Title:
MASS SPECTROMETER INCORPORATING HYDROGEN-DEUTERIUM EXCHANGE
Document Type and Number:
WIPO Patent Application WO/2011/098833
Kind Code:
A3
Abstract:
A mass spectrometer is disclosed comprising a hydrogen-deuterium exchange cell. Isomeric ions having different conformations but substantially similar ion mobilities can be differentiated by subjecting the ions to hydrogen-deuterium exchange. Two ions having similar ion mobilities can be differentiated more effectively if they have different surface conformations by determining the relative degree of hydrogen-deuterium exchange.
Inventors:
BROWN JEFFERY MARK (GB)
Application Number:
PCT/GB2011/050273
Publication Date:
October 06, 2011
Filing Date:
February 14, 2011
Export Citation:
Assignee:
MICROMASS LTD (GB)
BROWN JEFFERY MARK (GB)
BROWN JEFFERY MARK (GB)
International Classes:
G01N27/64; H01J49/00
Domestic Patent References:
| WO2010120895A1 | 2010-10-21 |
Other References:
KASPER D. RAND ET AL: "Gas-Phase Hydrogen/Deuterium Exchange in a Traveling Wave Ion Guide for the Examination of Protein Conformations", ANALYTICAL CHEMISTRY, vol. 81, no. 24, 15 December 2009 (2009-12-15), pages 10019 - 10028, XP055004594, ISSN: 0003-2700, DOI: 10.1021/ac901897x
ROBINSON E W ET AL: "Multidimensional Separations of Ubiquitin Conformers in the Gas Phase: Relating Ion Cross Sections to H/D Exchange Measurements", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 16, no. 9, 1 September 2005 (2005-09-01), pages 1427 - 1437, XP025302924, ISSN: 1044-0305, [retrieved on 20050901], DOI: 10.1016/J.JASMS.2005.04.007
STEPHEN J. VALENTINE ET AL: "H/D Exchange Levels of Shape-Resolved Cytochrome c Conformers in the Gas Phase", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 119, no. 15, 1 April 1997 (1997-04-01), pages 3558 - 3566, XP055004672, ISSN: 0002-7863, DOI: 10.1021/ja9626751
KORNÉL NAGY ET AL: "Online Hydrogen/Deuterium Exchange Performed in the Ion Mobility Cell of a Hybrid Mass Spectrometer", ANALYTICAL CHEMISTRY, vol. 81, no. 22, 15 November 2009 (2009-11-15), pages 9365 - 9371, XP055004599, ISSN: 0003-2700, DOI: 10.1021/ac901736j
RANDY W PURVES ET AL: "Combining H-D exchange and ESI-FAIMS-MS for detecting gas-phase conformers of equine cytochrome c", CANADIAN JOURNAL OF CHEMISTRY, vol. 83, 27 January 2006 (2006-01-27), pages 1961 - 1968, XP008140903, DOI: 10.1139/v05-215
ROBINSON E W ET AL: "Multidimensional Separations of Ubiquitin Conformers in the Gas Phase: Relating Ion Cross Sections to H/D Exchange Measurements", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 16, no. 9, 1 September 2005 (2005-09-01), pages 1427 - 1437, XP025302924, ISSN: 1044-0305, [retrieved on 20050901], DOI: 10.1016/J.JASMS.2005.04.007
STEPHEN J. VALENTINE ET AL: "H/D Exchange Levels of Shape-Resolved Cytochrome c Conformers in the Gas Phase", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 119, no. 15, 1 April 1997 (1997-04-01), pages 3558 - 3566, XP055004672, ISSN: 0002-7863, DOI: 10.1021/ja9626751
KORNÉL NAGY ET AL: "Online Hydrogen/Deuterium Exchange Performed in the Ion Mobility Cell of a Hybrid Mass Spectrometer", ANALYTICAL CHEMISTRY, vol. 81, no. 22, 15 November 2009 (2009-11-15), pages 9365 - 9371, XP055004599, ISSN: 0003-2700, DOI: 10.1021/ac901736j
RANDY W PURVES ET AL: "Combining H-D exchange and ESI-FAIMS-MS for detecting gas-phase conformers of equine cytochrome c", CANADIAN JOURNAL OF CHEMISTRY, vol. 83, 27 January 2006 (2006-01-27), pages 1961 - 1968, XP008140903, DOI: 10.1139/v05-215
Attorney, Agent or Firm:
JEFFREY, Philip (St Bride's House10 Salisbury Square, London EC4Y 8JD, GB)
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