Title:
METHOD FOR ACQUIRING ACTIVE INGREDIENT OF ANTI-HEMATOLOGIC TUMOR DRUG AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/102859
Kind Code:
A1
Abstract:
Disclosed is a method for acquiring an active ingredient of a drug capable of treating/preventing hematologic tumors. The method comprises performing three-stage separation on extracellular vesicles, which are secreted by NK-92 cells and NK-92MI cells, to obtain two groups of extracellular vesicles with three sizes: a large size, a medium size and a small size; and then, activity screening is performed on a large sample consisting of miRNA sequences of NK-92 cells and NK-92MI cells and miRNA sequences of the two groups of extracellular vesicles to obtain active miRNA having a strong ability to kill hematologic tumor cells. The active miRNA participates in the regulation of gene expression, regulates the early development of immune cells, affects the development and differentiation of immune cells, and has an effect of treating/preventing hematologic tumors. Moreover, the transfection efficiency of the active miRNA in the liposome is high, and an anti-tumor effect obtained after transfection is obvious.
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Inventors:
SUI MEIHUA (CN)
CHEN YU (CN)
LU HUAN (CN)
LI CHEN (CN)
WU HAO (CN)
CHEN YU (CN)
LU HUAN (CN)
LI CHEN (CN)
WU HAO (CN)
Application Number:
PCT/CN2021/136908
Publication Date:
June 15, 2023
Filing Date:
December 09, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C12N15/113; A61K31/711; A61P7/00; A61P35/00
Domestic Patent References:
| WO2017173367A2 | 2017-10-05 |
Foreign References:
| US20160015748A1 | 2016-01-21 | |||
| US20150057173A1 | 2015-02-26 | |||
| US20200131575A1 | 2020-04-30 | |||
| US20120015351A1 | 2012-01-19 | |||
| CN106191251A | 2016-12-07 | |||
| CN112980947A | 2021-06-18 |
Attorney, Agent or Firm:
HANGZHOU HUACHEN JOINT INTELLECTUAL PROPERTY AGENCY (SPECIAL GENERAL PARTNERSHIP) (CN)
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