Title:
METHOD FOR ANALYZING ISOASPARTIC ACID AND MASS SPECTROMETER
Document Type and Number:
WIPO Patent Application WO/2021/010401
Kind Code:
A1
Abstract:
A method of analyzing isoaspartic acid by dissociating precursor ions derived from sample components and analyzing a generated product ions, the method comprising a step of generating the product ions by irradiating the precursor ions with hydrogen radicals or radicals having oxidizing ability to dissociate the precursor ions, separating and detecting the product ions according to a mass charge ratio, and specifying a product ion derived from isoaspartic acid on the basis of the mass charge ratio of the product ion.
Inventors:
TAKAHASHI HIDENORI (JP)
ASAKAWA DAIKI (JP)
ASAKAWA DAIKI (JP)
Application Number:
PCT/JP2020/027388
Publication Date:
January 21, 2021
Filing Date:
July 14, 2020
Export Citation:
Assignee:
SHIMADZU CORP (JP)
AIST (JP)
AIST (JP)
International Classes:
G01N27/62; H01J49/26
Domestic Patent References:
| WO2018186286A1 | 2018-10-11 | |||
| WO2015133259A1 | 2015-09-11 |
Foreign References:
| JP2018044826A | 2018-03-22 | |||
| JP2017191737A | 2017-10-19 |
Other References:
NOUE KOICHI, HOSAKA DAIJU, MOCHIZUKI NANA, AKATSU HIROYASU, TSUTSUMIUCHI KANAME, HASHIZUME YOSHIO, MATSUKAWA NORIYUKI, YAMAMOTO TA: "Simultaneous Determination of Post-Translational Racemization and Isomerization of N-Terminal Amyloid-beta in Alzheimer' s Brain Tissues by Covalent Chiral Derivatized Ultraperformance Liquid Chromatography Tandem Mass Spectrometry", ANALYTICAL CHEMISTRY, vol. 86, 27 November 2013 (2013-11-27), pages 797 - 804, XP055788930, DOI: org/10.1021/ac403315h
WANG SHUNHAI, KALTASHOV IGOR A.: "An 18 O-Labeling Assisted LC/MS Method for Assignment of Aspartyl/Isoaspartyl Products from Asn Deamidation and Asp Isomerization in Proteins", ANALYTICAL CHEMISTRY, vol. 85, no. 13, 28 May 2013 (2013-05-28), pages 6446 - 6452, XP055788935, DOI: 10.1021/ac400984r
DEGRAAN-WEBER ET AL.: "Distinguishing aspartic and isoaspartic acids in peptides by several mass spectrometric fragmentation methods", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27, no. 12, 9 September 2016 (2016-09-09), pages 2041 - 2053, XP036315862, DOI: 10.1007/s13361-016-1487-9
KEMPKES, L. J.M ET AL.: "Deamidation Reactions of Asparagine- and Glutamine-Containing Dipeptides Investigated by Ion Spectroscopy", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27, 13 September 2016 (2016-09-13), pages 1855 - 1869, XP036073454, DOI: 10.1007/s13361-016-1462-5
TAKADA TAKUMI: "Study on mechanism of cataract development caused by spontaneous chemical modification of amino acids in protein constituting lens", JOURNAL OF JAPAN CATARACT SOCIETY, vol. 30, 2018, pages 7 - 12
KEMPKES, L. J.MARTENS, J.GRZETIC, J.BERDEN, G.OOMENS, J.: "Deamidation Reactions of Asparagine-and Glutamine-Containing Dipeptides Investigated by Ion Spectroscopy", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27, no. 11, 2016, pages 1855 - 1869, XP036073454, DOI: 10.1007/s13361-016-1462-5
SHIMABUKUROKASUYAWADA: "Proc. of the 77th JSAP Academic Lecture", September 2016, JAPAN SOCIETY OF APPLIED PHYSICS, article "Development of a Compact Atom Beam Source by Capacitively Coupled Plasma at 2.45GHz"
YUJI SHIMABUKUROHIDENORI TAKAHASHISHINICHI IWAMOTOKOICHI TANAKAMOTOI WADA: "Tandem Mass Spectrometry of Peptide Ions by Microwave Excited Hydrogen and Water Plasmas", ANAL. CHEM., vol. 90, no. 12, 2018, pages 7239 - 7245, XP055787273, DOI: 10.1021/acs.analchem.8b00344
See also references of EP 4001911A4
WANG SHUNHAI, KALTASHOV IGOR A.: "An 18 O-Labeling Assisted LC/MS Method for Assignment of Aspartyl/Isoaspartyl Products from Asn Deamidation and Asp Isomerization in Proteins", ANALYTICAL CHEMISTRY, vol. 85, no. 13, 28 May 2013 (2013-05-28), pages 6446 - 6452, XP055788935, DOI: 10.1021/ac400984r
DEGRAAN-WEBER ET AL.: "Distinguishing aspartic and isoaspartic acids in peptides by several mass spectrometric fragmentation methods", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27, no. 12, 9 September 2016 (2016-09-09), pages 2041 - 2053, XP036315862, DOI: 10.1007/s13361-016-1487-9
KEMPKES, L. J.M ET AL.: "Deamidation Reactions of Asparagine- and Glutamine-Containing Dipeptides Investigated by Ion Spectroscopy", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27, 13 September 2016 (2016-09-13), pages 1855 - 1869, XP036073454, DOI: 10.1007/s13361-016-1462-5
TAKADA TAKUMI: "Study on mechanism of cataract development caused by spontaneous chemical modification of amino acids in protein constituting lens", JOURNAL OF JAPAN CATARACT SOCIETY, vol. 30, 2018, pages 7 - 12
KEMPKES, L. J.MARTENS, J.GRZETIC, J.BERDEN, G.OOMENS, J.: "Deamidation Reactions of Asparagine-and Glutamine-Containing Dipeptides Investigated by Ion Spectroscopy", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 27, no. 11, 2016, pages 1855 - 1869, XP036073454, DOI: 10.1007/s13361-016-1462-5
SHIMABUKUROKASUYAWADA: "Proc. of the 77th JSAP Academic Lecture", September 2016, JAPAN SOCIETY OF APPLIED PHYSICS, article "Development of a Compact Atom Beam Source by Capacitively Coupled Plasma at 2.45GHz"
YUJI SHIMABUKUROHIDENORI TAKAHASHISHINICHI IWAMOTOKOICHI TANAKAMOTOI WADA: "Tandem Mass Spectrometry of Peptide Ions by Microwave Excited Hydrogen and Water Plasmas", ANAL. CHEM., vol. 90, no. 12, 2018, pages 7239 - 7245, XP055787273, DOI: 10.1021/acs.analchem.8b00344
See also references of EP 4001911A4
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
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