Title:
MEDIUM MICROBALLOON-BASED FLORESCENCE CORRELATION SPECTRUM ANALYSIS METHOD AND DEVICE
Document Type and Number:
WIPO Patent Application WO/2013/020315
Kind Code:
A1
Abstract:
A medium microballoon-based florescence correlation spectrum analysis method capable of being effectively applied to high concentration florescence molecular samples. Radial polarization light (R1) and tangential polarization light (R2) are used as a florescence excitation beam and a florescence suppression beam respectively, and are further applied to a florescence sample (3) and excite a florescence signal after successively being subjecting to focusing by a microscope objective (1) and the nanometric injection of a medium microballoon (2), and florescence correlation spectrum analysis is completed by collecting and analyzing the florescence signal. The corresponding devices successively include: a light source generating radial polarization light (R1) and tangential polarization light (R2), a first microscope objective (1), a medium microballoon (2), a sample shelf (5) where a florescence sample (3) is placed, and a second microscope objective (4), and further include a florescence signal analysis and processing device connected to the second microscope objective. The first microscope objective (1), medium microballoon (2), florescence sample (3) and second microscope objective (4) are all on the coaxial light path of the radial polarization light (R1) and tangential polarization light (R2), and the medium microballoon (2) is located on the object space focal plane of the first microscope objective (1).
Inventors:
KUANG CUIFANG (CN)
HAO XIANG (CN)
LIU XU (CN)
WANG TINGTING (CN)
HAO XIANG (CN)
LIU XU (CN)
WANG TINGTING (CN)
Application Number:
PCT/CN2011/079365
Publication Date:
February 14, 2013
Filing Date:
September 06, 2011
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
KUANG CUIFANG (CN)
HAO XIANG (CN)
LIU XU (CN)
WANG TINGTING (CN)
KUANG CUIFANG (CN)
HAO XIANG (CN)
LIU XU (CN)
WANG TINGTING (CN)
International Classes:
G01N21/64; G01J3/28; G02B21/00
Domestic Patent References:
| WO2005068979A1 | 2005-07-28 | |||
| WO2006133944A1 | 2006-12-21 |
Foreign References:
| CN101907766A | 2010-12-08 | |||
| CN101382663A | 2009-03-11 | |||
| CN101387759A | 2009-03-18 |
Attorney, Agent or Firm:
HANGZHOU TIANQIN INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
杭州天勤知识产权代理有限公司 (CN)
杭州天勤知识产权代理有限公司 (CN)
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Claims:
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