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Patent Searching and Data


Title:
METHOD FOR AMPLIFYING TARGET NUCLEIC ACID AND COMPOSITION FOR AMPLIFYING TARGET NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2019/107893
Kind Code:
A2
Abstract:
The present invention relates to a method for detecting a target nucleic acid, which induces an arbitrary surrogate target to be amplified together in the presence of the target nucleic acid. More specifically, the present invention relates to a method for inducing production of a fusion amplicon of a target nucleic acid and an arbitrary surrogate target when the target nucleic acid is present, and amplifying the fusion amplicon so as to implement high efficiency and high sensitivity of a target nucleic acid detection, and to a composition for a polymerase chain reaction (PCR) for implementing the method. The method for detecting a target nucleic acid according to the present invention can arbitrarily control a part of a base sequence of a polymerase chain reaction product, and consequently, dependence on a base sequence of the target nucleic acid is minimized so that the detection of a target nucleic acid can be effectively performed even if detecting the target nucleic acid is difficult because a portion thereof has an excessively large proportion of guanine/cytosine. Also, since it is possible to insert an arbitrary base sequence between a base sequence region complementary to the target nucleic acid in the surrogate target and a base sequence region complementary to an amplification primer, a universal probe hybridized with such arbitrary base sequence regions can be produced, and thus there is an advantage in that the universal probe can be continuously used even if the base sequence of the target nucleic acid is changed. In addition, the method for detecting a target nucleic acid according to the present invention can easily independently control only the sensitivity of the target nucleic acid detection by adjusting an injection amount of the surrogate target, and can minimize fluctuation of detection efficiency during an optimization process of sensitivity in the development of a diagnostic product, and therefore the method is useful for molecular diagnosis, prenatal diagnosis, early diagnosis, cancer diagnosis, genetic diagnosis, diagnosis of genetic traits, diagnosis of infectious bacteria, identification of drug resistant bacteria, forensic medicine, and identification of species of organisms.

Inventors:
PARK, Jaejin (117-201, 21 Wolgye-ro 45-gil,Nowon-gu, Seoul, 01883, KR)
KIM, Minseo (213-106, 122 Hambak-ro,Gimhae-si, Gyeongsangnam-do, 50958, KR)
Application Number:
KR2018/014776
Publication Date:
June 06, 2019
Filing Date:
November 28, 2018
Export Citation:
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Assignee:
PANAGENE INC. (54 Techno 10-ro, Yuseong-gu, Daejeon, 34027, KR)
International Classes:
C12Q1/6813; C12Q1/686; C12Q1/6876
Attorney, Agent or Firm:
LEE, Cheo Young et al. (11F, 123 Teheran-ro,Gangnam-gu, Seoul, 06133, KR)
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