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Patent Searching and Data


Title:
METHOD FOR ANALYZING COMPONENTS
Document Type and Number:
WIPO Patent Application WO/1990/000202
Kind Code:
A1
Abstract:
This invention relates to a method for quantitatively determining components, which comprises converting NAD(P)H existing in a sample or being produced from components other than the intended component by a reaction to NA(D)P with oxidized type glutathione and glutathione reductase, decomposing remaining oxidized type glutathione by the action of gamma-glutamyltranspeptidase in the presence or absence of a mercapto compound, and determining the intended component by utilizing the reaction of producing NAD(P)H. This method enables the amylase activity or the like of a component of a living body containing maltose or glucose to be determined accurately.

Inventors:
MIIKE AKIRA (JP)
TATANO TOSHIO (JP)
Application Number:
PCT/JP1989/000652
Publication Date:
January 11, 1990
Filing Date:
June 29, 1989
Export Citation:
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Assignee:
KYOWA MEDEX CO LTD (JP)
International Classes:
C12Q1/00; C12Q1/40; C12Q1/48; (IPC1-7): C12Q1/26; C12Q1/34; C12Q1/40; C12Q1/46; C12Q1/48
Foreign References:
JPH06214797A1994-08-05
Other References:
Can. J. Biochem and Physiology, Vol. 40, page 1129, (1962), HAGEN, J.H. and HAGEN, P.B.
Maruo Fumiharu, Tamiya Nobuo, Asakura Kunizo Kanshu (Koso Handbook) 1 December 1982 (01. 12. 82) ASAKURA SHOTEN p. 134-135, 259
See also references of EP 0392021A4
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