Title:
MATERIALS, METHODS AND DEVICES FOR STIMULATING AND DIRECTING THE PROLIFERATION AND MIGRATION OF NEURAL PROGENITORS AND AXONAL AND DENDRITIC GROWTH
Document Type and Number:
WIPO Patent Application WO/2014/096489
Kind Code:
A3
Abstract:
The present invention consists of compositions, methods and devices for inducing, promoting and directing adherence, proliferation and migration of neural precursors and axonal and dendritic growth. It comprises electro-conductive substrates comprising of an electro-conductive polymer doped with polystyrene sulfonate-maleic acid copolymer (PSS-co-MA) forming biofunctionalized surfaces of microfibers for presenting one or several biomolecules of interest in order to control cell behaviour. These substrates may be used for neurological studies, cell cultures and pharmagological assays in vitro; as well as for being implanted in the nervous system in the form of scaffolds or electro-biological devices, so as to promote neural tissue repair or to improve communication in the electrode/neural cell interface in neuroprosthetic systems. The cellular effects are attained by the type, number or way of molecule binding to the substrate, by the physical properties thereof and geometry of scaffolds, by electric stimulation or by the combined action of these factors.
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Inventors:
COLLAZOS CASTRO JORGE EDUARDO (ES)
POLO SANZ JOSÉ LUIS (ES)
HERNÁNDEZ LABRADO GABRIEL RAÚL (ES)
GARCÍA RAMA PACHECO CONCEPCIÓN (ES)
POLO SANZ JOSÉ LUIS (ES)
HERNÁNDEZ LABRADO GABRIEL RAÚL (ES)
GARCÍA RAMA PACHECO CONCEPCIÓN (ES)
Application Number:
PCT/ES2013/070879
Publication Date:
August 14, 2014
Filing Date:
December 16, 2013
Export Citation:
Assignee:
UNIV CASTILLA LA MANCHA (ES)
FUNDACIÓN HOSPITAL NAC DE PARAPLÉJICOS PARA LA INVESTIGACIÓN Y LA INTEGRACIÓN FUHNPAIIN (ES)
FUNDACIÓN HOSPITAL NAC DE PARAPLÉJICOS PARA LA INVESTIGACIÓN Y LA INTEGRACIÓN FUHNPAIIN (ES)
International Classes:
G01N33/543; A61L27/00; A61L31/00; C12M1/12; C12M3/00
Domestic Patent References:
| WO2006127712A2 | 2006-11-30 |
Foreign References:
| KR20120073855A | 2012-07-05 |
Other References:
JORGE E. COLLAZOS-CASTRO ET AL: "Bioelectrochemical control of neural cell development on conducting polymers", BIOMATERIALS, vol. 31, no. 35, 1 December 2010 (2010-12-01), pages 9244 - 9255, XP055112742, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2010.08.057
CUI X ET AL: "Electrochemical deposition and characterization of poly(3,4-ethylenedioxythiophene) on neural microelectrode arrays", SENSORS AND ACTUATORS B: CHEMICAL: INTERNATIONAL JOURNAL DEVOTED TO RESEARCH AND DEVELOPMENT OF PHYSICAL AND CHEMICAL TRANSDUCERS, ELSEVIER S.A, CH, vol. 89, no. 1-2, 1 March 2003 (2003-03-01), pages 92 - 102, XP004409471, ISSN: 0925-4005, DOI: 10.1016/S0925-4005(02)00448-3
A.-D. BENDREA ET AL: "Review paper: Progress in the Field of Conducting Polymers for Tissue Engineering Applications", JOURNAL OF BIOMATERIALS APPLICATIONS, vol. 26, no. 1, 16 June 2011 (2011-06-16), pages 3 - 84, XP055113540, ISSN: 0885-3282, DOI: 10.1177/0885328211402704
JORGE E. COLLAZOS-CASTRO ET AL: "N-Cadherin- and L1-functionalised conducting polymers for synergistic stimulation and guidance of neural cell growth", BIOMATERIALS, vol. 34, no. 14, 1 May 2013 (2013-05-01), pages 3603 - 3617, XP055113464, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2013.01.097
LEE J Y ET AL: "Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications", BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 30, no. 26, 1 September 2009 (2009-09-01), pages 4325 - 4335, XP026393767, ISSN: 0142-9612, [retrieved on 20090607]
KARL SVENNERSTEN ET AL: "Organic bioelectronics in nanomedicine", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - GENERAL SUBJECTS, ELSEVIER, AMSTERDAM, NL, vol. 1810, no. 3, 1 October 2010 (2010-10-01), pages 276 - 285, XP028136081, ISSN: 0304-4165, [retrieved on 20101008], DOI: 10.1016/J.BBAGEN.2010.10.001
CUI X ET AL: "Electrochemical deposition and characterization of poly(3,4-ethylenedioxythiophene) on neural microelectrode arrays", SENSORS AND ACTUATORS B: CHEMICAL: INTERNATIONAL JOURNAL DEVOTED TO RESEARCH AND DEVELOPMENT OF PHYSICAL AND CHEMICAL TRANSDUCERS, ELSEVIER S.A, CH, vol. 89, no. 1-2, 1 March 2003 (2003-03-01), pages 92 - 102, XP004409471, ISSN: 0925-4005, DOI: 10.1016/S0925-4005(02)00448-3
A.-D. BENDREA ET AL: "Review paper: Progress in the Field of Conducting Polymers for Tissue Engineering Applications", JOURNAL OF BIOMATERIALS APPLICATIONS, vol. 26, no. 1, 16 June 2011 (2011-06-16), pages 3 - 84, XP055113540, ISSN: 0885-3282, DOI: 10.1177/0885328211402704
JORGE E. COLLAZOS-CASTRO ET AL: "N-Cadherin- and L1-functionalised conducting polymers for synergistic stimulation and guidance of neural cell growth", BIOMATERIALS, vol. 34, no. 14, 1 May 2013 (2013-05-01), pages 3603 - 3617, XP055113464, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2013.01.097
LEE J Y ET AL: "Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications", BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 30, no. 26, 1 September 2009 (2009-09-01), pages 4325 - 4335, XP026393767, ISSN: 0142-9612, [retrieved on 20090607]
KARL SVENNERSTEN ET AL: "Organic bioelectronics in nanomedicine", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - GENERAL SUBJECTS, ELSEVIER, AMSTERDAM, NL, vol. 1810, no. 3, 1 October 2010 (2010-10-01), pages 276 - 285, XP028136081, ISSN: 0304-4165, [retrieved on 20101008], DOI: 10.1016/J.BBAGEN.2010.10.001
Attorney, Agent or Firm:
PONS ARIÑO, Ángel (Glorieta Rubén Darío 4, Madrid, ES)
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