Title:
METHOD FOR ANALYZING MICROBIAL FLORA
Document Type and Number:
WIPO Patent Application WO/2020/262413
Kind Code:
A1
Abstract:
Provided is a method that is for analyzing microbial flora and that comprises (1) a step for dissolving a probe that can non-specifically interact with a plurality of microorganisms in a plurality of solvents having different ion strengths and/or pH values, the probe including (a) a cationic polymer and (b) an environmentally responsive fluorophore; (2) a step for adding a sample to be analyzed containing microbial flora into the plurality of probe solutions prepared in step (1), resulting in non-specific interaction between the probe and the microorganisms in the sample to be analyzed; (3) a step for measuring fluorescence intensities of the plurality of probe solutions into which the sample to be analyzed has been added in step (2); and (4) a step for comparing a fluorescence intensity pattern obtained in step(3) with a fluorescence intensity pattern obtained for a reference sample.
Inventors:
TOMITA SHUNSUKE (JP)
KURITA RYOJI (JP)
TAMAKI HIDEYUKI (JP)
KUSADA HIROYUKI (JP)
KOJIMA NAOSHI (JP)
ISHIHARA SAYAKA (JP)
MIYAZAKI KOYOMI (JP)
YUMOTO ISAO (JP)
KURITA RYOJI (JP)
TAMAKI HIDEYUKI (JP)
KUSADA HIROYUKI (JP)
KOJIMA NAOSHI (JP)
ISHIHARA SAYAKA (JP)
MIYAZAKI KOYOMI (JP)
YUMOTO ISAO (JP)
Application Number:
PCT/JP2020/024687
Publication Date:
December 30, 2020
Filing Date:
June 24, 2020
Export Citation:
Assignee:
AIST (JP)
International Classes:
G01N21/64; C12Q1/04
Domestic Patent References:
| WO2018088510A1 | 2018-05-17 | |||
| WO2017205981A1 | 2017-12-07 |
Foreign References:
| US20110177976A1 | 2011-07-21 | |||
| JP2012132753A | 2012-07-12 | |||
| US20160349245A1 | 2016-12-01 | |||
| JP2007060953A | 2007-03-15 | |||
| JP2016106632A | 2016-06-20 |
Other References:
GATELY, S.: "Human Microbiota and Personalized Cancer Treatmnets: Role of Commensal Microbes in Treatment Outcomes for Cancer Patients", PRECISION MEDICINE IN CANCER THERAPY, vol. 178, 18 June 2019 (2019-06-18), pages 253 - 264
BENJDIA, A. ET AL.: "Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis", NATURE CHEMISTRY, vol. 9, no. 7, 2017, pages 698 - 707, XP055600299, DOI: 10.1038/nchem.2714
LAI, L. A. ET AL.: "Metaproteomics Study of the Gut Microbiome , Functional Proteomics: Methods and Protocols", METHODS IN BIOLOGY, vol. 1871, 1 October 2018 (2018-10-01), pages 123 - 132
BENJDIA, A. ET AL.: "Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis", NATURE CHEMISTRY, vol. 9, no. 7, 2017, pages 698 - 707, XP055600299, DOI: 10.1038/nchem.2714
LAI, L. A. ET AL.: "Metaproteomics Study of the Gut Microbiome , Functional Proteomics: Methods and Protocols", METHODS IN BIOLOGY, vol. 1871, 1 October 2018 (2018-10-01), pages 123 - 132
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
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