METABOLICALLY ENHANCED CYANOBACTERIAL CELL FOR THE PRODUCTION OF ETHANOL

WIPO Patent Application WO/2014/198964

A3

A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The alcohol dehydrogenase enzyme has (i) a Michaelis constant Km for NADPH which is lower than the Michaelis constant Km for NADH of the alcohol dehydrogenase enzyme, and (ii) a Michaelis constant Km for acetaldehyde which is lower than 0.08 ⋅10-3 M. Alternatively, the alcohol dehydrogenase enzyme has a Michaelis constant Km for acetaldehyde which is higher than 0.65 ⋅10-3 M but lower than 10 ⋅10-3 M in combination with a Michaelis constant Km for ethanol which is higher than 20 ⋅10-3 M. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

JP7001053	Glycation reaction solution, saccharifying enzyme composition, sugar production method and ethanol production method
WO/2013/050583	HIGH EFFICIENCY BIOFUEL PRODUCTION USING EXTREMELY THERMOPHILIC BACTERIA
WO/2012/141547	ENHANCED-ACTIVITY MODIFIED BETA-GLUCOSIDASE AND A MANUFACTURING METHOD FOR BIOETHANOL USING SAME

DUEHRING ULF (DE)
ENKE HEIKE (DE)
ZIEGLER KARL (DE)
SCHWECKE TORSTEN

PCT/EP2014/062594

February 05, 2015

June 16, 2014

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ALGENOL BIOFUELS INC (US)
DUEHRING ULF (DE)

C12P7/06

WO2007084477A1	2007-07-26
WO2009098089A2	2009-08-13
WO2014100799A2	2014-06-26

GAO ET AL: "Photosynthetic production of enthanol from carbon dioxide in genetically engineered cyanobacteria", 26 September 2012 (2012-09-26), XP002731716, Retrieved from the Internet [retrieved on 20141027]
DATABASE EMBL [online] EBI; 1 January 2006 (2006-01-01), "Arthrospira maxima Fosmid Library", XP002731717, Database accession no. cz682401

EPPING HERMANN FISCHER PATENTANWALTSGESELLSCHAFT MBH (München, DE)

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