Title:
METHOD FOR ANALYZING PROTEIN-CONTAINING SAMPLE
Document Type and Number:
WIPO Patent Application WO/2018/088510
Kind Code:
A1
Abstract:
Provided is a method for analyzing a protein-containing sample, the method comprising: (1) a step for dissolving a probe that nonspecifically interacts with a plurality of proteins in a plurality of solvents having different ionic strengths and/or pH's; (2) a step for adding a protein-containing sample to the plurality of probe solutions prepared in step (1) so as to cause the proteins in the sample and the probe to nonspecifically interact with each other; (3) a step for measuring fluorescence strengths of the probe solutions to which the protein-containing sample has been added in step (2); and (4) a step for comparing fluorescent strength patterns obtained in step (3) with a fluorescent strength pattern obtained of a reference sample.
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Inventors:
TOMITA SHUNSUKE (JP)
ISHIHARA SAYAKA (JP)
KURITA RYOJI (JP)
ISHIHARA SAYAKA (JP)
KURITA RYOJI (JP)
Application Number:
PCT/JP2017/040542
Publication Date:
May 17, 2018
Filing Date:
November 10, 2017
Export Citation:
Assignee:
AIST (JP)
International Classes:
G01N21/64; C09K9/02; C09K11/06; G01N33/483; G01N33/68
Foreign References:
| JPH09183853A | 1997-07-15 |
Other References:
TOMITA, S. ET AL.: "The use of an enzyme-based sensor array to fingerprint proteomic signatures of sera from different mammalian species", ANALYTICAL SCIENCES, vol. 32, 10 February 2016 (2016-02-10), pages 237 - 240, XP055501078
TOMITA, S. ET AL.: "Artificial modification of an enzyme for construction of cross-reactive polyion complexes to fingerprint signatures of proteins and mammalian cells", ANALYTICAL CHEMISTRY, vol. 88, no. 18, 22 August 2016 (2016-08-22), pages 9079 - 9086, XP055501080
TOMITA, S. ET AL.: "Environment-sensitive turn-on fluorescent polyamino acid: fingerprinting protein populations with post-translational modifications", ACS APPLIED MATERIALS AND INTERFACES, vol. 9, 19 June 2017 (2017-06-19), pages 22970 - 22976, XP055501084
TOMITA, S. ET AL.: "Artificial modification of an enzyme for construction of cross-reactive polyion complexes to fingerprint signatures of proteins and mammalian cells", ANALYTICAL CHEMISTRY, vol. 88, no. 18, 22 August 2016 (2016-08-22), pages 9079 - 9086, XP055501080
TOMITA, S. ET AL.: "Environment-sensitive turn-on fluorescent polyamino acid: fingerprinting protein populations with post-translational modifications", ACS APPLIED MATERIALS AND INTERFACES, vol. 9, 19 June 2017 (2017-06-19), pages 22970 - 22976, XP055501084
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
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