Title:
METHOD FOR ANALYZING INTER-DNA INTERACTION
Document Type and Number:
WIPO Patent Application WO/2017/090543
Kind Code:
A1
Abstract:
Provided is a method for analyzing inter-DNA interaction comprising: (1) a step for preparing a fragmented DNA by ultrasonication or by a partial decomposition process using an endonuclease without DNA sequence specificity, the DNA to be fragmented being in a condition in which the DNA to be analyzed and another DNA are interacting with each other; (2) a step for ligating the fragmented DNA; (3) a step for, from ligated DNA, amplifying and/or purifying a composite containing the DNA to be analyzed and the DNA interacting with said DNA; and (4) a step for analyzing interacting DNA in the amplified and/or purified composite. The method does not require costly equipment or special skills and can be easily implemented by any researcher. Furthermore, the method for analyzing inter DNA-interaction obtains highly reliable results and can be implemented without the use of a restriction enzyme.
Inventors:
FUJII HODAKA (JP)
FUJITA TOSHITSUGU (JP)
FUJITA TOSHITSUGU (JP)
Application Number:
PCT/JP2016/084358
Publication Date:
June 01, 2017
Filing Date:
November 18, 2016
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
| WO2011021684A1 | 2011-02-24 | |||
| WO2014125668A1 | 2014-08-21 |
Foreign References:
| JP2009500033A | 2009-01-08 |
Other References:
CAI M. ET AL.: "Analysis of a transgenic Oct4 enhancer reveals high fidelity long-range chromosomal interactions", SCIENTIFIC REPORTS, vol. 5, no. 14558, 5 October 2015 (2015-10-05), pages 2 - 10
GAO F. ET AL.: "Comparative analysis of 4C-Seq data generated from enzyme-based and sonication-based methods", BMC GENOMICS, vol. 14, no. 345, 2013, XP021151972
GAO F. ET AL.: "Comparative analysis of 4C-Seq data generated from enzyme-based and sonication-based methods", BMC GENOMICS, vol. 14, no. 345, 2013, XP021151972
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
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