Title:
MAMMALIAN SPERM PREPARATION METHOD, ARTIFICIAL INSEMINATION METHOD, AND IN-VITRO FERTILIZATION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/145631
Kind Code:
A1
Abstract:
This mammalian sperm preparation method includes: culturing of mammalian sperm in a medium that contains TLR7 ligand and that has a pH of 7.7-7.9; inducing the acrosomal reaction of Y chromosome-containing sperm; and obtaining a sperm group that is rich with X chromosome-containing sperm and that retains acrosome. This sperm group is used for artificial insemination and in-vitro fertilization for selective male production.
Inventors:
SHIMADA MASAYUKI (JP)
UMEHARA TAKASHI (JP)
UMEHARA TAKASHI (JP)
Application Number:
PCT/JP2023/001637
Publication Date:
August 03, 2023
Filing Date:
January 20, 2023
Export Citation:
Assignee:
UNIV HIROSHIMA (JP)
International Classes:
C12N5/076; A61K31/437; A61K31/522; A61P15/08
Domestic Patent References:
| WO2019004217A1 | 2019-01-03 |
Other References:
UMEHARA TAKASHI; TSUJITA NATSUMI; ZHU ZHENDONG; IKEDO MOEKA; SHIMADA MASAYUKI: "A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 15, no. 8, 17 July 2020 (2020-07-17), GB , pages 2645 - 2667, XP037207392, ISSN: 1754-2189, DOI: 10.1038/s41596-020-0348-y
FA REN;HUAMING XI;YIJIE REN;YU LI;FEI WEN;MING XIAN;MENGJIE ZHAO;DAWEI ZHU;LIQIANG WANG;ANMIN LEI;JIANHONG HU: "TLR7/8 signalling affects X-sperm motility via the GSK3 a/B-hexokinase pathway for the efficient production of sexed dairy goat embryos", JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, BIOMED CENTRAL LTD, LONDON, UK, vol. 12, no. 1, 3 August 2021 (2021-08-03), London, UK , pages 1 - 17, XP021294570, DOI: 10.1186/s40104-021-00613-y
FA REN;HUAMING XI;YIJIE REN;YU LI;FEI WEN;MING XIAN;MENGJIE ZHAO;DAWEI ZHU;LIQIANG WANG;ANMIN LEI;JIANHONG HU: "TLR7/8 signalling affects X-sperm motility via the GSK3 a/B-hexokinase pathway for the efficient production of sexed dairy goat embryos", JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, BIOMED CENTRAL LTD, LONDON, UK, vol. 12, no. 1, 3 August 2021 (2021-08-03), London, UK , pages 1 - 17, XP021294570, DOI: 10.1186/s40104-021-00613-y
Attorney, Agent or Firm:
KIMURA Mitsuru et al. (JP)
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