Title:
METHOD FOR ALLERGEN DETECTION BY IMMUNOCHROMATOGRAPHIC ASSAY
Document Type and Number:
WIPO Patent Application WO/2017/126525
Kind Code:
A1
Abstract:
Provided is a method for allergen detection by immunochromatographic assay that is specific to allergen detection in a liquid sample such as a swab solution or rinse water and enables the quick and accurate detection of allergens without involving extraction or heat treatment. Also provided is an allergen detection kit that can be used in said method. In the method for allergen detection, immunochromatographic assay is performed on a liquid sample in the presence of sodium dodecyl sulfate (SDS). The immunochromatographic assay is a means of qualitatively or quantitatively detecting allergens in a liquid sample, wherein a colloidal gold–labeled antibody obtained by binding colloidal gold to a monoclonal antibody that can recognize both modified and unmodified allergens serves as a mobile phase, a monoclonal antibody that can recognize both modified and unmodified allergens and that recognizes a different epitope than the aforementioned colloidal gold–labeled antibody serves as a stationary phase, and a complex of a specific allergen and the colloidal gold–labeled antibody migrates and binds specifically to the monoclonal antibody of the stationary phase.
Inventors:
KATO SHIGEKI (JP)
KATO AYAKO (JP)
KATO AYAKO (JP)
Application Number:
PCT/JP2017/001473
Publication Date:
July 27, 2017
Filing Date:
January 18, 2017
Export Citation:
Assignee:
PRIMA MEAT PACKERS LTD (JP)
International Classes:
G01N33/543; G01N33/50
Foreign References:
| JP2007278773A | 2007-10-25 | |||
| JP5735411B2 | 2015-06-17 | |||
| JP2007108169A | 2007-04-26 | |||
| JP2010127922A | 2010-06-10 |
Other References:
"FASTKIT SLIM EGG", INSTRUCTION MANUAL, August 2009 (2009-08-01), XP055400419, Retrieved from the Internet [retrieved on 20170222]
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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