Title:
MANNANASE PMAN5A MUTANT HAVING IMPROVED HEAT RESISTANCE, GENE THEREOF, AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2019/214702
Kind Code:
A1
Abstract:
A mannanase mutant having improved heat resistance, a gene thereof, and an application. A penicillium-derived GH5 family mannanase PMan5A is used as a parent, and molecular biological means are used to mutate histidine (H), phenylalanine (F), leucine (L) and alanine (A) at sites 93, 94, 356 and 389 in the parent into tyrosine (Y), tyrosine (Y), histidine (H) and proline (P). Result show that single-point mutants H93Y, L356H and A389P all have better heat resistance than wild-type PMan5A, and the heat resistance of a combination mutant shows a synergistic effect of the single-point mutations, indicating that sites 93, 94, 356 and 389 of PMan5A play an important role in the heat resistance of GH5 family mannanase.
Inventors:
LUO HUIYING (CN)
YAO BIN (CN)
LIU WEINA (CN)
GU YUAN (CN)
TU TAO (CN)
WANG YUAN (CN)
WANG YARU (CN)
HUANG HUOQING (CN)
BAI YINGGUO (CN)
SU XIAOYUN (CN)
MENG KUN (CN)
YAO BIN (CN)
LIU WEINA (CN)
GU YUAN (CN)
TU TAO (CN)
WANG YUAN (CN)
WANG YARU (CN)
HUANG HUOQING (CN)
BAI YINGGUO (CN)
SU XIAOYUN (CN)
MENG KUN (CN)
Application Number:
PCT/CN2019/086348
Publication Date:
November 14, 2019
Filing Date:
May 10, 2019
Export Citation:
Assignee:
FEED RES INST CAAS (CN)
International Classes:
C12N9/42; C12N15/81; C12R1/84
Domestic Patent References:
| WO2014131520A1 | 2014-09-04 |
Foreign References:
| CN108559739A | 2018-09-21 | |||
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| US20110294166A1 | 2011-12-01 |
Other References:
LIU, WEINA ET AL.: "Insight into the Thermophilic Mechanism of a Glycoside Hydrolase Family 5 beta -Mannanase", J. AGRIC. FOOD CHEM., vol. 67, no. 1, 6 December 2018 (2018-12-06), pages 473 - 483, XP055650789, DOI: 10.1021/acs.jafc.8b04860
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY, pages: 6.3.1 - 6.3.6
SAMBROOK ET AL.: "Molecular cloning"
See also references of EP 3792356A4
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY, pages: 6.3.1 - 6.3.6
SAMBROOK ET AL.: "Molecular cloning"
See also references of EP 3792356A4
Attorney, Agent or Firm:
FASIN IP & PARTNERS (CN)
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