MEDIUM FOR PREPARING FEEDER CELLS FOR EMBRYONIC STEM CELLS AND FEEDER CELLS

Title:

MEDIUM FOR PREPARING FEEDER CELLS FOR EMBRYONIC STEM CELLS AND FEEDER CELLS

Document Type and Number:

WIPO Patent Application WO/2005/078070

Kind Code:

A1

Abstract:

It is intended to efficiently establish a feeder cell usable in culturing embryonic stem cells including human embryonic stem cells from a restricted donor-origin material to thereby provide a medium for preparing feeder cells for embryonic stem cells enabling culture with little risk of infection. It is also intended to provide a method of producing feeder cells which are relatively safe in the case of culturing together with embryonic stem cells including human embryonic stem cells, and feeder cells thus obtained. By using a medium for preparing feeder cells for embryonic stem cells obtained by adding at least serum albumin and insulin to a basal medium, it is possible to culture, in a stable state, a mass of cells including at least one type of cells available as feeder cells for embryonic stem cells which are selected from among fetal epidermal fibroblasts, fetal muscular fibroblasts, fetal pulmonary fibroblasts, fetal epithelial cells, fetal endothelial cells, adult epidermal fibroblasts, adult pulmonary fibroblasts, adult epithelial fibroblasts and adult epidermal fibroblasts.

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Inventors:

NAKATSUJI NORIO (JP)
SUEMORI HIROFUMI (JP)
ASAKA ISAO (JP)
SUGAI HARUMI (JP)
OKAMOTO REIKO (JP)

Application Number:

PCT/JP2005/002027

Publication Date:

August 25, 2005

Filing Date:

February 10, 2005

Export Citation:

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Assignee:

REPROCELL INC (JP)
ASAHI TECHNO GLASS CORP (JP)
TANABE SEIYAKU CO (JP)
NAKATSUJI NORIO (JP)
SUEMORI HIROFUMI (JP)
ASAKA ISAO (JP)
SUGAI HARUMI (JP)
OKAMOTO REIKO (JP)

International Classes:

C12N5/00; C12N5/0735; C12N5/077; (IPC1-7): C12N5/06; A61K35/36; A61K48/00; A61L27/00

Domestic Patent References:

WO2002029012A1

2002-04-11

Foreign References:

JP2003525625A	2003-09-02
JP2001508302A	2001-06-26
JP2003009854A	2003-01-14
JPH078273A	1995-01-13

Other References: