WO/2015/049897
Kind Code:
A1
Abstract:
The present invention addresses the problem of developing a technology that can be widely used in mammals but does not require the use of ES cells, wherein the technology (genome editing technology such as ZFN) is for modifying a specific gene using a specific sequence on a genome as the target, and the technology can be used with nothing more than a very simple method. Provided is a technology for modifying with good efficiency any target mammalian gene by immersing a fertilized mammalian egg that has a clear zone and is in the pronuclear stage in a solution containing pair molecules of mRNA having a specific sequence, and performing electroporation by applying three-step square wave multipulses, with the total amount of electricity of the first electrical pulse set to be within a predetermined range.
Inventors:
KANEKO TAKEHITO (JP)
MASHIMO TOMOJI (JP)
HAYAKAWA YASUHIKO (JP)
HAYAKAWA KIYOSHI (JP)
MASHIMO TOMOJI (JP)
HAYAKAWA YASUHIKO (JP)
HAYAKAWA KIYOSHI (JP)
Application Number:
PCT/JP2014/063910
Publication Date:
April 09, 2015
Filing Date:
May 27, 2014
Export Citation:
Assignee:
NEPA GENE CO LTD (JP)
International Classes:
C12N15/877; A01K67/027
Domestic Patent References:
| WO2011158845A1 | 2011-12-22 |
Foreign References:
| JP2011147399A | 2011-08-04 | |||
| JP2002238651A | 2002-08-27 |
Other References:
TOMOJI MASHIMO ET AL.: "Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes", SCIENTIFIC REPORTS, vol. 3, 13 February 2013 (2013-02-13), pages 1 - 6, XP055331633, DOI: 10.1038/SREP01253
SUSUMU KONO ET AL.: "A study of material incorporation for medaka (Oryzias latipes) EGGS by various voltage pulses", PROC. OF THE 18TH IEEE INTERNATIONAL PULSED POWER CONFERENCE (PPC, June 2011 (2011-06-01), pages 1165 - 1170, XP032172963
TAKAHIRO KUBO ET AL.: "Investigation of Experimental Conditions for Incorporation of Chemical Substance into Fertilized Medaka Eggs by Applying Voltage Pulses", THE INSTITUTE OF ELECTRICAL ENGINEERS OF JAPAN KENKYUKAI SHIRYO, 8 March 2012 (2012-03-08), pages 5 - 8, XP008183230
ARON M. GEURTS ET AL.: "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", SCIENCE, vol. 325, no. 5939, 2009, pages 433, XP002599999
JOANNA B. GRABAREK ET AL.: "Efficient delivery of dsRNA into zona-enclosed mouse oocytes and preimplantation embryos by electroporation", GENESIS, vol. 32, no. 4, 2002, pages 269 - 276, XP055331639
SEUNG WOO CHO ET AL.: "Targeted genome engineering in human cells with the Cas9 RNA- guided endonuclease", NATURE BIOTECHNOLOGY, vol. 31, 29 January 2013 (2013-01-29), pages 230 - 232, XP002699850
HUI PENG ET AL.: "Efficient Delivery of DNA and Morpholinos into Mouse Preimplantation Embryos by Electroporation", PLOS ONE, vol. 7, no. 8, 21 August 2012 (2012-08-21), pages E43748, XP055331643
"KAGAKU TO SEIBUTSU", 2011, article "Zinc Finger Nuclease (ZFN", pages: 220 - 222
TOMOJI MASHIMO; TADAO SERIKAWA, ZINC FINGER NUCLEASE (ZFN): CELL TECHNOLOGY, vol. 31, no. 3, 2012, pages 296 - 301
TAKASHI YAMAMOTO; SUMIHARE NOJI: "Genome Editing Revolution", CELL TECHNOLOGY V, vol. 32, no. 5, 2013
RYAN M. WALSH; KONRAD HOCHEDLINGER, PNAS, vol. 110, no. 39, 2013, pages 15514 - 15515
JOANNA B. GRABAREK ET AL., GENESIS, vol. 32, 2002, pages 269 - 276
HUI PENG ET AL., PLOS ONE, vol. 7, no. 8, 2012, pages E43748,1 - 13
SHIMOGAWARA, K. ET AL., GENETICS, vol. 148, 1998, pages 1821 - 1828
SUKHAREV S.I. ET AL., BIOPHYS. J., vol. 63, 1992, pages 1320 - 1327
See also references of EP 3054015A4
SUSUMU KONO ET AL.: "A study of material incorporation for medaka (Oryzias latipes) EGGS by various voltage pulses", PROC. OF THE 18TH IEEE INTERNATIONAL PULSED POWER CONFERENCE (PPC, June 2011 (2011-06-01), pages 1165 - 1170, XP032172963
TAKAHIRO KUBO ET AL.: "Investigation of Experimental Conditions for Incorporation of Chemical Substance into Fertilized Medaka Eggs by Applying Voltage Pulses", THE INSTITUTE OF ELECTRICAL ENGINEERS OF JAPAN KENKYUKAI SHIRYO, 8 March 2012 (2012-03-08), pages 5 - 8, XP008183230
ARON M. GEURTS ET AL.: "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", SCIENCE, vol. 325, no. 5939, 2009, pages 433, XP002599999
JOANNA B. GRABAREK ET AL.: "Efficient delivery of dsRNA into zona-enclosed mouse oocytes and preimplantation embryos by electroporation", GENESIS, vol. 32, no. 4, 2002, pages 269 - 276, XP055331639
SEUNG WOO CHO ET AL.: "Targeted genome engineering in human cells with the Cas9 RNA- guided endonuclease", NATURE BIOTECHNOLOGY, vol. 31, 29 January 2013 (2013-01-29), pages 230 - 232, XP002699850
HUI PENG ET AL.: "Efficient Delivery of DNA and Morpholinos into Mouse Preimplantation Embryos by Electroporation", PLOS ONE, vol. 7, no. 8, 21 August 2012 (2012-08-21), pages E43748, XP055331643
"KAGAKU TO SEIBUTSU", 2011, article "Zinc Finger Nuclease (ZFN", pages: 220 - 222
TOMOJI MASHIMO; TADAO SERIKAWA, ZINC FINGER NUCLEASE (ZFN): CELL TECHNOLOGY, vol. 31, no. 3, 2012, pages 296 - 301
TAKASHI YAMAMOTO; SUMIHARE NOJI: "Genome Editing Revolution", CELL TECHNOLOGY V, vol. 32, no. 5, 2013
RYAN M. WALSH; KONRAD HOCHEDLINGER, PNAS, vol. 110, no. 39, 2013, pages 15514 - 15515
JOANNA B. GRABAREK ET AL., GENESIS, vol. 32, 2002, pages 269 - 276
HUI PENG ET AL., PLOS ONE, vol. 7, no. 8, 2012, pages E43748,1 - 13
SHIMOGAWARA, K. ET AL., GENETICS, vol. 148, 1998, pages 1821 - 1828
SUKHAREV S.I. ET AL., BIOPHYS. J., vol. 63, 1992, pages 1320 - 1327
See also references of EP 3054015A4
Attorney, Agent or Firm:
YANO HIROYA (JP)
Hironari Yano (JP)
Hironari Yano (JP)
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