Title:
METHOD FOR ANALYZING G-PROTEIN CONJUGATED RECEPTOR
Document Type and Number:
WIPO Patent Application WO/2023/243495
Kind Code:
A1
Abstract:
Provided is a method capable of expressing and activating the function of a G-protein conjugated receptor (GPCR). A method for expressing a GPCR polypeptide comprises expressing, in a cell, a GPCR polypeptide comprising an amino acid sequence obtained by modifying at least one of amino acid residues different from a consensus amino acid sequence in the amino acid sequence of a target GPCR (excluding an olfactory receptor) with an amino acid residue in the consensus amino acid sequence at a position corresponding to that described above, wherein said consensus amino acid sequence is an amino acid sequence derived from the alignment of the amino acid sequence of the target GPCR and the amino acid sequence of the GPCR encoded by the ortholog of the target GPCR in a vertebrate.
Inventors:
YOSHIKAWA KEIICHI (JP)
Application Number:
PCT/JP2023/021052
Publication Date:
December 21, 2023
Filing Date:
June 06, 2023
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/12; C07K14/47; C07K14/705; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/63; C12N15/85; C12P21/02; C12Q1/02; C12Q1/06; C12Q1/6897; C40B40/08; G01N33/53; G01N33/566
Domestic Patent References:
| WO2020006108A2 | 2020-01-02 |
Foreign References:
| JP2006051020A | 2006-02-23 | |||
| JP2005500817A | 2005-01-13 | |||
| JP2003530098A | 2003-10-14 | |||
| JP2002511871A | 2002-04-16 | |||
| JP2023002484A | 2023-01-10 |
Other References:
IKEGAMI KENTARO, DE MARCH CLAIRE A., NAGAI MAIRA H., GHOSH SOUMADWIP, DO MATTHEW, SHARMA RUCHIRA, BRUGUERA ELISE S., LU YUEYANG ER: "Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 117, no. 6, 11 February 2020 (2020-02-11), pages 2957 - 2967, XP055894313, ISSN: 0027-8424, DOI: 10.1073/pnas.1915520117
Attorney, Agent or Firm:
ARUGA PATENT OFFICE (JP)
Download PDF: