Title:
MEDIUM FOR THE REDIFFERENTIATION OF DEDIFFERENTIATED CHONDROCYTES INTO CHONDROCYTES
Document Type and Number:
WIPO Patent Application WO/2006/022263
Kind Code:
A1
Abstract:
It is intended to provide a medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes and a method of redifferentiating dedifferentiated chondrocytes into chondrocytes whereby dedifferentiated chondrocytes, which have been dedifferentiated by, for example, culturing in vitro and thus show weakened chondral characteristics, can be efficiently and easily redifferentiated into the original chondrocytes. That is to say, a medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes which is to be used for redifferentiating dedifferentiated chondrocytes having been dedifferentiated and thus showing weakened chondral characteristics into the original chondrocytes and contains insulin together with at least one member selected from among BMP-2 and its analogs; and a method of redifferentiating dedifferentiated chondrocytes into the original chondrocytes by culturing the dedifferentiated chondrocytes with the use of the medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes. In a preferred embodiment, the above-described medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes further contains T3.
Inventors:
HOSHI KAZUTO (JP)
TEI YUICHI (JP)
KAWAGUCHI HIROSHI (JP)
NAKAMURA KOZO (JP)
TAKADO TAKESHI (JP)
TEI YUICHI (JP)
KAWAGUCHI HIROSHI (JP)
NAKAMURA KOZO (JP)
TAKADO TAKESHI (JP)
Application Number:
PCT/JP2005/015280
Publication Date:
March 02, 2006
Filing Date:
August 23, 2005
Export Citation:
Assignee:
HOSHI KAZUTO (JP)
TEI YUICHI (JP)
KAWAGUCHI HIROSHI (JP)
NAKAMURA KOZO (JP)
TAKADO TAKESHI (JP)
TEI YUICHI (JP)
KAWAGUCHI HIROSHI (JP)
NAKAMURA KOZO (JP)
TAKADO TAKESHI (JP)
International Classes:
C12N5/00; A61K35/32; A61L27/00; A61L27/38; A61P19/00; C12N5/02; C12N5/07; C12N5/071; C12N5/077
Domestic Patent References:
| WO2003064598A2 | 2003-08-07 |
Other References:
QUARTO R. ET AL.: "Thyroid hormone, insulin, and glucocorticoids are sufficient to support chondrocyte differentiation t hyertrophy:a serum-free analysis.", J.CELL BIOL., vol. 19, no. 4, November 1992 (1992-11-01), pages 989 - 995, XP002993351
ALINI M. ET AL.: "In serum-free culture thyroid hormones can induce full expression of chondrocyte hypertrophy leading to matrix calcification.", J. BONE MINERAL RES., vol. 11, no. 1, 1996, pages 105 - 113, XP001131985
LEBOY P. ET AL.: "Rapid chondrocyte aturation by serum-free culture with BMP-2 and ascorbic acid.", J. CELL. BIOCHEM., vol. 66, 1997, pages 394 - 403, XP002993352
CHEN G. ET AL.: "Redifferentation of dediferentiated bovine chondrocytes when cultured in vitro on a PLGA-collagen hybrid mesh.", FEBS LETT., vol. 542, 2003, pages 95 - 99, XP004422730
PEI M. ET AL.: "Growth factors for sequential cellular de-and re-differentiation in tissue engineering.", BIOCHEM.BIOPHYS. RES.COMMUN., vol. 294, 2002, pages 149 - 154, XP002993353
SHUM L. ET AL.: "BMP4 promotes chondrocyte proliferation and hypertrophy in the endochondral cranial base.", INT.J.DEV.BIOL., vol. 47, 2003, pages 423 - 431, XP002993354
See also references of EP 1792977A4
ALINI M. ET AL.: "In serum-free culture thyroid hormones can induce full expression of chondrocyte hypertrophy leading to matrix calcification.", J. BONE MINERAL RES., vol. 11, no. 1, 1996, pages 105 - 113, XP001131985
LEBOY P. ET AL.: "Rapid chondrocyte aturation by serum-free culture with BMP-2 and ascorbic acid.", J. CELL. BIOCHEM., vol. 66, 1997, pages 394 - 403, XP002993352
CHEN G. ET AL.: "Redifferentation of dediferentiated bovine chondrocytes when cultured in vitro on a PLGA-collagen hybrid mesh.", FEBS LETT., vol. 542, 2003, pages 95 - 99, XP004422730
PEI M. ET AL.: "Growth factors for sequential cellular de-and re-differentiation in tissue engineering.", BIOCHEM.BIOPHYS. RES.COMMUN., vol. 294, 2002, pages 149 - 154, XP002993353
SHUM L. ET AL.: "BMP4 promotes chondrocyte proliferation and hypertrophy in the endochondral cranial base.", INT.J.DEV.BIOL., vol. 47, 2003, pages 423 - 431, XP002993354
See also references of EP 1792977A4
Attorney, Agent or Firm:
Suzuye, Takehiko c/o SUZUYE & SUZUYE (1-12-9 Toranomo, Minato-ku Tokyo, JP)
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