Title:
METHOD FOR ANALYZING ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2022/270126
Kind Code:
A1
Abstract:
The present invention provides a method that enables quantification of an antibody by mass spectrometry without constructing a calibration curve using the target antibody. Provided is a method for analyzing an antibody, said method comprising digesting a biological protein and the target antibody with a protease, mass spectrometrically analyzing peptide fragments of the thus digested biological protein and target antibody, and quantifying the target antibody on the basis of the ratio of the detection intensity of the biological protein and the detection intensity of the target antibody obtained by the mass spectrometry, wherein: the digestion with the protease comprises immobilizing the biological protein and the target antibody in pores of a carrier and placing the carrier closer to fine particles which have a diameter greater than the diameter of the pores and on the surface of which the protease is immobilized; and the biological protein has a domain which can be immobilized in the pores.
Inventors:
SHIMADA TAKASHI (US)
IWAMOTO NORIKO (US)
IWAMOTO NORIKO (US)
Application Number:
PCT/JP2022/017389
Publication Date:
December 29, 2022
Filing Date:
April 08, 2022
Export Citation:
Assignee:
SHIMADZU CORP (JP)
International Classes:
G01N27/62
Domestic Patent References:
| WO2018167847A1 | 2018-09-20 | |||
| WO2019155576A1 | 2019-08-15 | |||
| WO2019130549A1 | 2019-07-04 | |||
| WO2016194114A1 | 2016-12-08 | |||
| WO2015033479A1 | 2015-03-12 | |||
| WO2015033479A1 | 2015-03-12 | |||
| WO2016194114A1 | 2016-12-08 | |||
| WO2016143223A1 | 2016-09-15 | |||
| WO2016143224A1 | 2016-09-15 | |||
| WO2016143226A1 | 2016-09-15 | |||
| WO2016143227A1 | 2016-09-15 | |||
| WO2019130549A1 | 2019-07-04 | |||
| WO2019155576A1 | 2019-08-15 |
Foreign References:
| US20190085056A1 | 2019-03-21 |
Other References:
IWAMOTO N: "Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano- surface and molecular-orientation limited proteolysis", ANALYST, vol. 139, no. 3, 7 February 2014 (2014-02-07), pages 576 - 80
IWAMOTO N, BIOANALYSIS
IWAMOTO N, BIOLOGICAL&PHARMACEUTICAL BULLETIN, 2016
IWAMOTO N, BIOANALYSIS
IWAMOTO N, BIOLOGICAL&PHARMACEUTICAL BULLETIN, 2016
Attorney, Agent or Firm:
FUKAMI PATENT OFFICE, P.C. (JP)
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