Title:
METHOD FOR ANALYZING HIGHER-ORDER STRUCTURE OF RNA
Document Type and Number:
WIPO Patent Application WO/2022/209428
Kind Code:
A1
Abstract:
The present disclosure provides a feature of efficiently detecting a wider variety of RNA higher-order structures including non-Watson-Crick base pair-type higher order structures. The method for analyzing the RNA higher-order structure according to the present disclosure comprises: a step for readying a compound in which a target-binding moiety Sm and an RNA-modifying moiety Y are linked by a linker L, and bringing the compound and one or a plurality of RNAs into contact; a step for determining the base sequence of the RNA that had come into contact with the compound; and a step for determining, on the basis of the determined base sequence, the position and/or region on the RNA that interacts with the target-binding moiety.
Inventors:
KOMATSU RICHARD KAORU (JP)
MIYASHITA EMI (JP)
ONIZUKA KAZUMITSU (JP)
NAGATSUGI FUMI (JP)
MIYASHITA EMI (JP)
ONIZUKA KAZUMITSU (JP)
NAGATSUGI FUMI (JP)
Application Number:
PCT/JP2022/007117
Publication Date:
October 06, 2022
Filing Date:
February 22, 2022
Export Citation:
Assignee:
XFOREST THERAPEUTICS CO LTD (JP)
International Classes:
C07D239/90; C12Q1/68; C12N15/09; C12Q1/6806; C12Q1/6869; G16B15/10
Other References:
ONIZUKA K., ET AL.: "Reactive OFF-ON type alkylating agents for higher-ordered structures of nucleic acids", NUCLEIC ACIDS RESEARCH, vol. 47, no. 13, 12 June 2019 (2019-06-12), pages 6578 - 6589, XP055972125, DOI: 10.1093/nar/gkz512
SATO, NORIHIRO; SASAKI, NORIHIRO, TSUJI, YOSHIHIRO: "2 PB-019 Investigation of the selective chemical modification within a hydrophobic pocket in higher order structures of nucleic acid", LECTURE PREPRINTS OF THE CHEMICAL SOCIETY OF JAPAN (CSJ); TOKYO, JAPAN; MARCH 26-29, 2015, vol. 95, 11 March 2015 (2015-03-11) - 29 March 2015 (2015-03-29), pages 939, XP009540090
HAZEMI MADOKA E.; ONIZUKA KAZUMITSU; KOBAYASHI TOMOHITO; USAMI AKIRA; SATO NORIHIRO; NAGATSUGI FUMI: "Vinyldiaminotriazine-acridine conjugate as G-quadruplex alkylating agent", BIOORGANIC & MEDICINAL CHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 26, no. 12, 19 May 2018 (2018-05-19), AMSTERDAM, NL, pages 3551 - 3558, XP085408706, ISSN: 0968-0896, DOI: 10.1016/j.bmc.2018.05.030
SATO, NORIHIRO; SASAKI, NORIHIRO, TSUJI, YOSHIHIRO: "2 PB-019 Investigation of the selective chemical modification within a hydrophobic pocket in higher order structures of nucleic acid", LECTURE PREPRINTS OF THE CHEMICAL SOCIETY OF JAPAN (CSJ); TOKYO, JAPAN; MARCH 26-29, 2015, vol. 95, 11 March 2015 (2015-03-11) - 29 March 2015 (2015-03-29), pages 939, XP009540090
HAZEMI MADOKA E.; ONIZUKA KAZUMITSU; KOBAYASHI TOMOHITO; USAMI AKIRA; SATO NORIHIRO; NAGATSUGI FUMI: "Vinyldiaminotriazine-acridine conjugate as G-quadruplex alkylating agent", BIOORGANIC & MEDICINAL CHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 26, no. 12, 19 May 2018 (2018-05-19), AMSTERDAM, NL, pages 3551 - 3558, XP085408706, ISSN: 0968-0896, DOI: 10.1016/j.bmc.2018.05.030
Attorney, Agent or Firm:
MEBUKI IP LAW FIRM et al. (JP)
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