Title:
MEDIUM-FILLING SOLUTION, MEDIUM FILLING METHOD, CULTURE CONTAINER, AND BUBBLE-REMOVING APPARATUS FOR FILLING MEDIUM
Document Type and Number:
WIPO Patent Application WO/2020/100567
Kind Code:
A1
Abstract:
According to the present invention, when cells are cultured using a culture container having fine recessed portions, bubbles collected in the fine recessed portions can be effectively removed, which can be applied to a culture container having a large culture surface. This medium filling solution is a solution in which when a medium is filled into a culture container having a culture surface in which a plurality of fine recessed portions are formed, the bubbles collected in the fine recessed portions are removed prior to the filling of the medium. This medium-filling solution is filled into the culture container having the culture surface in which the plurality of fine recessed portions are formed and is left standing for a certain period of time, a medium is then passed through the culture container, and the medium-filling solution is substituted with the medium.
Inventors:
KOSEKI OSAMU (JP)
TANAKA SATOSHI (JP)
MITSUISHI MAKI (JP)
TANAKA SATOSHI (JP)
MITSUISHI MAKI (JP)
Application Number:
PCT/JP2019/042243
Publication Date:
May 22, 2020
Filing Date:
October 29, 2019
Export Citation:
Assignee:
TOYO SEIKAN GROUP HOLDINGS LTD (JP)
International Classes:
C12M1/00; C12M3/00; C12N1/00; F04B37/16; C12N5/10
Domestic Patent References:
| WO2017170335A1 | 2017-10-05 |
Foreign References:
| JP2018013278A | 2018-01-25 | |||
| JP2015213497A | 2015-12-03 | |||
| JP2005507492A | 2005-03-17 | |||
| US20080145908A1 | 2008-06-19 | |||
| JP2015043751A | 2015-03-12 | |||
| US20160222336A1 | 2016-08-04 |
Other References:
MIWA, AYA ET AL.: "Study on Effects of Pressure and Duration of Ultrasound Pulse on Pulsation of Cultured Cardiomyocytes", 2016 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS, 2016, pages 1 - 4, XP032988323, DOI: 10.1109/ULTSYM.2016.7728441
Attorney, Agent or Firm:
IKUTOMI Shigekazu (JP)
Download PDF: