Title:
MEDICINAL PRODUCT FOR TISSUE REGENERATION, AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2019/093481
Kind Code:
A1
Abstract:
The present invention pertains to a cell-based medicinal product containing mesenchymal stem cells, and a preparation method therefor. The present invention specifically pertains to a cell-based medicinal product containing mesenchymal stem cells, wherein: a) the mesenchymal stem cells express CX3CL1 in response to an inflammatory cytokine stimulus; and/or b) at least 90% of the mesenchymal stem cells express EGFR and/or ITGA4. The present invention also specifically pertains to a preparation method for a cell-based medicinal product containing mesenchymal stem cells, the method including: a) a step for adding inflammatory cytokines to a culture containing mesenchymal stem cells, and confirming that the mesenchymal stem cells express CX3CL1; and/or b) a step for confirming that at least 90% of the mesenchymal stem cells express EGFR and/or ITGA4.
Inventors:
HONMOU OSAMU (JP)
YOSHIKAWA YOSHIHIRO (JP)
TOMII RYO (JP)
YAO MASAFUMI (JP)
NISHII YUKARI (JP)
WAGATSUMA YUSUKE (JP)
YOSHIKAWA YOSHIHIRO (JP)
TOMII RYO (JP)
YAO MASAFUMI (JP)
NISHII YUKARI (JP)
WAGATSUMA YUSUKE (JP)
Application Number:
PCT/JP2018/041678
Publication Date:
May 16, 2019
Filing Date:
November 09, 2018
Export Citation:
Assignee:
UNIV SAPPORO MEDICAL (JP)
NIPRO CORP (JP)
NIPRO CORP (JP)
International Classes:
A61K35/28; A61P17/02; A61P25/00; A61P37/02; C12N5/0775; C12Q1/6806; G01N33/15; G01N33/50; G01N33/68
Domestic Patent References:
| WO2017170925A1 | 2017-10-05 | |||
| WO2009034708A1 | 2009-03-19 | |||
| WO2005094888A1 | 2005-10-13 | |||
| WO2018159432A1 | 2018-09-07 | |||
| WO2002000849A1 | 2002-01-03 | |||
| WO2009002503A1 | 2008-12-31 | |||
| WO2009034708A1 | 2009-03-19 |
Foreign References:
| JP2011525798A | 2011-09-29 | |||
| JP2009506769A | 2009-02-19 | |||
| JP2017520537A | 2017-07-27 |
Other References:
DU, W. J. ET AL.: "Heterogeneity of proangiogenic features in mesenchymal stem cells derived from bone marrow, adipose tissue, umbilical cord, and placenta", STEM CELL RESEARCH & THERAPY, vol. 7, no. 1, 2016, pages 163, XP055606861
MEAD, B. ET AL.: "Paracrine-mediated neuroprotection and neuritogenesis of axotomised retinal ganglion cells by human dental pulp stem cells: comparison with human bone marrow and adipose-derived mesenchymal stem cells", PLOS ONE, vol. 9, no. 10, e109305, 7 October 2014 (2014-10-07), pages 1 - 11, XP055606867
GIUNTI, D. ET AL.: "Mesenchymal stem cells shape microglia effector functions through the release of CX3CL1", STEM CELLS, vol. 30, no. 9, 20 July 2012 (2012-07-20), pages 2044 - 2053, XP055606871
CROITORU-LAMOURY, J. ET AL.: "Human Mesenchymal Stem Cells Constitutively Express Chemokines and Chemokine Receptors That Can Be Upregulated by Cytokines, IFN-beta, and Copaxone", JOURNAL OF INTERFERON & CYTOKINE RESEARCH, vol. 27, no. 1, 2007, pages 53 - 64, XP055320913, DOI: doi:10.1089/jir.2006.0037
KUMAR, S. ET AL.: "Bone homing of mesenchymal stem cells by ectopic alpha4 integrin expression", FASEB JOURNAL, vol. 21, no. 14, 2007, pages 3917 - 3927, XP055099889, DOI: doi:10.1096/fj.07-8275com
CUI, L. L. ET AL.: "Integrin alpha4 Overexpression on Rat Mesenchymal Stem Cells Enhances Transmigration and Reduces Cerebral Embolism After Intracarotid Injection", STROKE, vol. 48, no. 10, October 2017 (2017-10-01), pages 2895 - 2900, XP055606877
IIHOSHI S. ET AL., BRAIN RES., vol. 1007, 2004, pages 1 - 9
NOMURA T. ET AL., NEUROSCIENCE, vol. 136, 2005, pages 161 - 169
HONMA T. ET AL., EXP. NEUROL., vol. 199, 2006, pages 56 - 66
HONMOU 0. ET AL., BRAIN, vol. 134, 2011, pages 1790 - 1807
HERVEY ET AL., BRAIN RESARCH, vol. 1619, 2015, pages 36 - 71
OSAKA ET AL., BRAIN RESEARCH, vol. 1343, 2010, pages 226 - 235
SASAKI ET AL., JOURNAL OF NEUROSCIENCE, vol. 29, no. 47, 2009, pages 14932 - 14941
GIUNTI ET AL., STEM CELLS, vol. 30, 2012, pages 2044 - 53
YOO ET AL., NEUROBIOLOGY OF DISEASE, vol. 58, 2013, pages 249 - 257
LIU ET AL., JOURNAL OF NEUROINFLAMMATION, vol. 11, 2014, pages 135
NOH ET AL., STEM CELLS TRANSLATOINAL MEDICINE, vol. 5, 2016, pages 1 - 12
NAKAJIMA ET AL., JOURNAL OF NEUROTRAUMA, vol. 29, 2012, pages 1614 - 25
ONO ET AL., BURNS, vol. 21, 1995, pages 352 - 355
ZHUANG ET AL., ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2013, pages 383 - 386
WERNER ET AL., PHYSIOL REV, vol. 83, 2003, pages 835 - 870
See also references of EP 3708175A4
MEAD, B. ET AL.: "Paracrine-mediated neuroprotection and neuritogenesis of axotomised retinal ganglion cells by human dental pulp stem cells: comparison with human bone marrow and adipose-derived mesenchymal stem cells", PLOS ONE, vol. 9, no. 10, e109305, 7 October 2014 (2014-10-07), pages 1 - 11, XP055606867
GIUNTI, D. ET AL.: "Mesenchymal stem cells shape microglia effector functions through the release of CX3CL1", STEM CELLS, vol. 30, no. 9, 20 July 2012 (2012-07-20), pages 2044 - 2053, XP055606871
CROITORU-LAMOURY, J. ET AL.: "Human Mesenchymal Stem Cells Constitutively Express Chemokines and Chemokine Receptors That Can Be Upregulated by Cytokines, IFN-beta, and Copaxone", JOURNAL OF INTERFERON & CYTOKINE RESEARCH, vol. 27, no. 1, 2007, pages 53 - 64, XP055320913, DOI: doi:10.1089/jir.2006.0037
KUMAR, S. ET AL.: "Bone homing of mesenchymal stem cells by ectopic alpha4 integrin expression", FASEB JOURNAL, vol. 21, no. 14, 2007, pages 3917 - 3927, XP055099889, DOI: doi:10.1096/fj.07-8275com
CUI, L. L. ET AL.: "Integrin alpha4 Overexpression on Rat Mesenchymal Stem Cells Enhances Transmigration and Reduces Cerebral Embolism After Intracarotid Injection", STROKE, vol. 48, no. 10, October 2017 (2017-10-01), pages 2895 - 2900, XP055606877
IIHOSHI S. ET AL., BRAIN RES., vol. 1007, 2004, pages 1 - 9
NOMURA T. ET AL., NEUROSCIENCE, vol. 136, 2005, pages 161 - 169
HONMA T. ET AL., EXP. NEUROL., vol. 199, 2006, pages 56 - 66
HONMOU 0. ET AL., BRAIN, vol. 134, 2011, pages 1790 - 1807
HERVEY ET AL., BRAIN RESARCH, vol. 1619, 2015, pages 36 - 71
OSAKA ET AL., BRAIN RESEARCH, vol. 1343, 2010, pages 226 - 235
SASAKI ET AL., JOURNAL OF NEUROSCIENCE, vol. 29, no. 47, 2009, pages 14932 - 14941
GIUNTI ET AL., STEM CELLS, vol. 30, 2012, pages 2044 - 53
YOO ET AL., NEUROBIOLOGY OF DISEASE, vol. 58, 2013, pages 249 - 257
LIU ET AL., JOURNAL OF NEUROINFLAMMATION, vol. 11, 2014, pages 135
NOH ET AL., STEM CELLS TRANSLATOINAL MEDICINE, vol. 5, 2016, pages 1 - 12
NAKAJIMA ET AL., JOURNAL OF NEUROTRAUMA, vol. 29, 2012, pages 1614 - 25
ONO ET AL., BURNS, vol. 21, 1995, pages 352 - 355
ZHUANG ET AL., ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2013, pages 383 - 386
WERNER ET AL., PHYSIOL REV, vol. 83, 2003, pages 835 - 870
See also references of EP 3708175A4
Attorney, Agent or Firm:
UMEDA Shinsuke et al. (JP)
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