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Title:
MARKER FOR DETECTING HIGHLY PATHOGENIC INFLUENZA VIRUS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/101747
Kind Code:
A3
Abstract:
The present invention relates to a marker for detecting a highly pathogenic influenza virus and a use thereof, and more particularly, to a marker for detecting a highly pathogenic influenza virus, which includes a protein mutant prepared by substituting the amino acids 68 and 69 of a PB1-F2 protein, a composition for detecting a highly pathogenic virus, which includes an agent for measuring the protein mutant, and a detection kit including the same, a method for detecting a highly pathogenic virus, which includes measuring the protein mutant, an antiviral composition against influenza A virus, which includes an inhibitor of binding between a PB1-F2 protein in which the amino acids 68 and 69 are substituted and DDX3, and a method for screening an antiviral substance against influenza A virus. In the present invention, as the correlation between a 1918 PB1-F2 protein and high pathogenicity of the virus and a molecular mechanism thereof were first identified, a protein mutant prepared by substituting the amino acids 68 and 69 in the PB1-F2 protein sequence of an influenza virus can be used as a marker for detecting a highly pathogenic virus, and a highly pathogenic virus may be effectively detected by measuring the mutant. In addition, the viral mechanism of the evasion of innate immunity by the PB1-F2 protein mutant newly identified in the present invention may provide a new understanding for developing an antiviral agent, and the antiviral composition according to the present invention may be effectively used in the development of an antiviral agent.

Inventors:
KIM KYUN-HWAN (KR)
PARK EUN SOOK (KR)
PARK YEONG-MIN (KR)
SEONG BAIK LIN (KR)
BYUN YOUNG HO (KR)
LEE HYE MIN (KR)
Application Number:
PCT/KR2017/013843
Publication Date:
July 26, 2018
Filing Date:
November 29, 2017
Export Citation:
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Assignee:
DANDI BIOSCIENCE INC (KR)
International Classes:
G01N33/569; G01N33/50; G01N33/68
Foreign References:
US20120282593A12012-11-08
Other References:
ALYMOVA, I. V. ET AL.: "A novel cytotoxic sequence contibutes to influenza A viral protein PB1-F2 pathogenicity and predisposition to secondary bacterial infection", JOURNAL OF VIROLOGY, vol. 88, no. 1, January 2014 (2014-01-01), pages 503 - 515, XP055506711, Retrieved from the Internet
CONENELLO, G. M. ET AL.: "A single mutation in the PB1-F2 of H5N1 ( HK /97) and 1918 influenza A viruses contributes to increased virulence", PLOS PATHOGEN, vol. 3, no. 10, 5 October 2007 (2007-10-05), pages 1414 - 1421, XP055506713, Retrieved from the Internet
CONENELLO, G. M. ET AL.: "A single N66S mutation in the PB1-F2 protein of influenza A virus increases virulence by inhibiting the early interferon response in vivo", JOURNAL OF VIROLOGY, vol. 85, no. 2, January 2011 (2011-01-01), pages 652 - 662, XP055506715, Retrieved from the Internet
RAMAN, S. N. T. ET AL.: "DDX3 interacts with influenza A virus NS1 and NP proteins and exerts antiviral function through regulation of stress granule format ion", JOURNAL OF VIROLOGY, vol. 90, no. 7, April 2016 (2016-04-01), pages 3661 - 3675, XP055506716, Retrieved from the Internet
See also references of EP 3548893A4
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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