METHOD OF ANALYZING NUCLEIC ACID

Title:

METHOD OF ANALYZING NUCLEIC ACID

Document Type and Number:

WIPO Patent Application WO/2007/046158

Kind Code:

A1

Abstract:

By elevating the hybridization efficiency between a primer or a probe and a sample, it becomes possible to stably amplify a nucleic acid in a minor amount and analyze the same at a high sensitivity. Namely, a method of analyzing a nucleic acid characterized by comprising the step of hybridizing at least one first probe having a first sequence complementary to one strand of a double-stranded nucleic acid, a second sequence complementary to the other strand thereof and a third sequence of ligating the first and second sequences with the above-described double-stranded nucleic acid, and the step of hybridizing at least one second probe with the above-described double-stranded nucleic acid.

Inventors:

NAKASHIMA YUKIE (JP)
NAGAI KEIICHI (JP)

Application Number:

PCT/JP2006/304357

Publication Date:

April 26, 2007

Filing Date:

March 07, 2006

Export Citation:

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Assignee:

HITACHI LTD (JP)
NAKASHIMA YUKIE (JP)
NAGAI KEIICHI (JP)

International Classes:

C12N15/09; C12Q1/68

Foreign References:

JP2003500038A	2003-01-07
JP2005502346A	2005-01-27

Other References:

ZHOU G.H. ET AL.: "Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER)", NUCLEIC ACIDS RESEARCH, vol. 29, no. 19, 2001, XP002284369