Title:
DIVISION/DETACHMENT MEMBER, CULTURE VESSEL, AND DEVICE
Document Type and Number:
WIPO Patent Application WO/2016/157326
Kind Code:
A1
Abstract:
Provided is a cell colony division/detachment member which makes it possible to detach uniform cell colonies. A division/detachment member is constructed, which is equipped with: a division section 7 which can divide a cell colony 2 into pieces each having a proper size on a culture surface 1 on which the cell colony 2 is cultured, prior to the detachment of the cell colony 2 from the culture surface 1; an enzyme introduction section 6 through which a detaching enzyme 4 capable of detaching cells is introduced into division gaps formed after the division by the division section 7; and a detachment section 8 which can detach the divided cell colonies completely. A cell culture device is so configured that a cell colony can be divided/detached using the member. Therefore, the cell culture device enables the culturing of cells with high efficiency and with less damage to the cells.
Inventors:
ZHOU GUANGBIN (JP)
SUZUKI DAISUKE (JP)
MATSUMOTO ERINO (JP)
SAITO HIKARU (JP)
SUZUKI DAISUKE (JP)
MATSUMOTO ERINO (JP)
SAITO HIKARU (JP)
Application Number:
PCT/JP2015/059731
Publication Date:
October 06, 2016
Filing Date:
March 27, 2015
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12M3/00; C12M3/08; C12N5/071
Foreign References:
| CN102120983A | 2011-07-13 | |||
| JP2008508957A | 2008-03-27 | |||
| JP2531969Y2 | 1997-04-09 | |||
| JP2001275659A | 2001-10-09 | |||
| JP2004089095A | 2004-03-25 | |||
| JP2008054690A | 2008-03-13 |
Other References:
MAGDALINE COSTA, CURRENT PROTOCOLS IN STEM CELL BIOLOGY, 2008
LISA M HOFFMAN, NATURE BIOTECHNOLOGY, vol. 23, no. 6, 2005, pages 699 - 708
LISA M HOFFMAN, NATURE BIOTECHNOLOGY, vol. 23, no. 6, 2005, pages 699 - 708
Attorney, Agent or Firm:
POLAIRE I. P. C. (JP)
Polaire Intellectual Property Corporation (JP)
Polaire Intellectual Property Corporation (JP)
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