Title:
MEASUREMENT OF ANTI-TREPONEMA PALLIDUM BODY FLUID ANTIBODY USING TREPONEMA PALLIDUM BACTERIUM SURFACE ANTIGEN MIXTURE
Document Type and Number:
WIPO Patent Application WO/2022/259719
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a means of detecting infection with Treponema pallidum bacterium at a higher sensitivity. The present inventor found that, in the measurement of anti-T. pallidum antibody in a body fluid specimen by an immunoassay method, the assay sensitivity is obviously increased at a practical level, beyond the level observed using native T. pallidum bacterium antigen, by using a mixture of TpN47 antigen derived from the silk thread or silk gland of a recombinant silkworm, said antigen being an artificial surface antigen of T. pallidum bacterium, and genetically modified TpN15-TpN17 fusion antigen, said antigen also being an artificial surface antigen of T. pallidum bacterium.
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Inventors:
HASEGAWA MUNECHIKA (JP)
Application Number:
PCT/JP2022/014979
Publication Date:
December 15, 2022
Filing Date:
March 28, 2022
Export Citation:
Assignee:
SETEKA CORP (JP)
International Classes:
C07K17/02; C12N15/31; G01N33/53; G01N33/531; G01N33/543; G01N33/571
Foreign References:
| JPH09288110A | 1997-11-04 | |||
| JP2001228151A | 2001-08-24 | |||
| JPH11192089A | 1999-07-21 |
Other References:
ANONYMOUS: "Sales of Recombinant Silkworm Syphilis (TP) Antigen Started (Certificate of Compliance and Analysis COA: Product Nameļ¼TpN47 Recombinant antigen // Product Nameļ¼Recombinant fusion protein of TpN15 and TpN17)", NOTICE. RIMCO CO., LTD., RIMCO CO., LTD., JP, JP, pages 1 - 2, XP009541932, Retrieved from the Internet [retrieved on 20220530]
SAMBRI V, MARANGONI A, SIMONE M A, D 'ANTUONO A, NEGOSANTI M, CEVENINI R: "Evaluation of recomWell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis", CLINICAL MICROBIOLOGY AND INFECTION, ELSEVIER LTD, GB, vol. 7, no. 4, 30 April 2001 (2001-04-30), GB , pages 200 - 205, XP093013348, ISSN: 1469-0691, DOI: 10.1046/j.1469-0691.2001.00232.x
SAMBRI V, MARANGONI A, SIMONE M A, D 'ANTUONO A, NEGOSANTI M, CEVENINI R: "Evaluation of recomWell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis", CLINICAL MICROBIOLOGY AND INFECTION, ELSEVIER LTD, GB, vol. 7, no. 4, 30 April 2001 (2001-04-30), GB , pages 200 - 205, XP093013348, ISSN: 1469-0691, DOI: 10.1046/j.1469-0691.2001.00232.x
Attorney, Agent or Firm:
SHIMURA Mitsuharu (JP)
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