Title:
METHOD FOR AMPLIFYING CELL USING AMINO ACID PREPARATION
Document Type and Number:
WIPO Patent Application WO/2014/188994
Kind Code:
A1
Abstract:
Provided is a method for producing a functional cell from an undifferentiated organ cell with high efficiency and at low cost.
A method for amplifying an undifferentiated liver cell, which comprises culturing the undifferentiated liver cell in the presence of a branched amino acid at a concentration of 1 mM or more. Also provided are: a method for inducing the differentiation into a liver cell; a method for preparing a liver cell; a method for selecting a cell that can be differentiated into a liver cell; a culture medium; a promoter for the proliferation of an undifferentiated liver cell; and an inducer of the differentiation into a liver cell.
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Inventors:
TANIGUCHI HIDEKI (JP)
TAKEBE TAKANORI (JP)
UENO YASUHARU (JP)
KOIKE HIROYUKI (JP)
TAKEBE TAKANORI (JP)
UENO YASUHARU (JP)
KOIKE HIROYUKI (JP)
Application Number:
PCT/JP2014/063181
Publication Date:
November 27, 2014
Filing Date:
May 19, 2014
Export Citation:
Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY (JP)
International Classes:
C12N5/071; C12N1/00
Domestic Patent References:
| WO2013018851A1 | 2013-02-07 | |||
| WO2012111790A1 | 2012-08-23 | |||
| WO2005047454A1 | 2005-05-26 |
Foreign References:
| JP3696297B2 | 2005-09-14 |
Other References:
NANHO HO ET AL., SAIBO BAIYO NARUHODO Q&A IGAI TO SHIRANAI KISO CHISHIKI + TOSSA NI YAKUDATSU TECHNIQUE, 10 February 2005 (2005-02-10), pages 82 - 83
GIULIO MARCHESINI ET AL.: "Branched-Chain Amino Acid Supplementation in Patients with Liver Diseases", J. NUTRITION, vol. 135, no. 6, 2005, pages 1596S - 1601S
TOMOAKI TOMIYA ET AL.: "Branched-chain amino acids, hepatocyte growth factor and protein production in the liver", HEPATOLOGY RESEARCH, vol. 30, 2004, pages 14 - 18
TOMOAKI TOMIYA ET AL.: "Leucine no HGF Sansei Sokushin Sayo to sono Rinshoteki Igi", JAPANESE JOURNAL OF NUTRITIONAL ASSESSMENT, vol. 20, no. 5, 2003, pages 495 - 498
CHIORI IJICHI ET AL.: "Branched-chain amino acids promote albumin synthesis in rat primary hepatocytes through the mTOR signal transduction system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 303, no. 1, 2003, pages 59 - 64, XP008162560, DOI: doi:10.1016/S0006-291X(03)00295-X
GIULIO MARCHESINI ET AL.: "Branched-Chain Amino Acid Supplementation in Patients with Liver Diseases", J. NUTRITION, vol. 135, no. 6, 2005, pages 1596S - 1601S
TOMOAKI TOMIYA ET AL.: "Branched-chain amino acids, hepatocyte growth factor and protein production in the liver", HEPATOLOGY RESEARCH, vol. 30, 2004, pages 14 - 18
TOMOAKI TOMIYA ET AL.: "Leucine no HGF Sansei Sokushin Sayo to sono Rinshoteki Igi", JAPANESE JOURNAL OF NUTRITIONAL ASSESSMENT, vol. 20, no. 5, 2003, pages 495 - 498
CHIORI IJICHI ET AL.: "Branched-chain amino acids promote albumin synthesis in rat primary hepatocytes through the mTOR signal transduction system", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 303, no. 1, 2003, pages 59 - 64, XP008162560, DOI: doi:10.1016/S0006-291X(03)00295-X
Attorney, Agent or Firm:
MAYAMA, Setsuko et al. (JP)
Setsuko Mayama (JP)
Setsuko Mayama (JP)
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