Title:
MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS
Document Type and Number:
WIPO Patent Application WO/2011/011463
Kind Code:
A3
Abstract:
Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
Inventors:
BAILEY SHAUN (US)
VICK BERTRAND (US)
MOSELEY JEFF (US)
VICK BERTRAND (US)
MOSELEY JEFF (US)
Application Number:
PCT/US2010/042666
Publication Date:
October 06, 2011
Filing Date:
July 20, 2010
Export Citation:
Assignee:
AURORA BIOFUELS INC (US)
BAILEY SHAUN (US)
VICK BERTRAND (US)
MOSELEY JEFF (US)
BAILEY SHAUN (US)
VICK BERTRAND (US)
MOSELEY JEFF (US)
International Classes:
C12N15/09; C07H21/00
Domestic Patent References:
| WO2008106803A1 | 2008-09-12 |
Foreign References:
| US20030049720A1 | 2003-03-13 |
Other References:
POLLOCK: "HIGH CARBON DIOXIDE REQUIRING MUTANTS OF CHLAMYDOMONAS REINHARDTII.", HIGH CARBON DIOXIDE REQUIRING MUTANTS OF CHLAMYDOMONAS REINHARDTII., December 2003 (2003-12-01), pages 26 - 27, 30, Retrieved from the Internet [retrieved on 20101014]
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DATABASE GENBANK [online] 23 June 2008 (2008-06-23), PAN, retrieved from URL:http:://www.ncbi.nlm.nih.gov/nucest/110745458 Database accession no. EE109892
DATABASE GENBANK [online] 23 June 2008 (2008-06-23), PAN, retrieved from http://www.ncbi.nlm.nih.gov/nucest/110745465 Database accession no. EE109907
DATABASE GENBANK [online] 31 October 2006 (2006-10-31), HENRIQUEZ ET AL., retrieved from http://www.ncbi.nlm.nih.gov/protein/121951692 Database accession no. Q07CY9
Attorney, Agent or Firm:
BATHURST, Brian, K. et al. (120 Constitution DriveMenlo Park, CA, US)
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