Title:
MECHANISM OF SEPARATING AND PURIFYING DNA AND SO ON
Document Type and Number:
WIPO Patent Application WO/2005/078088
Kind Code:
A1
Abstract:
A simplified mechanism and method for highly efficiently separating and purifying a nucleic acid (in particular a fragmental DNA) at a high reproducibility without resort to elution with a salt at a high concentration or elution/purification, thereby giving a fragment having a high purity. This mechanism, which aims at purifying a nucleic acid (in particular a fragmental DNA) with the use of a monolith structure, is provided with a monolith structure made of glass or silica, namely, a porous integral structure having small pores penetrating thorough from the top to the bottom and the penetrating pores being in a size fitting for the nucleic acid size of from 35 bp (mer) to 100 Kbp (mer).
Inventors:
ABUDOKIRIM ABUDOGUPUR (JP)
OHIRA MASAYOSHI (JP)
OKUSA KENSUKE (JP)
SETO NOBUO (JP)
FURUNO MASAHIRO (JP)
OHIRA MASAYOSHI (JP)
OKUSA KENSUKE (JP)
SETO NOBUO (JP)
FURUNO MASAHIRO (JP)
Application Number:
PCT/JP2004/001465
Publication Date:
August 25, 2005
Filing Date:
February 12, 2004
Export Citation:
Assignee:
GL SCIENCES INC (JP)
ABUDOKIRIM ABUDOGUPUR (JP)
OHIRA MASAYOSHI (JP)
OKUSA KENSUKE (JP)
SETO NOBUO (JP)
FURUNO MASAHIRO (JP)
ABUDOKIRIM ABUDOGUPUR (JP)
OHIRA MASAYOSHI (JP)
OKUSA KENSUKE (JP)
SETO NOBUO (JP)
FURUNO MASAHIRO (JP)
International Classes:
C07H21/00; C12N15/10; (IPC1-7): C12N15/10; C07H21/00; C12P19/34
Other References:
ALES PODGORNIK, ET AL: "Application of very short monolithic columns for separation of low and high molecular mass substances", JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol. 25, no. 20, 2002, pages 3099 - 3116, XP002976459
LUBBAD S, ET AL: "Micropreparative fractionation of DNA fragments on metathesis-based monoliths: influence of stoichiometry on separation", J CHROMATOGR A., vol. 959, no. 1-2, 2002, pages 121 - 129, XP004360281
WALCHER W, ET AL: "Monolithic capillary columns for liquid chromatography-electrospray ionization mass spectrometry in proteomic and genomic research", J CHROMATOGR B ANALYT TECHNOL BIOMED LIFE SCI., vol. 782, no. 1-2, 2002, pages 111 - 125, XP004394220
LUBBAD S, ET AL: "Micropreparative fractionation of DNA fragments on metathesis-based monoliths: influence of stoichiometry on separation", J CHROMATOGR A., vol. 959, no. 1-2, 2002, pages 121 - 129, XP004360281
WALCHER W, ET AL: "Monolithic capillary columns for liquid chromatography-electrospray ionization mass spectrometry in proteomic and genomic research", J CHROMATOGR B ANALYT TECHNOL BIOMED LIFE SCI., vol. 782, no. 1-2, 2002, pages 111 - 125, XP004394220
Attorney, Agent or Firm:
Takahashi, Mitsuo (Shinjukunishi Blg. 11-17, Nishishinjuku 7-chom, Shinjuku-ku Tokyo, JP)
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