Title:
MEANS AND METHODS FOR PRODUCING NEISSERIA MENINGITIDIS CAPSULAR POLYSACCHARIDES OF LOW DISPERSITY
Document Type and Number:
WIPO Patent Application WO/2015/158898
Kind Code:
A3
Abstract:
The present invention relates to in vitro methods for producing Neisseria meningitidis capsular polysaccharides which have a defined length. The present invention also relates to compositions comprising at least one capsule polymerase, at least one donor carbohydrate and at least one acceptor carbohydrate, wherein the ratio of donor carbohydrate to acceptor carbohydrate is a ratio from 10:1 to 400:1. Moreover, the present invention provides truncated versions of the capsule polymerases of Neisseria meningitidis serogroups A and X. Also provided herein are pharmaceuticals, in particular vaccines, comprising the synthetic capsular polysaccharides of Neisseria meningitidis which have a defined length. Furthermore, the invention provides for methods for the production of said vaccines.
Inventors:
FIEBIG TIMM (DE)
KEYS TIM (CH)
FREIBERGER FRIEDRICH (DE)
ROMANOW ANGELA (DE)
BETHE ANDREA (DE)
BERGER MONIKA (DE)
GERARDY-SCHAHN RITA (DE)
KEYS TIM (CH)
FREIBERGER FRIEDRICH (DE)
ROMANOW ANGELA (DE)
BETHE ANDREA (DE)
BERGER MONIKA (DE)
GERARDY-SCHAHN RITA (DE)
Application Number:
PCT/EP2015/058399
Publication Date:
December 10, 2015
Filing Date:
April 17, 2015
Export Citation:
Assignee:
HANNOVER MED HOCHSCHULE (DE)
International Classes:
A61K39/095; C08B37/00; C12N1/20; C12P19/00; C12P19/18
Domestic Patent References:
| WO2011023764A1 | 2011-03-03 | |||
| WO2013174832A1 | 2013-11-28 | |||
| WO2007000322A1 | 2007-01-04 | |||
| WO2008011201A2 | 2008-01-24 |
Foreign References:
| US20060073168A1 | 2006-04-06 |
Other References:
CLAUS HEIKE ET AL: "Amino acid 310 determines the donor substrate specificity of serogroup W-135 and Y capsule polymerases of Neisseria meningitidis", MOLECULAR MICROBIOLOGY, WILEY BLACKWELL - OXFORD : BLACKWELL, 1987-, US, vol. 71, no. 4, 1 February 2009 (2009-02-01), pages 960 - 971, XP009141601, ISSN: 0950-382X, [retrieved on 20081223], DOI: 10.1111/J.1365-2958.2008.06580.X
GRIFFISS J M ET AL: "Safety and immunogenicity of group Y and group W135 meningococcal capsular polysaccharide vaccines in adults", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 34, no. 3, 1 December 1981 (1981-12-01), pages 725 - 732, XP002611797, ISSN: 0019-9567
F NATO ET AL: "Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents", JOURNAL OF CLINICAL MICROBIOLOGY, 1 July 1991 (1991-07-01), UNITED STATES, pages 1447 - 1452, XP055195025, Retrieved from the Internet
GRIFFISS J M ET AL: "Safety and immunogenicity of group Y and group W135 meningococcal capsular polysaccharide vaccines in adults", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 34, no. 3, 1 December 1981 (1981-12-01), pages 725 - 732, XP002611797, ISSN: 0019-9567
F NATO ET AL: "Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents", JOURNAL OF CLINICAL MICROBIOLOGY, 1 July 1991 (1991-07-01), UNITED STATES, pages 1447 - 1452, XP055195025, Retrieved from the Internet
Attorney, Agent or Firm:
MEIER, Jürgen (Patentanwälte Rechtsanwälte mbBSiebertstraße 3, München, DE)
Download PDF: