Title:
MESENCHYMAL STEM CELL AND EXOSOME THEREOF OBTAINED BY TREATMENT OF AT LEAST TWO CYTOKINES OF IL4, IL21, AND IL27, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/041162
Kind Code:
A1
Abstract:
Disclosed are a mesenchymal stem cell and an exosome thereof obtained by treatment of at least two cytokines of IL4, IL21, and IL27, and use thereof, which relate to the technical field of biomedicine. The mesenchymal stem cell and the exosome thereof are obtained by pretreating a mesenchymal stem cell with a cytokine composition. The way to obtain the mesenchymal stem cell by pretreatment is as follows: carrying out staged treatment and stimulation on a mesenchymal stem cell by using a complete culture medium containing a cytokine composition to obtain the energized mesenchymal stem cell. The way to obtain the exosome by pretreatment is as follows: carrying out staged treatment and stimulation on a mesenchymal stem cell by using a complete culture medium containing a cytokine composition, and then replacing the complete culture medium with a basic culture medium for starvation treatment.
Inventors:
LIAO YAN (CN)
FU ZEQIN (CN)
LI DUANDUAN (CN)
YANG YULIN (CN)
HUANG YINFU (CN)
CAI CHEGUO (CN)
HU JUANYUAN (CN)
FU ZEQIN (CN)
LI DUANDUAN (CN)
YANG YULIN (CN)
HUANG YINFU (CN)
CAI CHEGUO (CN)
HU JUANYUAN (CN)
Application Number:
PCT/CN2023/102557
Publication Date:
February 29, 2024
Filing Date:
June 27, 2023
Export Citation:
Assignee:
SHENZHEN BEIKE BIOTECHNOLOLGY CO LTD (CN)
International Classes:
C12N5/0775; A61K35/28; A61P29/00; A61P37/02
Domestic Patent References:
| WO2014093948A1 | 2014-06-19 |
Foreign References:
| CN103566357A | 2014-02-12 | |||
| CN105326864A | 2016-02-17 | |||
| US20210052641A1 | 2021-02-25 | |||
| CN115161276A | 2022-10-11 |
Other References:
YANG CHUN-JUAN, YANG JING-HAN, ZHANG LI-LI, ZHANG JIAO-JIAO, WANG JING-HUA, SUN JIA-MEI, XU DONG-HUA: "Immunomodulatory effect and application of mesenchymal stem cells in systemic lupus erythematosus", CHINESE JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY., vol. 15, no. 6, 1 December 2021 (2021-12-01), pages 684 - 689, XP093144373, DOI: 10.3969/j.issn.1673-8705.2021.06.014
WANG JIE, YU LIMEI: "Immunomodulatory characteristics of mesenchymal stem cells mediated by inflammatory factors", CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH JUNE, vol. 24, no. 13, 1 January 2020 (2020-01-01), pages 2108 - 2113, XP093144375, ISSN: 2095-4344, DOI: 10.3969/j.issn.2095-4344.2054
LI AIFEN, GUO FENGBIAO, PAN QUANREN, CHEN SHUXIAN, CHEN JIAXUAN, LIU HUA-FENG, PAN QINGJUN: "Mesenchymal Stem Cell Therapy: Hope for Patients With Systemic Lupus Erythematosus", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 12, 1 September 2021 (2021-09-01), Lausanne, CH , pages 728190, XP093144376, ISSN: 1664-3224, DOI: 10.3389/fimmu.2021.728190
LI JICUI, LUO MANYU, LI BING, LOU YAN, ZHU YUEXIN, BAI XUE, SUN BAICHAO, LU XUEHONG, LUO PING: "Immunomodulatory Activity of Mesenchymal Stem Cells in Lupus Nephritis: Advances and Applications", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 13, 1 March 2022 (2022-03-01), Lausanne, CH , pages 843192, XP093144377, ISSN: 1664-3224, DOI: 10.3389/fimmu.2022.843192
WANG JIE, YU LIMEI: "Immunomodulatory characteristics of mesenchymal stem cells mediated by inflammatory factors", CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH JUNE, vol. 24, no. 13, 1 January 2020 (2020-01-01), pages 2108 - 2113, XP093144375, ISSN: 2095-4344, DOI: 10.3969/j.issn.2095-4344.2054
LI AIFEN, GUO FENGBIAO, PAN QUANREN, CHEN SHUXIAN, CHEN JIAXUAN, LIU HUA-FENG, PAN QINGJUN: "Mesenchymal Stem Cell Therapy: Hope for Patients With Systemic Lupus Erythematosus", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 12, 1 September 2021 (2021-09-01), Lausanne, CH , pages 728190, XP093144376, ISSN: 1664-3224, DOI: 10.3389/fimmu.2021.728190
LI JICUI, LUO MANYU, LI BING, LOU YAN, ZHU YUEXIN, BAI XUE, SUN BAICHAO, LU XUEHONG, LUO PING: "Immunomodulatory Activity of Mesenchymal Stem Cells in Lupus Nephritis: Advances and Applications", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 13, 1 March 2022 (2022-03-01), Lausanne, CH , pages 843192, XP093144377, ISSN: 1664-3224, DOI: 10.3389/fimmu.2022.843192
Attorney, Agent or Firm:
SHENZHEN KOZE PATENT AGENCY OFFICE (CN)
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